Extensive characterization of the composition and functional activities of five preparations of human platelet lysates for dedicated clinical uses
Human platelet lysates (HPLs), rich in various growth factors and cell growth-promoting molecules, encompass a new range of blood products that are being used for regenerative medicine, cell therapies, and tissue engineering. Well-characterized dedicated preparations, tailor-made to best fit specifi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-02-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2020.1849603 |
| _version_ | 1797684126935941120 |
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| author | Liling Delila Yu-Wen Wu Ouada Nebie Rifa Widyaningrum Ming-Li Chou David Devos Thierry Burnouf |
| author_facet | Liling Delila Yu-Wen Wu Ouada Nebie Rifa Widyaningrum Ming-Li Chou David Devos Thierry Burnouf |
| author_sort | Liling Delila |
| collection | DOAJ |
| description | Human platelet lysates (HPLs), rich in various growth factors and cell growth-promoting molecules, encompass a new range of blood products that are being used for regenerative medicine, cell therapies, and tissue engineering. Well-characterized dedicated preparations, tailor-made to best fit specific therapeutic applications, are needed for optimal clinical efficacy and safety. Here, five types of HPL were prepared from the same platelet concentrates and extensively characterized to determine and compare their proteins, growth factors, cytokines, biochemical profiles, thrombin-generating capacities, thrombin-associated proteolytic activities, phospholipid-associated procoagulant potential, contents of extracellular vesicles expressing phosphatidylserine and tissue factor, and antioxidative properties. Our results revealed that all five HPL preparations contained detectable supraphysiological levels, in the ca. 0.1 ~ 350-ng/ml range, of all growth factors assessed, except insulin-like growth factor-1 detected only in HPL containing plasma. There were significant differences observed among these HPLs in total protein content, fibrinogen, complement components C3 and C4, albumin, and immunoglobulin G, and, most importantly, in their functional coagulant and procoagulant activities and antioxidative capacities. Our data revealed that the biochemical and functional properties of HPL preparations greatly vary depending upon their mode of production, with potential impacts on the safety and efficacy for certain clinical indications. Modes of preparation of HPLs should be carefully designed, and the product properties carefully evaluated based on the intended therapeutic use to ensure optimal clinical outcomes. |
| first_indexed | 2024-03-12T00:25:00Z |
| format | Article |
| id | doaj.art-4825ad2c987f445fae2e366f901ad84f |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:25:00Z |
| publishDate | 2021-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-4825ad2c987f445fae2e366f901ad84f2023-09-15T10:38:09ZengTaylor & Francis GroupPlatelets0953-71041369-16352021-02-0132225927210.1080/09537104.2020.18496031849603Extensive characterization of the composition and functional activities of five preparations of human platelet lysates for dedicated clinical usesLiling Delila0Yu-Wen Wu1Ouada Nebie2Rifa Widyaningrum3Ming-Li Chou4David Devos5Thierry Burnouf6Taipei Medical UniversityTaipei Medical UniversityTaipei Medical UniversityCollege of Medicine, Taipei Medical UniversityTaipei Medical UniversityUniv. Lille, CHU-Lille, Inserm, U1172, Lille Neuroscience & Cognition, FranceTaipei Medical UniversityHuman platelet lysates (HPLs), rich in various growth factors and cell growth-promoting molecules, encompass a new range of blood products that are being used for regenerative medicine, cell therapies, and tissue engineering. Well-characterized dedicated preparations, tailor-made to best fit specific therapeutic applications, are needed for optimal clinical efficacy and safety. Here, five types of HPL were prepared from the same platelet concentrates and extensively characterized to determine and compare their proteins, growth factors, cytokines, biochemical profiles, thrombin-generating capacities, thrombin-associated proteolytic activities, phospholipid-associated procoagulant potential, contents of extracellular vesicles expressing phosphatidylserine and tissue factor, and antioxidative properties. Our results revealed that all five HPL preparations contained detectable supraphysiological levels, in the ca. 0.1 ~ 350-ng/ml range, of all growth factors assessed, except insulin-like growth factor-1 detected only in HPL containing plasma. There were significant differences observed among these HPLs in total protein content, fibrinogen, complement components C3 and C4, albumin, and immunoglobulin G, and, most importantly, in their functional coagulant and procoagulant activities and antioxidative capacities. Our data revealed that the biochemical and functional properties of HPL preparations greatly vary depending upon their mode of production, with potential impacts on the safety and efficacy for certain clinical indications. Modes of preparation of HPLs should be carefully designed, and the product properties carefully evaluated based on the intended therapeutic use to ensure optimal clinical outcomes.http://dx.doi.org/10.1080/09537104.2020.1849603cell therapyhplplatelet lysateregenerative medicine |
| spellingShingle | Liling Delila Yu-Wen Wu Ouada Nebie Rifa Widyaningrum Ming-Li Chou David Devos Thierry Burnouf Extensive characterization of the composition and functional activities of five preparations of human platelet lysates for dedicated clinical uses Platelets cell therapy hpl platelet lysate regenerative medicine |
| title | Extensive characterization of the composition and functional activities of five preparations of human platelet lysates for dedicated clinical uses |
| title_full | Extensive characterization of the composition and functional activities of five preparations of human platelet lysates for dedicated clinical uses |
| title_fullStr | Extensive characterization of the composition and functional activities of five preparations of human platelet lysates for dedicated clinical uses |
| title_full_unstemmed | Extensive characterization of the composition and functional activities of five preparations of human platelet lysates for dedicated clinical uses |
| title_short | Extensive characterization of the composition and functional activities of five preparations of human platelet lysates for dedicated clinical uses |
| title_sort | extensive characterization of the composition and functional activities of five preparations of human platelet lysates for dedicated clinical uses |
| topic | cell therapy hpl platelet lysate regenerative medicine |
| url | http://dx.doi.org/10.1080/09537104.2020.1849603 |
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