A Multicenter Blinded Analysis Indicates No Association between Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and either Xenotropic Murine Leukemia Virus-Related Virus or Polytropic Murine Leukemia Virus
ABSTRACT The disabling disorder known as chronic fatigue syndrome or myalgic encephalomyelitis (CFS/ME) has been linked in two independent studies to infection with xenotropic murine leukemia virus-related virus (XMRV) and polytropic murine leukemia virus (pMLV). Although the associations were not c...
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2012-11-01
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| Series: | mBio |
| Online Access: | https://journals.asm.org/doi/10.1128/mBio.00266-12 |
| _version_ | 1818568730257391616 |
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| author | Harvey J. Alter Judy A. Mikovits William M. Switzer Francis W. Ruscetti Shyh-Ching Lo Nancy Klimas Anthony L. Komaroff Jose G. Montoya Lucinda Bateman Susan Levine Daniel Peterson Bruce Levin Maureen R. Hanson Afia Genfi Meera Bhat HaoQiang Zheng Richard Wang Bingjie Li Guo-Chiuan Hung Li Ling Lee Stephen Sameroff Walid Heneine John Coffin Mady Hornig W. Ian Lipkin |
| author_facet | Harvey J. Alter Judy A. Mikovits William M. Switzer Francis W. Ruscetti Shyh-Ching Lo Nancy Klimas Anthony L. Komaroff Jose G. Montoya Lucinda Bateman Susan Levine Daniel Peterson Bruce Levin Maureen R. Hanson Afia Genfi Meera Bhat HaoQiang Zheng Richard Wang Bingjie Li Guo-Chiuan Hung Li Ling Lee Stephen Sameroff Walid Heneine John Coffin Mady Hornig W. Ian Lipkin |
| author_sort | Harvey J. Alter |
| collection | DOAJ |
| description | ABSTRACT The disabling disorder known as chronic fatigue syndrome or myalgic encephalomyelitis (CFS/ME) has been linked in two independent studies to infection with xenotropic murine leukemia virus-related virus (XMRV) and polytropic murine leukemia virus (pMLV). Although the associations were not confirmed in subsequent studies by other investigators, patients continue to question the consensus of the scientific community in rejecting the validity of the association. Here we report blinded analysis of peripheral blood from a rigorously characterized, geographically diverse population of 147 patients with CFS/ME and 146 healthy subjects by the investigators describing the original association. This analysis reveals no evidence of either XMRV or pMLV infection. IMPORTANCE Chronic fatigue syndrome/myalgic encephalomyelitis has an estimated prevalence of 42/10,000 in the United States, with annual direct medical costs of $7 billion. Here, the original investigators who found XMRV and pMLV (polytropic murine leukemia virus) in blood of subjects with this disorder report that this association is not confirmed in a blinded analysis of samples from rigorously characterized subjects. The increasing frequency with which molecular methods are used for pathogen discovery poses new challenges to public health and support of science. It is imperative that strategies be developed to rapidly and coherently address discoveries so that they can be carried forward for translation to clinical medicine or abandoned to focus resource investment more productively. Our study provides a paradigm for pathogen dediscovery that may be helpful to others working in this field. |
| first_indexed | 2024-12-14T06:38:56Z |
| format | Article |
| id | doaj.art-48262110e27541f9a37a03c1055947ac |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-12-14T06:38:56Z |
| publishDate | 2012-11-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj.art-48262110e27541f9a37a03c1055947ac2022-12-21T23:13:16ZengAmerican Society for MicrobiologymBio2150-75112012-11-013510.1128/mBio.00266-12A Multicenter Blinded Analysis Indicates No Association between Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and either Xenotropic Murine Leukemia Virus-Related Virus or Polytropic Murine Leukemia VirusHarvey J. Alter0Judy A. Mikovits1William M. Switzer2Francis W. Ruscetti3Shyh-Ching Lo4Nancy Klimas5Anthony L. Komaroff6Jose G. Montoya7Lucinda Bateman8Susan Levine9Daniel Peterson10Bruce Levin11Maureen R. Hanson12Afia Genfi13Meera Bhat14HaoQiang Zheng15Richard Wang16Bingjie Li17Guo-Chiuan Hung18Li Ling Lee19Stephen Sameroff20Walid Heneine21John Coffin22Mady Hornig23W. Ian Lipkin24Department of Transfusion Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland, USAMikovits Consulting, Oxnard, California, USADivision of HIV/AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USACancer and Inflammation Program, Frederick National Laboratory for Cancer Research, Frederick, Maryland, USATissue Safety Laboratory, Office of Cellular, Tissue and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USANova Southeastern University, Fort Lauderdale Florida, USABrigham and Women’s Hospital, Boston, Massachusetts, USAInfectious Disease Clinic, Stanford University, Palo Alto, California, USAFatigue Consultation Clinic, Salt Lake City, Utah, USALevine Clinic, New York, New York, USASimmaron Research