Serotonin Mediates Depression of Aggression After Acute and Chronic Social Defeat Stress in a Model Insect
In all animals, losers of a conflict against a conspecific exhibit reduced aggressiveness, often coupled with depression-like symptoms, particularly after multiple defeats. While serotonin (5HT) is involved, discovering its natural role in aggression and depression has proven elusive. We show how 5H...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Behavioral Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fnbeh.2018.00233/full |
| _version_ | 1811203076086824960 |
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| author | Jan Rillich Paul A. Stevenson |
| author_facet | Jan Rillich Paul A. Stevenson |
| author_sort | Jan Rillich |
| collection | DOAJ |
| description | In all animals, losers of a conflict against a conspecific exhibit reduced aggressiveness, often coupled with depression-like symptoms, particularly after multiple defeats. While serotonin (5HT) is involved, discovering its natural role in aggression and depression has proven elusive. We show how 5HT influences aggression in male crickets, before, and after single and multiple defeats using serotonergic drugs, at dosages that had no obvious deleterious effect on general motility: the 5HT synthesis inhibitor alpha-methyltryptophan (AMTP), the 5HT2 receptor blocker ketanserin, methiothepin which blocks 5HT receptor subtypes other than 5HT2, 5HT's precursor 5-hydroxytryptophan (5HTP) and re-uptake inhibitor fluoxetine. Contrasting reports for other invertebrates, none of the drugs influenced aggression at the first encounter. However, the recovery of aggression after single defeat, which normally requires 3 h in crickets, was severely affected. Losers that received ketanserin or AMTP regained their aggressiveness sooner, whereas those that received fluoxetine, 5HTP, or methiothepin failed to recover within 3 h. Furthermore, compared to controls, which show long term aggressive depression 24 h after 6 defeats at 1 h intervals, crickets that received AMTP or ketanserin regained their full aggressiveness and were thus more resilient to chronic defeat stress. In contrast, 5HTP and fluoxetine treated crickets showed long term aggressive depression 24 h after only 2 defeats, and were thus more susceptible to defeat stress. We conclude that 5HT acts after social defeat via a 5HT2 like receptor to maintain depressed aggressiveness after defeat, and to promote the susceptibility to and establishment of long-term depression after chronic social defeat. It is known that the decision to flee and establishment of loser depression in crickets is controlled by nitric oxide (NO), whereas dopamine (DA), but not octopamine (OA) is necessary for recovery after defeat. Here we show that blocking NO synthesis, just like ketanserin, affords resilience to multiple defeat stress, whereas blocking DA receptors, but not OA receptors, increases susceptibility, just like fluoxetine. We discuss the possible interplay between 5HT, NO, DA, and OA in controlling aggression after defeat, as well as similarities and differences to findings in mammals and other invertebrate model systems. |
| first_indexed | 2024-04-12T02:48:31Z |
| format | Article |
| id | doaj.art-48332d1c7662492984d7eaccedfd173c |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-04-12T02:48:31Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-48332d1c7662492984d7eaccedfd173c2022-12-22T03:51:04ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532018-10-011210.3389/fnbeh.2018.00233414668Serotonin Mediates Depression of Aggression After Acute and Chronic Social Defeat Stress in a Model InsectJan RillichPaul A. StevensonIn all animals, losers of a conflict against a conspecific exhibit reduced aggressiveness, often coupled with depression-like symptoms, particularly after multiple defeats. While serotonin (5HT) is involved, discovering its natural role in aggression and depression has proven elusive. We show how 5HT influences aggression in male crickets, before, and after single and multiple defeats using serotonergic drugs, at dosages that had no obvious deleterious effect on general motility: the 5HT synthesis inhibitor alpha-methyltryptophan (AMTP), the 5HT2 receptor blocker ketanserin, methiothepin which blocks 5HT receptor subtypes other than 5HT2, 5HT's precursor 5-hydroxytryptophan (5HTP) and re-uptake inhibitor fluoxetine. Contrasting reports for other invertebrates, none of the drugs influenced aggression at the first encounter. However, the recovery of aggression after single defeat, which normally requires 3 h in crickets, was severely affected. Losers that received ketanserin or AMTP regained their aggressiveness sooner, whereas those that received fluoxetine, 5HTP, or methiothepin failed to recover within 3 h. Furthermore, compared to controls, which show long term aggressive depression 24 h after 6 defeats at 1 h intervals, crickets that received AMTP or ketanserin regained their full aggressiveness and were thus more resilient to chronic defeat stress. In contrast, 5HTP and fluoxetine treated crickets showed long term aggressive depression 24 h after only 2 defeats, and were thus more susceptible to defeat stress. We conclude that 5HT acts after social defeat via a 5HT2 like receptor to maintain depressed aggressiveness after defeat, and to promote the susceptibility to and establishment of long-term depression after chronic social defeat. It is known that the decision to flee and establishment of loser depression in crickets is controlled by nitric oxide (NO), whereas dopamine (DA), but not octopamine (OA) is necessary for recovery after defeat. Here we show that blocking NO synthesis, just like ketanserin, affords resilience to multiple defeat stress, whereas blocking DA receptors, but not OA receptors, increases susceptibility, just like fluoxetine. We discuss the possible interplay between 5HT, NO, DA, and OA in controlling aggression after defeat, as well as similarities and differences to findings in mammals and other invertebrate model systems.https://www.frontiersin.org/article/10.3389/fnbeh.2018.00233/fullaggressionagonistic behaviorsubjugationloser effect5HTnitric oxide |
| spellingShingle | Jan Rillich Paul A. Stevenson Serotonin Mediates Depression of Aggression After Acute and Chronic Social Defeat Stress in a Model Insect Frontiers in Behavioral Neuroscience aggression agonistic behavior subjugation loser effect 5HT nitric oxide |
| title | Serotonin Mediates Depression of Aggression After Acute and Chronic Social Defeat Stress in a Model Insect |
| title_full | Serotonin Mediates Depression of Aggression After Acute and Chronic Social Defeat Stress in a Model Insect |
| title_fullStr | Serotonin Mediates Depression of Aggression After Acute and Chronic Social Defeat Stress in a Model Insect |
| title_full_unstemmed | Serotonin Mediates Depression of Aggression After Acute and Chronic Social Defeat Stress in a Model Insect |
| title_short | Serotonin Mediates Depression of Aggression After Acute and Chronic Social Defeat Stress in a Model Insect |
| title_sort | serotonin mediates depression of aggression after acute and chronic social defeat stress in a model insect |
| topic | aggression agonistic behavior subjugation loser effect 5HT nitric oxide |
| url | https://www.frontiersin.org/article/10.3389/fnbeh.2018.00233/full |
| work_keys_str_mv | AT janrillich serotoninmediatesdepressionofaggressionafteracuteandchronicsocialdefeatstressinamodelinsect AT paulastevenson serotoninmediatesdepressionofaggressionafteracuteandchronicsocialdefeatstressinamodelinsect |