Lymph Nodes Draining Infections Investigated by PET and Immunohistochemistry in a Juvenile Porcine Model
Background: [<sup>18</sup>F]FDG and [<sup>11</sup>C]methionine accumulate in lymph nodes draining <i>S. aureus</i> -infected foci. The lymph nodes were characterized by weight, [<sup>11</sup>C]methionine- and [<sup>18</sup>F]FDG-positron em...
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MDPI AG
2022-04-01
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| Online Access: | https://www.mdpi.com/1420-3049/27/9/2792 |
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| author | Pia Afzelius Malene Kjelin Morsing Ole Lerberg Nielsen Aage Kristian Olsen Alstrup Svend Borup Jensen Lars Jødal |
| author_facet | Pia Afzelius Malene Kjelin Morsing Ole Lerberg Nielsen Aage Kristian Olsen Alstrup Svend Borup Jensen Lars Jødal |
| author_sort | Pia Afzelius |
| collection | DOAJ |
| description | Background: [<sup>18</sup>F]FDG and [<sup>11</sup>C]methionine accumulate in lymph nodes draining <i>S. aureus</i> -infected foci. The lymph nodes were characterized by weight, [<sup>11</sup>C]methionine- and [<sup>18</sup>F]FDG-positron emissions tomography (PET)/computed tomography (CT), and immunohistochemical (IHC)-staining. Methods: 20 pigs inoculated with <i>S. aureus</i> into the right femoral artery were PET/CT-scanned with [<sup>18</sup>F]FDG, and nine of the pigs were additionally scanned with [<sup>11</sup>C]methionine. Mammary, medial iliac, and popliteal lymph nodes from the left and right hind limbs were weighed. IHC-staining for calculations of area fractions of Ki-67, L1, and IL-8 positive cells was done in mammary and popliteal lymph nodes from the nine pigs. Results: The pigs developed one to six osteomyelitis foci. Some pigs developed contiguous infections of peri-osseous tissue and inoculation-site abscesses. Weights of mammary and medial iliac lymph nodes and their [<sup>18</sup>F]FDG maximum Standardized Uptake Values (SUV<sub>FDGmax</sub>) showed a significant increase in the inoculated limb compared to the left limb. Popliteal lymph node weight and their FDG uptake did not differ significantly between hind limbs. Area fractions of Ki-67 and IL-8 in the right mammary lymph nodes and SUV<sub>Metmax</sub> in the right popliteal lymph nodes were significantly increased compared with the left side. Conclusion: The PET-tracers [<sup>18</sup>F]FDG and [<sup>11</sup>C]methionine, and the IHC- markers Ki-67 and IL-8, but not L1, showed increased values in lymph nodes draining soft tissues infected with <i>S. aureus</i>. The increase in [<sup>11</sup>C]methionine may indicate a more acute lymph node response, whereas an increase in [<sup>18</sup>F]FDG may indicate a more chronic response. |
| first_indexed | 2024-03-10T03:54:31Z |
| format | Article |
| id | doaj.art-4830a3774e4741018609035c6fa5a099 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:54:31Z |
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| spelling | doaj.art-4830a3774e4741018609035c6fa5a0992023-11-23T08:49:37ZengMDPI AGMolecules1420-30492022-04-01279279210.3390/molecules27092792Lymph Nodes Draining Infections Investigated by PET and Immunohistochemistry in a Juvenile Porcine ModelPia Afzelius0Malene Kjelin Morsing1Ole Lerberg Nielsen2Aage Kristian Olsen Alstrup3Svend Borup Jensen4Lars Jødal5Department of Clinical Physiology and Nuclear Medicine Zealand Koege, University Hospital of Copenhagen, 4600 Copenhagen, DenmarkDepartment of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, DenmarkDepartment of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, DenmarkDepartment of Nuclear Medicine and PET, Aarhus University Hospital, Skejby, 8200 Aarhus, DenmarkDepartment of Nuclear Medicine, Aalborg University Hospital, 9000 Aalborg, DenmarkDepartment of Nuclear Medicine, Aalborg University Hospital, 9000 Aalborg, DenmarkBackground: [<sup>18</sup>F]FDG and [<sup>11</sup>C]methionine accumulate in lymph nodes draining <i>S. aureus</i> -infected foci. The lymph nodes were characterized by weight, [<sup>11</sup>C]methionine- and [<sup>18</sup>F]FDG-positron emissions tomography (PET)/computed tomography (CT), and immunohistochemical (IHC)-staining. Methods: 20 pigs inoculated with <i>S. aureus</i> into the right femoral artery were PET/CT-scanned with [<sup>18</sup>F]FDG, and nine of the pigs were additionally scanned with [<sup>11</sup>C]methionine. Mammary, medial iliac, and popliteal lymph nodes from the left and right hind limbs were weighed. IHC-staining for calculations of area fractions of Ki-67, L1, and IL-8 positive cells was done in mammary and popliteal lymph nodes from the nine pigs. Results: The pigs developed one to six osteomyelitis foci. Some pigs developed contiguous infections of peri-osseous tissue and inoculation-site abscesses. Weights of mammary and medial iliac lymph nodes and their [<sup>18</sup>F]FDG maximum Standardized Uptake Values (SUV<sub>FDGmax</sub>) showed a significant increase in the inoculated limb compared to the left limb. Popliteal lymph node weight and their FDG uptake did not differ significantly between hind limbs. Area fractions of Ki-67 and IL-8 in the right mammary lymph nodes and SUV<sub>Metmax</sub> in the right popliteal lymph nodes were significantly increased compared with the left side. Conclusion: The PET-tracers [<sup>18</sup>F]FDG and [<sup>11</sup>C]methionine, and the IHC- markers Ki-67 and IL-8, but not L1, showed increased values in lymph nodes draining soft tissues infected with <i>S. aureus</i>. The increase in [<sup>11</sup>C]methionine may indicate a more acute lymph node response, whereas an increase in [<sup>18</sup>F]FDG may indicate a more chronic response.https://www.mdpi.com/1420-3049/27/9/2792PET[<sup>18</sup>F]FDG[<sup>11</sup>C]methioninelymph nodespig model<i>Staphylococcus aureus</i> |
| spellingShingle | Pia Afzelius Malene Kjelin Morsing Ole Lerberg Nielsen Aage Kristian Olsen Alstrup Svend Borup Jensen Lars Jødal Lymph Nodes Draining Infections Investigated by PET and Immunohistochemistry in a Juvenile Porcine Model Molecules PET [<sup>18</sup>F]FDG [<sup>11</sup>C]methionine lymph nodes pig model <i>Staphylococcus aureus</i> |
| title | Lymph Nodes Draining Infections Investigated by PET and Immunohistochemistry in a Juvenile Porcine Model |
| title_full | Lymph Nodes Draining Infections Investigated by PET and Immunohistochemistry in a Juvenile Porcine Model |
| title_fullStr | Lymph Nodes Draining Infections Investigated by PET and Immunohistochemistry in a Juvenile Porcine Model |
| title_full_unstemmed | Lymph Nodes Draining Infections Investigated by PET and Immunohistochemistry in a Juvenile Porcine Model |
| title_short | Lymph Nodes Draining Infections Investigated by PET and Immunohistochemistry in a Juvenile Porcine Model |
| title_sort | lymph nodes draining infections investigated by pet and immunohistochemistry in a juvenile porcine model |
| topic | PET [<sup>18</sup>F]FDG [<sup>11</sup>C]methionine lymph nodes pig model <i>Staphylococcus aureus</i> |
| url | https://www.mdpi.com/1420-3049/27/9/2792 |
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