Institute, Incline Village, Nevada, USADepartment of Biostatistics, Columbia University, New York, New York, USADepartment of Molecular Biology and Genetics, Cornell University, Ithaca, New York, USACenter for Infection and Immunity, Columbia University, New York, New York, USACenter for Infection and Immunity, Columbia University, New York, New York, USADivision of HIV/AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USADepartment of Transfusion Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland, USATissue Safety Laboratory, Office of Cellular, Tissue and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USATissue Safety Laboratory, Office of Cellular, Tissue and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USADepartment of Molecular Biology and Genetics, Cornell University, Ithaca, New York, USACenter for Infection and Immunity, Columbia University, New York, New York, USADivision of HIV/AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USADepartment of Molecular Biology and Microbiology, Tufts University, Boston, Massachusetts, USACenter for Infection and Immunity, Columbia University, New York, New York, USACenter for Infection and Immunity, Columbia University, New York, New York, USAABSTRACT The disabling disorder known as chronic fatigue syndrome or myalgic encephalomyelitis (CFS/ME) has been linked in two independent studies to infection with xenotropic murine leukemia virus-related virus (XMRV) and polytropic murine leukemia virus (pMLV). Although the associations were not confirmed in subsequent studies by other investigators, patients continue to question the consensus of the scientific community in rejecting the validity of the association. Here we report blinded analysis of peripheral blood from a rigorously characterized, geographically diverse population of 147 patients with CFS/ME and 146 healthy subjects by the investigators describing the original association. This analysis reveals no evidence of either XMRV or pMLV infection. IMPORTANCE Chronic fatigue syndrome/myalgic encephalomyelitis has an estimated prevalence of 42/10,000 in the United States, with annual direct medical costs of $7 billion. Here, the original investigators who found XMRV and pMLV (polytropic murine leukemia virus) in blood of subjects with this disorder report that this association is not confirmed in a blinded analysis of samples from rigorously characterized subjects. The increasing frequency with which molecular methods are used for pathogen discovery poses new challenges to public health and support of science. It is imperative that strategies be developed to rapidly and coherently address discoveries so that they can be carried forward for translation to clinical medicine or abandoned to focus resource investment more productively. Our study provides a paradigm for pathogen dediscovery that may be helpful to others working in this field.https://journals.asm.org/doi/10.1128/mBio.00266-12 |
| spellingShingle | Harvey J. Alter Judy A. Mikovits William M. Switzer Francis W. Ruscetti Shyh-Ching Lo Nancy Klimas Anthony L. Komaroff Jose G. Montoya Lucinda Bateman Susan Levine Daniel Peterson Bruce Levin Maureen R. Hanson Afia Genfi Meera Bhat HaoQiang Zheng Richard Wang Bingjie Li Guo-Chiuan Hung Li Ling Lee Stephen Sameroff Walid Heneine John Coffin Mady Hornig W. Ian Lipkin A Multicenter Blinded Analysis Indicates No Association between Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and either Xenotropic Murine Leukemia Virus-Related Virus or Polytropic Murine Leukemia Virus mBio |
| title | A Multicenter Blinded Analysis Indicates No Association between Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and either Xenotropic Murine Leukemia Virus-Related Virus or Polytropic Murine Leukemia Virus |
| title_full | A Multicenter Blinded Analysis Indicates No Association between Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and either Xenotropic Murine Leukemia Virus-Related Virus or Polytropic Murine Leukemia Virus |
| title_fullStr | A Multicenter Blinded Analysis Indicates No Association between Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and either Xenotropic Murine Leukemia Virus-Related Virus or Polytropic Murine Leukemia Virus |
| title_full_unstemmed | A Multicenter Blinded Analysis Indicates No Association between Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and either Xenotropic Murine Leukemia Virus-Related Virus or Polytropic Murine Leukemia Virus |
| title_short | A Multicenter Blinded Analysis Indicates No Association between Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and either Xenotropic Murine Leukemia Virus-Related Virus or Polytropic Murine Leukemia Virus |
| title_sort | multicenter blinded analysis indicates no association between chronic fatigue syndrome myalgic encephalomyelitis and either xenotropic murine leukemia virus related virus or polytropic murine leukemia virus |
| url | https://journals.asm.org/doi/10.1128/mBio.00266-12 |
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