Gilvimarinus xylanilyticus sp. nov., a novel 1,3-xylanase-secreting bacterium isolated from a marine green alga
1,3-xylan, an important organic carbon in the ocean, is peculiar to marine algae. 1,3-xylanase-secreting bacteria and their extracellular 1,3-xylanases play pivotal roles in the degradation and biomass conversion of 1,3-xylan. However, only a few 1,3-xylanase-secreting bacteria and 1,3-xylanases hav...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1006116/full |
| _version_ | 1811235526183747584 |
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| author | Yan-Jiao Zhang Hai-Ning Sun Hai-Ning Sun Ting-Ting Xu Dian-Li Zhao Chun-Mei Yu Yi Zhang Xi-Ying Zhang Xiu-Lan Chen Xiu-Lan Chen Yu-Qiang Zhang Fang Zhao |
| author_facet | Yan-Jiao Zhang Hai-Ning Sun Hai-Ning Sun Ting-Ting Xu Dian-Li Zhao Chun-Mei Yu Yi Zhang Xi-Ying Zhang Xiu-Lan Chen Xiu-Lan Chen Yu-Qiang Zhang Fang Zhao |
| author_sort | Yan-Jiao Zhang |
| collection | DOAJ |
| description | 1,3-xylan, an important organic carbon in the ocean, is peculiar to marine algae. 1,3-xylanase-secreting bacteria and their extracellular 1,3-xylanases play pivotal roles in the degradation and biomass conversion of 1,3-xylan. However, only a few 1,3-xylanase-secreting bacteria and 1,3-xylanases have been reported. Here, we identified a novel marine bacterium capable of secreting 1,3-xylanases, designated as strain HB14T. Phylogenetic analysis revealed that strain HB14T clustered tightly with known species of the genus Gilvimarinus, showing the highest 16S rRNA gene sequence similarity (97.7%) with the type strain of Gilvimarinus chinensis. Based on phylogenetic, genomic, chemotaxonomic and phenotypic studies, strain HB14T was classified as a representative of a novel species in the genus Gilvimarinus, for which the name Gilvimarinus xylanilyticus sp. nov. was proposed. The type strain is HB14T (=CCTCC AB 2022109T = KCTC 92379T). Four 1,3-xylanases secreted by strain HB14T were identified based on genome and secretome analyses, and the two (Xyn65 and Xyn80) with relatively higher abundance in secretome were successfully expressed in Escherichia coli and biochemically characterized. They showed the highest activity at pH 6.0–7.0 and 40°C and released mainly 1,3-xylobiose and 1,3-xylotriose from 1,3-xylan. These data suggest that strain HB14T acts as a player in marine 1,3-xylan degradation and recycling and that its extracellular 1,3-xylanases may have a good potential in 1,3-xylooligosaccharides preparation. |
| first_indexed | 2024-04-12T11:53:35Z |
| format | Article |
| id | doaj.art-483383ed357f415484ab87ea12d7bf3c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T11:53:35Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-483383ed357f415484ab87ea12d7bf3c2022-12-22T03:34:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.10061161006116Gilvimarinus xylanilyticus sp. nov., a novel 1,3-xylanase-secreting bacterium isolated from a marine green algaYan-Jiao Zhang0Hai-Ning Sun1Hai-Ning Sun2Ting-Ting Xu3Dian-Li Zhao4Chun-Mei Yu5Yi Zhang6Xi-Ying Zhang7Xiu-Lan Chen8Xiu-Lan Chen9Yu-Qiang Zhang10Fang Zhao11Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, ChinaCollege of Marine Life Sciences & Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, ChinaCollege of Marine Life Sciences & Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, ChinaCollege of Marine Life Sciences & Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, China1,3-xylan, an important organic carbon in the ocean, is peculiar to marine algae. 1,3-xylanase-secreting bacteria and their extracellular 1,3-xylanases play pivotal roles in the degradation and biomass conversion of 1,3-xylan. However, only a few 1,3-xylanase-secreting bacteria and 1,3-xylanases have been reported. Here, we identified a novel marine bacterium capable of secreting 1,3-xylanases, designated as strain HB14T. Phylogenetic analysis revealed that strain HB14T clustered tightly with known species of the genus Gilvimarinus, showing the highest 16S rRNA gene sequence similarity (97.7%) with the type strain of Gilvimarinus chinensis. Based on phylogenetic, genomic, chemotaxonomic and phenotypic studies, strain HB14T was classified as a representative of a novel species in the genus Gilvimarinus, for which the name Gilvimarinus xylanilyticus sp. nov. was proposed. The type strain is HB14T (=CCTCC AB 2022109T = KCTC 92379T). Four 1,3-xylanases secreted by strain HB14T were identified based on genome and secretome analyses, and the two (Xyn65 and Xyn80) with relatively higher abundance in secretome were successfully expressed in Escherichia coli and biochemically characterized. They showed the highest activity at pH 6.0–7.0 and 40°C and released mainly 1,3-xylobiose and 1,3-xylotriose from 1,3-xylan. These data suggest that strain HB14T acts as a player in marine 1,3-xylan degradation and recycling and that its extracellular 1,3-xylanases may have a good potential in 1,3-xylooligosaccharides preparation.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1006116/fullGilvimarinuspolyphasic taxonomysp. nov.1,3-xylanase-secreting bacteria1,3-xylanases |
| spellingShingle | Yan-Jiao Zhang Hai-Ning Sun Hai-Ning Sun Ting-Ting Xu Dian-Li Zhao Chun-Mei Yu Yi Zhang Xi-Ying Zhang Xiu-Lan Chen Xiu-Lan Chen Yu-Qiang Zhang Fang Zhao Gilvimarinus xylanilyticus sp. nov., a novel 1,3-xylanase-secreting bacterium isolated from a marine green alga Frontiers in Microbiology Gilvimarinus polyphasic taxonomy sp. nov. 1,3-xylanase-secreting bacteria 1,3-xylanases |
| title | Gilvimarinus xylanilyticus sp. nov., a novel 1,3-xylanase-secreting bacterium isolated from a marine green alga |
| title_full | Gilvimarinus xylanilyticus sp. nov., a novel 1,3-xylanase-secreting bacterium isolated from a marine green alga |
| title_fullStr | Gilvimarinus xylanilyticus sp. nov., a novel 1,3-xylanase-secreting bacterium isolated from a marine green alga |
| title_full_unstemmed | Gilvimarinus xylanilyticus sp. nov., a novel 1,3-xylanase-secreting bacterium isolated from a marine green alga |
| title_short | Gilvimarinus xylanilyticus sp. nov., a novel 1,3-xylanase-secreting bacterium isolated from a marine green alga |
| title_sort | gilvimarinus xylanilyticus sp nov a novel 1 3 xylanase secreting bacterium isolated from a marine green alga |
| topic | Gilvimarinus polyphasic taxonomy sp. nov. 1,3-xylanase-secreting bacteria 1,3-xylanases |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1006116/full |
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