Nuclear eIF4E Stimulates 3′-End Cleavage of Target RNAs
Summary: The eukaryotic translation initiation factor eIF4E is nuclear and cytoplasmic where it plays roles in export and translation of specific transcripts, respectively. When we were studying its mRNA export activity, we unexpectedly discovered that eIF4E drives the protein expression of elements...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-04-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124719304632 |
| _version_ | 1819174239967641600 |
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| author | Margaret Rose Davis Mildred Delaleau Katherine L.B. Borden |
| author_facet | Margaret Rose Davis Mildred Delaleau Katherine L.B. Borden |
| author_sort | Margaret Rose Davis |
| collection | DOAJ |
| description | Summary: The eukaryotic translation initiation factor eIF4E is nuclear and cytoplasmic where it plays roles in export and translation of specific transcripts, respectively. When we were studying its mRNA export activity, we unexpectedly discovered that eIF4E drives the protein expression of elements of the 3′-end core cleavage complex involved in cleavage and polyadenylation (CPA), including CPSF3, the enzyme responsible for cleavage, as well as its co-factors CPSF1, CPSF2, CPSF4, Symplekin, WDR33, and FIP1L1. Using multiple strategies, we demonstrate that eIF4E stimulates 3′-end cleavage of selected RNAs. eIF4E physically interacts with CPSF3, CPSF1, and uncleaved target RNA, suggesting it acts directly and indirectly on the pathway. Through these effects, eIF4E can generate better substrates for its mRNA export and translation activities. Thus, we identified an unanticipated function for eIF4E in 3′-end processing of specific target RNAs, and this function could potentially affect the expression of a broad range of oncoproteins. : Davis et al. demonstrate that the eukaryotic translation initiation factor eIF4E, which is usually associated with nuclear export and translation of specific transcripts, also acts in 3′-end processing of selected RNAs. Through these effects, eIF4E can generate better substrates for its export and translation activities and, thus, modulate the proteome. Keywords: nuclear RNA export, polyadenylation, translation |
| first_indexed | 2024-12-22T20:35:49Z |
| format | Article |
| id | doaj.art-48357669f435479387bb7fc6d5bf602e |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-22T20:35:49Z |
| publishDate | 2019-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-48357669f435479387bb7fc6d5bf602e2022-12-21T18:13:28ZengElsevierCell Reports2211-12472019-04-0127513971408.e4Nuclear eIF4E Stimulates 3′-End Cleavage of Target RNAsMargaret Rose Davis0Mildred Delaleau1Katherine L.B. Borden2Institute of Research in Immunology and Cancer (IRIC), Department of Pathology and Cell Biology, Université de Montréal, Pavillon Marcelle-Coutu, 2950 Chemin de Polytechnique, Montreal, QC H3T 1J4, CanadaInstitute of Research in Immunology and Cancer (IRIC), Department of Pathology and Cell Biology, Université de Montréal, Pavillon Marcelle-Coutu, 2950 Chemin de Polytechnique, Montreal, QC H3T 1J4, CanadaInstitute of Research in Immunology and Cancer (IRIC), Department of Pathology and Cell Biology, Université de Montréal, Pavillon Marcelle-Coutu, 2950 Chemin de Polytechnique, Montreal, QC H3T 1J4, Canada; Corresponding authorSummary: The eukaryotic translation initiation factor eIF4E is nuclear and cytoplasmic where it plays roles in export and translation of specific transcripts, respectively. When we were studying its mRNA export activity, we unexpectedly discovered that eIF4E drives the protein expression of elements of the 3′-end core cleavage complex involved in cleavage and polyadenylation (CPA), including CPSF3, the enzyme responsible for cleavage, as well as its co-factors CPSF1, CPSF2, CPSF4, Symplekin, WDR33, and FIP1L1. Using multiple strategies, we demonstrate that eIF4E stimulates 3′-end cleavage of selected RNAs. eIF4E physically interacts with CPSF3, CPSF1, and uncleaved target RNA, suggesting it acts directly and indirectly on the pathway. Through these effects, eIF4E can generate better substrates for its mRNA export and translation activities. Thus, we identified an unanticipated function for eIF4E in 3′-end processing of specific target RNAs, and this function could potentially affect the expression of a broad range of oncoproteins. : Davis et al. demonstrate that the eukaryotic translation initiation factor eIF4E, which is usually associated with nuclear export and translation of specific transcripts, also acts in 3′-end processing of selected RNAs. Through these effects, eIF4E can generate better substrates for its export and translation activities and, thus, modulate the proteome. Keywords: nuclear RNA export, polyadenylation, translationhttp://www.sciencedirect.com/science/article/pii/S2211124719304632 |
| spellingShingle | Margaret Rose Davis Mildred Delaleau Katherine L.B. Borden Nuclear eIF4E Stimulates 3′-End Cleavage of Target RNAs Cell Reports |
| title | Nuclear eIF4E Stimulates 3′-End Cleavage of Target RNAs |
| title_full | Nuclear eIF4E Stimulates 3′-End Cleavage of Target RNAs |
| title_fullStr | Nuclear eIF4E Stimulates 3′-End Cleavage of Target RNAs |
| title_full_unstemmed | Nuclear eIF4E Stimulates 3′-End Cleavage of Target RNAs |
| title_short | Nuclear eIF4E Stimulates 3′-End Cleavage of Target RNAs |
| title_sort | nuclear eif4e stimulates 3 end cleavage of target rnas |
| url | http://www.sciencedirect.com/science/article/pii/S2211124719304632 |
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