The Cysteine-Rich Repeat Protein TaCRR1 Participates in Defense against Both <i>Rhizoctonia</i> <i>cerealis</i> and <i>Bipolaris sorokiniana</i> in Wheat
The domain of unknown function 26 (DUF26), harboring a conserved cysteine-rich motif (C-X8-C-X2-C), is unique to land plants. Several cysteine-rich repeat proteins (CRRs), belonging to DUF26-containing proteins, have been implicated in the defense against fungal pathogens in ginkgo, cotton, and maiz...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/16/5698 |
| Summary: | The domain of unknown function 26 (DUF26), harboring a conserved cysteine-rich motif (C-X8-C-X2-C), is unique to land plants. Several cysteine-rich repeat proteins (CRRs), belonging to DUF26-containing proteins, have been implicated in the defense against fungal pathogens in ginkgo, cotton, and maize. However, little is known about the functional roles of CRRs in the important staple crop wheat (<i>Triticum aestivum</i>). In this study, we identified a wheat CRR-encoding gene <i>TaCRR1</i> through transcriptomic analysis, and dissected the defense role of <i>TaCRR1</i> against the soil-borne fungi <i>Rhizoctonia cerealis</i> and <i>Bipolaris sorokiniana</i>, causal pathogens of destructive wheat diseases. <i>TaCRR1</i> transcription was up-regulated in wheat towards <i>B. Sorokiniana</i> or <i>R. cerealis</i> infection. The deduced TaCRR1 protein contained a signal peptide and two DUF26 domains. Heterologously-expressed TaCRR1 protein markedly inhibited the mycelia growth of <i>B. sorokiniana</i> and <i>R. cerealis</i>. Furthermore, the silencing of <i>TaCRR1</i> both impaired host resistance to <i>B. sorokiniana</i> and <i>R</i>. <i>cerealis</i> and repressed the expression of several pathogenesis-related genes in wheat. These results suggest that the TaCRR1 positively participated in wheat defense against both <i>B. sorokiniana</i> and <i>R. cerealis</i> through its antifungal activity and modulating expression of pathogenesis-related genes. Thus, <i>TaCRR1</i> is a candidate gene for improving wheat resistance to <i>B. sorokiniana</i> and <i>R. cerealis</i>. |
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| ISSN: | 1661-6596 1422-0067 |