Dissolution of Biofilm Secreted by Three Different Strains of <i>Pseudomonas aeruginosa</i> with Bromelain, <i>N</i>-Acetylcysteine, and Their Combinations
Bacterial infection of hernia mesh with the formation of biofilms presents a barrier to antibiotic treatment with subsequent surgical intervention and hospitalization. Hence, in the current study, we examined the effect of BromAc, a mucolytic agent, on the dissolution of biofilm formed by three diff...
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MDPI AG
2021-12-01
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| author | Carly J. Carter Krishna Pillai Samina Badar Ahmed H. Mekkawy Javed Akhter Thomas Jefferies Sarah J. Valle David L. Morris |
| author_facet | Carly J. Carter Krishna Pillai Samina Badar Ahmed H. Mekkawy Javed Akhter Thomas Jefferies Sarah J. Valle David L. Morris |
| author_sort | Carly J. Carter |
| collection | DOAJ |
| description | Bacterial infection of hernia mesh with the formation of biofilms presents a barrier to antibiotic treatment with subsequent surgical intervention and hospitalization. Hence, in the current study, we examined the effect of BromAc, a mucolytic agent, on the dissolution of biofilm formed by three different strains of <i>Pseudomonas aeruginosa</i>. <i>Pseudomonas aeruginosa</i> was carefully grown on hernia mesh and treated with various concentrations of bromelain, NAC, and their combinations at 37 °C over 4 h in vitro. Then, the biofilm dissolution activities of the agents were evaluated. Moreover, the combination index (CI) was analyzed to determine the synergy of the bromelain and NAC combination. The results indicated that biofilms were more susceptible to degradation by bromelain, whilst NAC showed growth enhancement in two of the strains. However, in combination (BromAc), the three strains were dramatically affected by the agents, with more than 80% debridement fir a suitable combination of bromelain and NAC that was also strain-specific. Hence, the current study shows that the biofilms formed by these three strains of <i>Pseudomonas aeruginosa</i> were adversely affected by a single treatment of BromAc, with more than 80% debridement, indicating that subsequent treatment may abolish the biofilm completely. |
| first_indexed | 2024-03-10T04:57:06Z |
| format | Article |
| id | doaj.art-4823b9565d5c4c299725577365db9973 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T04:57:06Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-4823b9565d5c4c299725577365db99732023-11-23T02:07:24ZengMDPI AGApplied Sciences2076-34172021-12-0111231138810.3390/app112311388Dissolution of Biofilm Secreted by Three Different Strains of <i>Pseudomonas aeruginosa</i> with Bromelain, <i>N</i>-Acetylcysteine, and Their CombinationsCarly J. Carter0Krishna Pillai1Samina Badar2Ahmed H. Mekkawy3Javed Akhter4Thomas Jefferies5Sarah J. Valle6David L. Morris7Department of Surgery, St George Hospital, Sydney, NSW 2217, AustraliaDepartment of Surgery, St George Hospital, Sydney, NSW 2217, AustraliaDepartment of Surgery, St George Hospital, Sydney, NSW 2217, AustraliaDepartment of Surgery, St George Hospital, Sydney, NSW 2217, AustraliaDepartment of Surgery, St George Hospital, Sydney, NSW 2217, AustraliaSchool of Science, Western Sydney University, Richmond, NSW 2753, AustraliaDepartment of Surgery, St George Hospital, Sydney, NSW 2217, AustraliaDepartment of Surgery, St George Hospital, Sydney, NSW 2217, AustraliaBacterial infection of hernia mesh with the formation of biofilms presents a barrier to antibiotic treatment with subsequent surgical intervention and hospitalization. Hence, in the current study, we examined the effect of BromAc, a mucolytic agent, on the dissolution of biofilm formed by three different strains of <i>Pseudomonas aeruginosa</i>. <i>Pseudomonas aeruginosa</i> was carefully grown on hernia mesh and treated with various concentrations of bromelain, NAC, and their combinations at 37 °C over 4 h in vitro. Then, the biofilm dissolution activities of the agents were evaluated. Moreover, the combination index (CI) was analyzed to determine the synergy of the bromelain and NAC combination. The results indicated that biofilms were more susceptible to degradation by bromelain, whilst NAC showed growth enhancement in two of the strains. However, in combination (BromAc), the three strains were dramatically affected by the agents, with more than 80% debridement fir a suitable combination of bromelain and NAC that was also strain-specific. Hence, the current study shows that the biofilms formed by these three strains of <i>Pseudomonas aeruginosa</i> were adversely affected by a single treatment of BromAc, with more than 80% debridement, indicating that subsequent treatment may abolish the biofilm completely.https://www.mdpi.com/2076-3417/11/23/11388acetylcysteineantibiotic resistancebiofilmbromelain<i>Pseudomonas aeruginosa</i> |
| spellingShingle | Carly J. Carter Krishna Pillai Samina Badar Ahmed H. Mekkawy Javed Akhter Thomas Jefferies Sarah J. Valle David L. Morris Dissolution of Biofilm Secreted by Three Different Strains of <i>Pseudomonas aeruginosa</i> with Bromelain, <i>N</i>-Acetylcysteine, and Their Combinations Applied Sciences acetylcysteine antibiotic resistance biofilm bromelain <i>Pseudomonas aeruginosa</i> |
| title | Dissolution of Biofilm Secreted by Three Different Strains of <i>Pseudomonas aeruginosa</i> with Bromelain, <i>N</i>-Acetylcysteine, and Their Combinations |
| title_full | Dissolution of Biofilm Secreted by Three Different Strains of <i>Pseudomonas aeruginosa</i> with Bromelain, <i>N</i>-Acetylcysteine, and Their Combinations |
| title_fullStr | Dissolution of Biofilm Secreted by Three Different Strains of <i>Pseudomonas aeruginosa</i> with Bromelain, <i>N</i>-Acetylcysteine, and Their Combinations |
| title_full_unstemmed | Dissolution of Biofilm Secreted by Three Different Strains of <i>Pseudomonas aeruginosa</i> with Bromelain, <i>N</i>-Acetylcysteine, and Their Combinations |
| title_short | Dissolution of Biofilm Secreted by Three Different Strains of <i>Pseudomonas aeruginosa</i> with Bromelain, <i>N</i>-Acetylcysteine, and Their Combinations |
| title_sort | dissolution of biofilm secreted by three different strains of i pseudomonas aeruginosa i with bromelain i n i acetylcysteine and their combinations |
| topic | acetylcysteine antibiotic resistance biofilm bromelain <i>Pseudomonas aeruginosa</i> |
| url | https://www.mdpi.com/2076-3417/11/23/11388 |
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