Antibiotic Treatment Reduced the Gut Microbiota Diversity, Prolonged the Larval Development Period and Lessened Adult Fecundity of <i>Grapholita molesta</i> (Lepidoptera: Tortricidae)
<i>Grapholita molesta</i>, the oriental fruit moth, is a serious pest of fruit trees with host transfer characteristics worldwide. The gut microbiota, which plays a crucial part in insect physiology and ecology, can be influenced by many elements, such as antibiotics, temperature, diet,...
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MDPI AG
2022-09-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/9/838 |
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| author | Xuan Zhang Xing Wang Zikun Guo Xueying Liu Ping Wang Xiangqun Yuan Yiping Li |
| author_facet | Xuan Zhang Xing Wang Zikun Guo Xueying Liu Ping Wang Xiangqun Yuan Yiping Li |
| author_sort | Xuan Zhang |
| collection | DOAJ |
| description | <i>Grapholita molesta</i>, the oriental fruit moth, is a serious pest of fruit trees with host transfer characteristics worldwide. The gut microbiota, which plays a crucial part in insect physiology and ecology, can be influenced by many elements, such as antibiotics, temperature, diet, and species. However, the effects of antibiotics on <i>G. molesta</i> gut microbiota are still unclear. In this study, we selected five common antibiotic agents to test the inhibition of <i>G. molesta</i> gut microbiota, and found ciprofloxacin shown the best antibacterial activity. After feeding 1 μg/mL of ciprofloxacin, the relative abundance of Actinobacteria and Cyanobacteria decreased significantly, while that of Firmicutes and Bacteroidetes increased. PICRUSt2 analysis indicated that most functional prediction categories were enriched in the <i>G. molesta</i> gut, including amino acid transport and metabolism, translation, ribosomal structure and biogenesis, carbohydrate transport and metabolism, transcription, cell wall/membrane/envelope biogenesis, and energy production and conversion. Finally, ciprofloxacin feeding significantly affected larval growth, development, and reproduction, resulting in prolonged larval development duration, shortened adult longevity, and significantly decreased single female oviposition and egg hatchability. In addition, we isolated and purified some culturable bacteria belonging to Proteobacteria, Firmicutes, Actinobacteria, and cellulase-producing bacteria from the <i>G. molesta</i> midgut. In brief, our results demonstrate that antibiotics can have an impact on <i>G. molesta</i> gut bacterial communities, which is beneficial for host growth and development, as well as helping female adults produce more fertile eggs. These results will thus provide a theoretical reference for developing new green control technology for <i>G. molesta</i>. |
| first_indexed | 2024-03-09T23:38:50Z |
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| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T23:38:50Z |
| publishDate | 2022-09-01 |
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| series | Insects |
| spelling | doaj.art-4835729feef04a79a8f6db52a77ba9c82023-11-23T16:55:34ZengMDPI AGInsects2075-44502022-09-0113983810.3390/insects13090838Antibiotic Treatment Reduced the Gut Microbiota Diversity, Prolonged the Larval Development Period and Lessened Adult Fecundity of <i>Grapholita molesta</i> (Lepidoptera: Tortricidae)Xuan Zhang0Xing Wang1Zikun Guo2Xueying Liu3Ping Wang4Xiangqun Yuan5Yiping Li6Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaDepartment of Entomology, Cornell University, Ithaca, NY 14850, USAKey Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, China<i>Grapholita molesta</i>, the oriental fruit moth, is a serious pest of fruit trees with host transfer characteristics worldwide. The gut microbiota, which plays a crucial part in insect physiology and ecology, can be influenced by many elements, such as antibiotics, temperature, diet, and species. However, the effects of antibiotics on <i>G. molesta</i> gut microbiota are still unclear. In this study, we selected five common antibiotic agents to test the inhibition of <i>G. molesta</i> gut microbiota, and found ciprofloxacin shown the best antibacterial activity. After feeding 1 μg/mL of ciprofloxacin, the relative abundance of Actinobacteria and Cyanobacteria decreased significantly, while that of Firmicutes and Bacteroidetes increased. PICRUSt2 analysis indicated that most functional prediction categories were enriched in the <i>G. molesta</i> gut, including amino acid transport and metabolism, translation, ribosomal structure and biogenesis, carbohydrate transport and metabolism, transcription, cell wall/membrane/envelope biogenesis, and energy production and conversion. Finally, ciprofloxacin feeding significantly affected larval growth, development, and reproduction, resulting in prolonged larval development duration, shortened adult longevity, and significantly decreased single female oviposition and egg hatchability. In addition, we isolated and purified some culturable bacteria belonging to Proteobacteria, Firmicutes, Actinobacteria, and cellulase-producing bacteria from the <i>G. molesta</i> midgut. In brief, our results demonstrate that antibiotics can have an impact on <i>G. molesta</i> gut bacterial communities, which is beneficial for host growth and development, as well as helping female adults produce more fertile eggs. These results will thus provide a theoretical reference for developing new green control technology for <i>G. molesta</i>.https://www.mdpi.com/2075-4450/13/9/838oriental fruit moth16S rDNAciprofloxacinovipositionculturable bacteria |
| spellingShingle | Xuan Zhang Xing Wang Zikun Guo Xueying Liu Ping Wang Xiangqun Yuan Yiping Li Antibiotic Treatment Reduced the Gut Microbiota Diversity, Prolonged the Larval Development Period and Lessened Adult Fecundity of <i>Grapholita molesta</i> (Lepidoptera: Tortricidae) Insects oriental fruit moth 16S rDNA ciprofloxacin oviposition culturable bacteria |
| title | Antibiotic Treatment Reduced the Gut Microbiota Diversity, Prolonged the Larval Development Period and Lessened Adult Fecundity of <i>Grapholita molesta</i> (Lepidoptera: Tortricidae) |
| title_full | Antibiotic Treatment Reduced the Gut Microbiota Diversity, Prolonged the Larval Development Period and Lessened Adult Fecundity of <i>Grapholita molesta</i> (Lepidoptera: Tortricidae) |
| title_fullStr | Antibiotic Treatment Reduced the Gut Microbiota Diversity, Prolonged the Larval Development Period and Lessened Adult Fecundity of <i>Grapholita molesta</i> (Lepidoptera: Tortricidae) |
| title_full_unstemmed | Antibiotic Treatment Reduced the Gut Microbiota Diversity, Prolonged the Larval Development Period and Lessened Adult Fecundity of <i>Grapholita molesta</i> (Lepidoptera: Tortricidae) |
| title_short | Antibiotic Treatment Reduced the Gut Microbiota Diversity, Prolonged the Larval Development Period and Lessened Adult Fecundity of <i>Grapholita molesta</i> (Lepidoptera: Tortricidae) |
| title_sort | antibiotic treatment reduced the gut microbiota diversity prolonged the larval development period and lessened adult fecundity of i grapholita molesta i lepidoptera tortricidae |
| topic | oriental fruit moth 16S rDNA ciprofloxacin oviposition culturable bacteria |
| url | https://www.mdpi.com/2075-4450/13/9/838 |
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