Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas
Synaptotagmin-13 (Syt13) is an atypical member of the vesicle trafficking synaptotagmin protein family. The expression pattern and the biological function of this Ca<sup>2+</sup>-independent protein are not well resolved. Here, we have generated a novel Syt13-Venus fusion (Syt13-VF) fluo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/22/12526 |
| _version_ | 1797509921623769088 |
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| author | Marta Tarquis-Medina Katharina Scheibner Ismael González-García Aimée Bastidas-Ponce Michael Sterr Jessica Jaki Silvia Schirge Cristina García-Cáceres Heiko Lickert Mostafa Bakhti |
| author_facet | Marta Tarquis-Medina Katharina Scheibner Ismael González-García Aimée Bastidas-Ponce Michael Sterr Jessica Jaki Silvia Schirge Cristina García-Cáceres Heiko Lickert Mostafa Bakhti |
| author_sort | Marta Tarquis-Medina |
| collection | DOAJ |
| description | Synaptotagmin-13 (Syt13) is an atypical member of the vesicle trafficking synaptotagmin protein family. The expression pattern and the biological function of this Ca<sup>2+</sup>-independent protein are not well resolved. Here, we have generated a novel Syt13-Venus fusion (Syt13-VF) fluorescence reporter allele to track and isolate tissues and cells expressing Syt13 protein. The reporter allele is regulated by endogenous cis-regulatory elements of <i>Syt13</i> and the fusion protein follows an identical expression pattern of the endogenous Syt13 protein. The homozygous reporter mice are viable and fertile. We identify the expression of the Syt13-VF reporter in different regions of the brain with high expression in tyrosine hydroxylase (TH)-expressing and oxytocin-producing neuroendocrine cells. Moreover, Syt13-VF is highly restricted to all enteroendocrine cells in the adult intestine that can be traced in live imaging. Finally, Syt13-VF protein is expressed in the pancreatic endocrine lineage, allowing their specific isolation by flow sorting. These findings demonstrate high expression levels of Syt13 in the endocrine lineages in three major organs harboring these secretory cells. Collectively, the Syt13-VF reporter mouse line provides a unique and reliable tool to dissect the spatio-temporal expression pattern of Syt13 and enables isolation of Syt13-expressing cells that will aid in deciphering the molecular functions of this protein in the neuroendocrine system. |
| first_indexed | 2024-03-10T05:25:30Z |
| format | Article |
| id | doaj.art-4823f1a594184ccd8b7d615cfc60b619 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T05:25:30Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4823f1a594184ccd8b7d615cfc60b6192023-11-22T23:43:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122221252610.3390/ijms222212526Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and PancreasMarta Tarquis-Medina0Katharina Scheibner1Ismael González-García2Aimée Bastidas-Ponce3Michael Sterr4Jessica Jaki5Silvia Schirge6Cristina García-Cáceres7Heiko Lickert8Mostafa Bakhti9Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, GermanyGerman Center for Diabetes Research (DZD), 85764 Neuherberg, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, GermanyGerman Center for Diabetes Research (DZD), 85764 Neuherberg, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, GermanySynaptotagmin-13 (Syt13) is an atypical member of the vesicle trafficking synaptotagmin protein family. The expression pattern and the biological function of this Ca<sup>2+</sup>-independent protein are not well resolved. Here, we have generated a novel Syt13-Venus fusion (Syt13-VF) fluorescence reporter allele to track and isolate tissues and cells expressing Syt13 protein. The reporter allele is regulated by endogenous cis-regulatory elements of <i>Syt13</i> and the fusion protein follows an identical expression pattern of the endogenous Syt13 protein. The homozygous reporter mice are viable and fertile. We identify the expression of the Syt13-VF reporter in different regions of the brain with high expression in tyrosine hydroxylase (TH)-expressing and oxytocin-producing neuroendocrine cells. Moreover, Syt13-VF is highly restricted to all enteroendocrine cells in the adult intestine that can be traced in live imaging. Finally, Syt13-VF protein is expressed in the pancreatic endocrine lineage, allowing their specific isolation by flow sorting. These findings demonstrate high expression levels of Syt13 in the endocrine lineages in three major organs harboring these secretory cells. Collectively, the Syt13-VF reporter mouse line provides a unique and reliable tool to dissect the spatio-temporal expression pattern of Syt13 and enables isolation of Syt13-expressing cells that will aid in deciphering the molecular functions of this protein in the neuroendocrine system.https://www.mdpi.com/1422-0067/22/22/12526synaptotagmin-13Syt13fluorescent reporterendocrine lineagebrainintestine |
| spellingShingle | Marta Tarquis-Medina Katharina Scheibner Ismael González-García Aimée Bastidas-Ponce Michael Sterr Jessica Jaki Silvia Schirge Cristina García-Cáceres Heiko Lickert Mostafa Bakhti Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas International Journal of Molecular Sciences synaptotagmin-13 Syt13 fluorescent reporter endocrine lineage brain intestine |
| title | Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas |
| title_full | Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas |
| title_fullStr | Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas |
| title_full_unstemmed | Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas |
| title_short | Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas |
| title_sort | synaptotagmin 13 is a neuroendocrine marker in brain intestine and pancreas |
| topic | synaptotagmin-13 Syt13 fluorescent reporter endocrine lineage brain intestine |
| url | https://www.mdpi.com/1422-0067/22/22/12526 |
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