The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition
Abstract Background The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development. Gut microbiota has a vital role to host animal. However, little was known about the r...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | BMC Microbiology |
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| Online Access: | http://link.springer.com/article/10.1186/s12866-018-1246-0 |
| _version_ | 1818128972011012096 |
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| author | Jin Wei Xianwu Guo Han Liu Yuanyuan Chen Weimin Wang |
| author_facet | Jin Wei Xianwu Guo Han Liu Yuanyuan Chen Weimin Wang |
| author_sort | Jin Wei |
| collection | DOAJ |
| description | Abstract Background The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development. Gut microbiota has a vital role to host animal. However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content. Results In this study, 186,328 high-quality reads from nine 16S rRNA libraries were obtained using the Illumina MiSeq PE300 platform. The valid sequences were classified into 388 Operational Taxonomic Units, and a total of 223 genera, belonging to 20 phyla, were identified. The clustering result of gut bacterial communities is consistently related to the clustering result of intestinal content compositions. Proteobacteria and Firmicutes constitute the ‘core’ gut microbiota of blunt snout bream. Cetobacterium and Rhizobium were identified as microbiological markers of gut microbiota at zooplankton-based diet stages and diet transition stages, respectively. Moreover, thirteen potential cellulose-degrading bacteria were detected in our study. The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit. Conclusions This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit. This work allows us to better understand the relationship among gut microbiota, nutrition metabolism and feeding habits in vertebrate. Further, our study provides a reference for future studies investigating the metabolic adaption of herbivorous fish to shift to a vegetarian diet during their life history. |
| first_indexed | 2024-12-11T07:41:44Z |
| format | Article |
| id | doaj.art-48270a0a07be4e378a5f276d4ba701e7 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-11T07:41:44Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-48270a0a07be4e378a5f276d4ba701e72022-12-22T01:15:33ZengBMCBMC Microbiology1471-21802018-09-0118111410.1186/s12866-018-1246-0The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transitionJin Wei0Xianwu Guo1Han Liu2Yuanyuan Chen3Weimin Wang4College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural UniversityLab of Biotecnología Genómica, Centro de Biotecnología Genómica, Instituto de Politécnico NacionalCollege of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural UniversityCollege of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural UniversityCollege of Fisheries, Huazhong Agricultural UniversityAbstract Background The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development. Gut microbiota has a vital role to host animal. However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content. Results In this study, 186,328 high-quality reads from nine 16S rRNA libraries were obtained using the Illumina MiSeq PE300 platform. The valid sequences were classified into 388 Operational Taxonomic Units, and a total of 223 genera, belonging to 20 phyla, were identified. The clustering result of gut bacterial communities is consistently related to the clustering result of intestinal content compositions. Proteobacteria and Firmicutes constitute the ‘core’ gut microbiota of blunt snout bream. Cetobacterium and Rhizobium were identified as microbiological markers of gut microbiota at zooplankton-based diet stages and diet transition stages, respectively. Moreover, thirteen potential cellulose-degrading bacteria were detected in our study. The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit. Conclusions This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit. This work allows us to better understand the relationship among gut microbiota, nutrition metabolism and feeding habits in vertebrate. Further, our study provides a reference for future studies investigating the metabolic adaption of herbivorous fish to shift to a vegetarian diet during their life history.http://link.springer.com/article/10.1186/s12866-018-1246-0Blunt snout breamGut microbiotaFeeding habit transitionCore microbiomeEnzyme activityCellulose-degrading bacteria |
| spellingShingle | Jin Wei Xianwu Guo Han Liu Yuanyuan Chen Weimin Wang The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition BMC Microbiology Blunt snout bream Gut microbiota Feeding habit transition Core microbiome Enzyme activity Cellulose-degrading bacteria |
| title | The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition |
| title_full | The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition |
| title_fullStr | The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition |
| title_full_unstemmed | The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition |
| title_short | The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition |
| title_sort | variation profile of intestinal microbiota in blunt snout bream megalobrama amblycephala during feeding habit transition |
| topic | Blunt snout bream Gut microbiota Feeding habit transition Core microbiome Enzyme activity Cellulose-degrading bacteria |
| url | http://link.springer.com/article/10.1186/s12866-018-1246-0 |
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