Expression and Significance of MyD88 in Patients With Gastric Cardia Cancer in a High-Incidence Area of China
Background: Gastric cardia cancer (GCC) arises in the area of the stomach adjoining the esophageal–gastric junction and has unique risk factors. It was suggested that the involvement of Helicobacter pylori is associated with GCC from high-risk population. Myeloid differentiation factor 88 (MyD88) is...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2020.00559/full |
| _version_ | 1819087573441576960 |
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| author | Jingyao Chen Di Xia Muming Xu Ruibing Su Wenting Lin Dan Guo Guangcan Chen Shuhui Liu |
| author_facet | Jingyao Chen Di Xia Muming Xu Ruibing Su Wenting Lin Dan Guo Guangcan Chen Shuhui Liu |
| author_sort | Jingyao Chen |
| collection | DOAJ |
| description | Background: Gastric cardia cancer (GCC) arises in the area of the stomach adjoining the esophageal–gastric junction and has unique risk factors. It was suggested that the involvement of Helicobacter pylori is associated with GCC from high-risk population. Myeloid differentiation factor 88 (MyD88) is a crucial adaptor molecule in Toll-like signaling pathway recognizing H. pylori. Its role in GCC has not been elucidated yet. In this study, our purpose is to investigate the expression and significance of MyD88 in GCC tissue.Methods: Expression of MyD88 and nuclear factor κB (NF-κB) p105/p50 and infection of H. pylori were detected by immunohistochemistry in gastric cardia tissue. The correlation of MyD88 expression to NF-κB p105/p50 expression, H. pylori infection, and clinicopathologic characteristics in gastric cardia tissue was analyzed. The involvement of MyD88 in patient prognosis was also analyzed.Results: Our data showed that the expression of MyD88 elevated from normal mucosa to inflammation (p = 0.071). The expression of MyD88 was enhanced in GCC tissues by contrast to non-malignant cardia mucosa (p = 0.025). What's more, overexpression of MyD88 was detected in intestinal-type adenocarcinoma with inflammation. Patients with high MyD88 staining revealed a better differentiation (p = 0.02). MyD88 also positively correlated with NF-κB p105/p50 expression (p = 0.012) in cancer tissue. Expression of MyD88 was increased but not significantly in biopsies with H. pylori infection compared with non-infected biopsies. Multivariate analyses revealed lymph node metastasis but not MyD88 expression was an independent predictor for patient survival.Conclusion: These findings provide pathological evidence that upregulating MyD88 and inducing inflammation might be involved in gastric cardia carcinogenesis in high-risk population. MyD88 plays a role in gastric cardia carcinogenesis with NF-κB pathway activation. Higher MyD88 expression is not a major prognostic determinant in GCC, but it may relate to the tumor cell differentiation. |
| first_indexed | 2024-12-21T21:38:18Z |
| format | Article |
| id | doaj.art-48333e089b70453aa61a6a0fa9f30655 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-21T21:38:18Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-48333e089b70453aa61a6a0fa9f306552022-12-21T18:49:26ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-05-011010.3389/fonc.2020.00559528018Expression and Significance of MyD88 in Patients With Gastric Cardia Cancer in a High-Incidence Area of ChinaJingyao Chen0Di Xia1Muming Xu2Ruibing Su3Wenting Lin4Dan Guo5Guangcan Chen6Shuhui Liu7Department of Pathology, Shantou University Medical College, Shantou, ChinaDepartment of Pathology, Shantou University Medical College, Shantou, ChinaDepartment of Abdominal Surgery, The Tumor Hospital of Shantou University Medical College, Shantou, ChinaDepartment of Pathology, Shantou University Medical College, Shantou, ChinaDepartment of Pathology, Shantou University Medical College, Shantou, ChinaDepartment of Pathology, Shantou University Medical College, Shantou, ChinaDepartment of Gastrointestinal Surgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, ChinaDepartment of Pathology, Shantou University Medical College, Shantou, ChinaBackground: Gastric cardia cancer (GCC) arises in the area of the stomach adjoining the esophageal–gastric junction and has unique risk factors. It was suggested that the involvement of Helicobacter pylori is associated with GCC from high-risk population. Myeloid differentiation factor 88 (MyD88) is a crucial adaptor molecule in Toll-like signaling pathway recognizing H. pylori. Its role in GCC has not been elucidated yet. In this study, our purpose is to investigate the expression and significance of MyD88 in GCC tissue.Methods: Expression of MyD88 and nuclear factor κB (NF-κB) p105/p50 and infection of H. pylori were detected by immunohistochemistry in gastric cardia tissue. The correlation of MyD88 expression to NF-κB p105/p50 expression, H. pylori infection, and clinicopathologic characteristics in gastric cardia tissue was analyzed. The involvement of MyD88 in patient prognosis was also analyzed.Results: Our data showed that the expression of MyD88 elevated from normal mucosa to inflammation (p = 0.071). The expression of MyD88 was enhanced in GCC tissues by contrast to non-malignant cardia mucosa (p = 0.025). What's more, overexpression of MyD88 was detected in intestinal-type adenocarcinoma with inflammation. Patients with high MyD88 staining revealed a better differentiation (p = 0.02). MyD88 also positively correlated with NF-κB p105/p50 expression (p = 0.012) in cancer tissue. Expression of MyD88 was increased but not significantly in biopsies with H. pylori infection compared with non-infected biopsies. Multivariate analyses revealed lymph node metastasis but not MyD88 expression was an independent predictor for patient survival.Conclusion: These findings provide pathological evidence that upregulating MyD88 and inducing inflammation might be involved in gastric cardia carcinogenesis in high-risk population. MyD88 plays a role in gastric cardia carcinogenesis with NF-κB pathway activation. Higher MyD88 expression is not a major prognostic determinant in GCC, but it may relate to the tumor cell differentiation.https://www.frontiersin.org/article/10.3389/fonc.2020.00559/fullMyD88Helicobacter pylorigastric cardia cancercancer and inflammationprognosis |
| spellingShingle | Jingyao Chen Di Xia Muming Xu Ruibing Su Wenting Lin Dan Guo Guangcan Chen Shuhui Liu Expression and Significance of MyD88 in Patients With Gastric Cardia Cancer in a High-Incidence Area of China Frontiers in Oncology MyD88 Helicobacter pylori gastric cardia cancer cancer and inflammation prognosis |
| title | Expression and Significance of MyD88 in Patients With Gastric Cardia Cancer in a High-Incidence Area of China |
| title_full | Expression and Significance of MyD88 in Patients With Gastric Cardia Cancer in a High-Incidence Area of China |
| title_fullStr | Expression and Significance of MyD88 in Patients With Gastric Cardia Cancer in a High-Incidence Area of China |
| title_full_unstemmed | Expression and Significance of MyD88 in Patients With Gastric Cardia Cancer in a High-Incidence Area of China |
| title_short | Expression and Significance of MyD88 in Patients With Gastric Cardia Cancer in a High-Incidence Area of China |
| title_sort | expression and significance of myd88 in patients with gastric cardia cancer in a high incidence area of china |
| topic | MyD88 Helicobacter pylori gastric cardia cancer cancer and inflammation prognosis |
| url | https://www.frontiersin.org/article/10.3389/fonc.2020.00559/full |
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