Investigation on mineralization performance and spore germination conditions of calcium carbonate mineralizing bacteria
Microbial induced calcium carbonate precipitation (MICP) provides a theoretical basis for repairing underground rocks. The optimal germination conditions and mineralization activities of Bacillus pasteurii with crack repair function were investigated. The growth and mineralization properties of micr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2022-01-01
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| Series: | Materials Research Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/2053-1591/ac7709 |
| _version_ | 1797746326629253120 |
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| author | Yingying Hu Weitao Liu Qingtao Zhang Xuelong Hu |
| author_facet | Yingying Hu Weitao Liu Qingtao Zhang Xuelong Hu |
| author_sort | Yingying Hu |
| collection | DOAJ |
| description | Microbial induced calcium carbonate precipitation (MICP) provides a theoretical basis for repairing underground rocks. The optimal germination conditions and mineralization activities of Bacillus pasteurii with crack repair function were investigated. The growth and mineralization properties of microorganisms in different environments were explored by measuring the urease activity and calcium carbonate production of bacteria, and the influencing factors of bacterial biomass and urease activity were analyzed. The microscopic morphology of bacterial mineralization products was analyzed by SEM, XRD and TG-DSC test. The influencing factors of spore germination rate were studied by measuring the OD value of spore germination. The results showed that under the conditions of pH 7.3, urea concentration 0.25–0.5 mol l ^−1 , and calcium ion concentration 0.4–0.6 mol l ^−1 , the mineralization activity of B. pasteurii was the strongest, and the generated mineralization product was a stable calcite crystal. The optimum concentration of germination agent and inorganic salt were 10 mmol l ^−1 and 200 mmol l ^−1 , respectively. |
| first_indexed | 2024-03-12T15:35:14Z |
| format | Article |
| id | doaj.art-482f82c993f740e78d58f0ff988d9a7d |
| institution | Directory Open Access Journal |
| issn | 2053-1591 |
| language | English |
| last_indexed | 2024-03-12T15:35:14Z |
| publishDate | 2022-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Materials Research Express |
| spelling | doaj.art-482f82c993f740e78d58f0ff988d9a7d2023-08-09T16:14:17ZengIOP PublishingMaterials Research Express2053-15912022-01-019606540310.1088/2053-1591/ac7709Investigation on mineralization performance and spore germination conditions of calcium carbonate mineralizing bacteriaYingying Hu0Weitao Liu1Qingtao Zhang2Xuelong Hu3https://orcid.org/0000-0003-1783-0935College of Mining and Safety Engineering, Shandong University of Science and Technology , Qingdao, People’s Republic of China; Department of Chemical Engineering and Safety, Bin Zhou University , Bin Zhou, People’s Republic of China; Key Laboratory of Safety and High-efficiency Coal Mining, Ministry of Education (Anhui University of Science and Technology) , Huainan, People’s Republic of China; Department of Safety Engineering, College of Mining and Safety Engineering, Shandong University of Science and Technology. 579 Qianwangang Road , Economic & Technical Development Zone, Qingdao Shandong Province, 266590, People’s Republic of China; Key Laboratory of Safety and High-efficiency Coal Mining, Ministry of Education (Anhui University of Science and Technology) , 168 Taifeng Street, Huainan City, Anhui Province, 232001, People’s Republic of China; Department of Chemical Engineering and Safety, Bin Zhou University , 391 Huanghe 5th Road, Bincheng Zone, Binzhou Shandong Province, 256600 People’s Republic of ChinaCollege of Mining and Safety Engineering, Shandong University of Science and Technology , Qingdao, People’s Republic of China; Department of Safety Engineering, College of Mining and Safety Engineering, Shandong University of Science and Technology. 579 Qianwangang Road , Economic & Technical Development Zone, Qingdao Shandong Province, 266590, People’s Republic of ChinaDepartment of Architectural Engineering, Bin Zhou University , Bin Zhou, People’s Republic of China; Department of Architectural Engineering, Bin Zhou University , 391 Huanghe 5th Road, Bincheng Zone, Binzhou Shandong Province, 256600, People’s Republic of ChinaKey Laboratory of Safety and High-efficiency Coal Mining, Ministry of Education (Anhui University of Science and Technology) , Huainan, People’s Republic of China; Key Laboratory of Safety and High-efficiency Coal Mining, Ministry of Education (Anhui University of Science and Technology) , 168 Taifeng Street, Huainan City, Anhui Province, 232001, People’s Republic of ChinaMicrobial induced calcium carbonate precipitation (MICP) provides a theoretical basis for repairing underground rocks. The optimal germination conditions and mineralization activities of Bacillus pasteurii with crack repair function were investigated. The growth and mineralization properties of microorganisms in different environments were explored by measuring the urease activity and calcium carbonate production of bacteria, and the influencing factors of bacterial biomass and urease activity were analyzed. The microscopic morphology of bacterial mineralization products was analyzed by SEM, XRD and TG-DSC test. The influencing factors of spore germination rate were studied by measuring the OD value of spore germination. The results showed that under the conditions of pH 7.3, urea concentration 0.25–0.5 mol l ^−1 , and calcium ion concentration 0.4–0.6 mol l ^−1 , the mineralization activity of B. pasteurii was the strongest, and the generated mineralization product was a stable calcite crystal. The optimum concentration of germination agent and inorganic salt were 10 mmol l ^−1 and 200 mmol l ^−1 , respectively.https://doi.org/10.1088/2053-1591/ac7709bacillus pasteuriicrack-healinggerminationsporulation rateMICP |
| spellingShingle | Yingying Hu Weitao Liu Qingtao Zhang Xuelong Hu Investigation on mineralization performance and spore germination conditions of calcium carbonate mineralizing bacteria Materials Research Express bacillus pasteurii crack-healing germination sporulation rate MICP |
| title | Investigation on mineralization performance and spore germination conditions of calcium carbonate mineralizing bacteria |
| title_full | Investigation on mineralization performance and spore germination conditions of calcium carbonate mineralizing bacteria |
| title_fullStr | Investigation on mineralization performance and spore germination conditions of calcium carbonate mineralizing bacteria |
| title_full_unstemmed | Investigation on mineralization performance and spore germination conditions of calcium carbonate mineralizing bacteria |
| title_short | Investigation on mineralization performance and spore germination conditions of calcium carbonate mineralizing bacteria |
| title_sort | investigation on mineralization performance and spore germination conditions of calcium carbonate mineralizing bacteria |
| topic | bacillus pasteurii crack-healing germination sporulation rate MICP |
| url | https://doi.org/10.1088/2053-1591/ac7709 |
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