Progression of microstructural deterioration in load-bearing immobilization osteopenia.
<h4>Purpose</h4>Immobilization osteopenia is a major healthcare problem in clinical and social medicine. However, the mechanisms underlying this bone pathology caused by immobilization under load-bearing conditions are not yet fully understood. This study aimed to evaluate sequential cha...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0275439 |
| _version_ | 1797856347918696448 |
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| author | Hironobu Koseki Makoto Osaki Yuichiro Honda Shinya Sunagawa Chieko Imai Takayuki Shida Umi Matsumura Junya Sakamoto Iku Tomonaga Seiichi Yokoo Satoshi Mizukami Minoru Okita |
| author_facet | Hironobu Koseki Makoto Osaki Yuichiro Honda Shinya Sunagawa Chieko Imai Takayuki Shida Umi Matsumura Junya Sakamoto Iku Tomonaga Seiichi Yokoo Satoshi Mizukami Minoru Okita |
| author_sort | Hironobu Koseki |
| collection | DOAJ |
| description | <h4>Purpose</h4>Immobilization osteopenia is a major healthcare problem in clinical and social medicine. However, the mechanisms underlying this bone pathology caused by immobilization under load-bearing conditions are not yet fully understood. This study aimed to evaluate sequential changes to the three-dimensional microstructure of bone in load-bearing immobilization osteopenia using a fixed-limb rat model.<h4>Materials and method</h4>Eight-week-old specific-pathogen-free male Wistar rats were divided into an immobilized group and a control group (n = 60 each). Hind limbs in the immobilized group were fixed using orthopedic casts with fixation periods of 1, 2, 4, 8, and 12 weeks. Feeding and weight-bearing were freely permitted. Length of the right femur was measured after each fixation period and bone microstructure was analyzed by micro-computed tomography. The architectural parameters of cortical and cancellous bone were analyzed statistically.<h4>Results</h4>Femoral length was significantly shorter in the immobilized group than in the control group after 2 weeks. Total area and marrow area were significantly lower in the immobilized group than in the control group from 1 to 12 weeks. Cortical bone area, cortical thickness, and polar moment of inertia decreased significantly after 2 weeks. Some cancellous bone parameters showed osteoporotic changes at 2 weeks after immobilization and the gap with the control group widened as the fixation period extended (P < 0.05).<h4>Conclusion</h4>The present results indicate that load-bearing immobilization triggers early deterioration of microstructure in both cortical and cancellous bone after 2 weeks. |
| first_indexed | 2024-04-09T20:38:56Z |
| format | Article |
| id | doaj.art-483653e4982b4e62a502c180b410d39c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-09T20:38:56Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-483653e4982b4e62a502c180b410d39c2023-03-30T05:31:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011711e027543910.1371/journal.pone.0275439Progression of microstructural deterioration in load-bearing immobilization osteopenia.Hironobu KosekiMakoto OsakiYuichiro HondaShinya SunagawaChieko ImaiTakayuki ShidaUmi MatsumuraJunya SakamotoIku TomonagaSeiichi YokooSatoshi MizukamiMinoru Okita<h4>Purpose</h4>Immobilization osteopenia is a major healthcare problem in clinical and social medicine. However, the mechanisms underlying this bone pathology caused by immobilization under load-bearing conditions are not yet fully understood. This study aimed to evaluate sequential changes to the three-dimensional microstructure of bone in load-bearing immobilization osteopenia using a fixed-limb rat model.<h4>Materials and method</h4>Eight-week-old specific-pathogen-free male Wistar rats were divided into an immobilized group and a control group (n = 60 each). Hind limbs in the immobilized group were fixed using orthopedic casts with fixation periods of 1, 2, 4, 8, and 12 weeks. Feeding and weight-bearing were freely permitted. Length of the right femur was measured after each fixation period and bone microstructure was analyzed by micro-computed tomography. The architectural parameters of cortical and cancellous bone were analyzed statistically.<h4>Results</h4>Femoral length was significantly shorter in the immobilized group than in the control group after 2 weeks. Total area and marrow area were significantly lower in the immobilized group than in the control group from 1 to 12 weeks. Cortical bone area, cortical thickness, and polar moment of inertia decreased significantly after 2 weeks. Some cancellous bone parameters showed osteoporotic changes at 2 weeks after immobilization and the gap with the control group widened as the fixation period extended (P < 0.05).<h4>Conclusion</h4>The present results indicate that load-bearing immobilization triggers early deterioration of microstructure in both cortical and cancellous bone after 2 weeks.https://doi.org/10.1371/journal.pone.0275439 |
| spellingShingle | Hironobu Koseki Makoto Osaki Yuichiro Honda Shinya Sunagawa Chieko Imai Takayuki Shida Umi Matsumura Junya Sakamoto Iku Tomonaga Seiichi Yokoo Satoshi Mizukami Minoru Okita Progression of microstructural deterioration in load-bearing immobilization osteopenia. PLoS ONE |
| title | Progression of microstructural deterioration in load-bearing immobilization osteopenia. |
| title_full | Progression of microstructural deterioration in load-bearing immobilization osteopenia. |
| title_fullStr | Progression of microstructural deterioration in load-bearing immobilization osteopenia. |
| title_full_unstemmed | Progression of microstructural deterioration in load-bearing immobilization osteopenia. |
| title_short | Progression of microstructural deterioration in load-bearing immobilization osteopenia. |
| title_sort | progression of microstructural deterioration in load bearing immobilization osteopenia |
| url | https://doi.org/10.1371/journal.pone.0275439 |
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