Linking endogenous decay and sludge bulking in the microbial community to membrane fouling at sub-critical flux
This study examined membrane fouling and associated microbial taxa in a membrane bioreactor operating at a sub-critical flux condition using next-generation amplicon sequencing. The membrane was operated at a sub-critical flux, thus, fouling was not observed until endogenous decay. The observed foul...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Journal of Membrane Science Letters |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772421222000113 |
| _version_ | 1828554568885075968 |
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| author | Anh Q. Nguyen Luong N. Nguyen Md Abu Hasan Johir Huu Hao Ngo Long D. Nghiem |
| author_facet | Anh Q. Nguyen Luong N. Nguyen Md Abu Hasan Johir Huu Hao Ngo Long D. Nghiem |
| author_sort | Anh Q. Nguyen |
| collection | DOAJ |
| description | This study examined membrane fouling and associated microbial taxa in a membrane bioreactor operating at a sub-critical flux condition using next-generation amplicon sequencing. The membrane was operated at a sub-critical flux, thus, fouling was not observed until endogenous decay. The observed fouling could be attributed to endogenous decay which was driven by nutrient deficiency at high sludge age and low food-to-microorganisms ratio (decreasing from 0.15 to 0.09 gBOD/gMLVSS.d). Endogenous decay resulted in a sharp decrease of the number of species and evenness between different species (49.7 and 58.9% compared to the inoculum, respectively). The release of dissolved organic matters and cell debris from endogenous decay as well as the excessive growth of filamentous bacteria, e.g. Thiotrichales were the main contributors to membrane fouling. The relative abundance of Thiotrichales significantly correlated with TMP (Pearson R = 0.996, p-value <0.001), indicating this order's contribution to membrane fouling. Other dominant orders in the mixed liquor after endogenous decay such as Rhizobiales, Burkholderiales, Rhodospirillales and Myxococcales, Flavobacteriales can produce extracellular polymeric substances and aggravating membrane fouling. Fouling layers possess highly similar microbial composition with the mixed liquor, with some filamentous microbial orders, e.g. Corynebacteriales and Oligoflexales showing increased relative abundance by 6.83 and 5.64 folds, respectively. |
| first_indexed | 2024-12-12T05:38:09Z |
| format | Article |
| id | doaj.art-483800c8386d48a7b9a8a6cee0b50ecc |
| institution | Directory Open Access Journal |
| issn | 2772-4212 |
| language | English |
| last_indexed | 2024-12-12T05:38:09Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Membrane Science Letters |
| spelling | doaj.art-483800c8386d48a7b9a8a6cee0b50ecc2022-12-22T00:36:01ZengElsevierJournal of Membrane Science Letters2772-42122022-05-0121100023Linking endogenous decay and sludge bulking in the microbial community to membrane fouling at sub-critical fluxAnh Q. Nguyen0Luong N. Nguyen1Md Abu Hasan Johir2Huu Hao Ngo3Long D. Nghiem4Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo NSW 2007, AustraliaCentre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo NSW 2007, AustraliaCentre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo NSW 2007, AustraliaCentre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo NSW 2007, AustraliaCorresponding author.; Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo NSW 2007, AustraliaThis study examined membrane fouling and associated microbial taxa in a membrane bioreactor operating at a sub-critical flux condition using next-generation amplicon sequencing. The membrane was operated at a sub-critical flux, thus, fouling was not observed until endogenous decay. The observed fouling could be attributed to endogenous decay which was driven by nutrient deficiency at high sludge age and low food-to-microorganisms ratio (decreasing from 0.15 to 0.09 gBOD/gMLVSS.d). Endogenous decay resulted in a sharp decrease of the number of species and evenness between different species (49.7 and 58.9% compared to the inoculum, respectively). The release of dissolved organic matters and cell debris from endogenous decay as well as the excessive growth of filamentous bacteria, e.g. Thiotrichales were the main contributors to membrane fouling. The relative abundance of Thiotrichales significantly correlated with TMP (Pearson R = 0.996, p-value <0.001), indicating this order's contribution to membrane fouling. Other dominant orders in the mixed liquor after endogenous decay such as Rhizobiales, Burkholderiales, Rhodospirillales and Myxococcales, Flavobacteriales can produce extracellular polymeric substances and aggravating membrane fouling. Fouling layers possess highly similar microbial composition with the mixed liquor, with some filamentous microbial orders, e.g. Corynebacteriales and Oligoflexales showing increased relative abundance by 6.83 and 5.64 folds, respectively.http://www.sciencedirect.com/science/article/pii/S2772421222000113Membrane foulingMicrobial communityMembrane bioreactorsBiofouling layerSludge bulking |
| spellingShingle | Anh Q. Nguyen Luong N. Nguyen Md Abu Hasan Johir Huu Hao Ngo Long D. Nghiem Linking endogenous decay and sludge bulking in the microbial community to membrane fouling at sub-critical flux Journal of Membrane Science Letters Membrane fouling Microbial community Membrane bioreactors Biofouling layer Sludge bulking |
| title | Linking endogenous decay and sludge bulking in the microbial community to membrane fouling at sub-critical flux |
| title_full | Linking endogenous decay and sludge bulking in the microbial community to membrane fouling at sub-critical flux |
| title_fullStr | Linking endogenous decay and sludge bulking in the microbial community to membrane fouling at sub-critical flux |
| title_full_unstemmed | Linking endogenous decay and sludge bulking in the microbial community to membrane fouling at sub-critical flux |
| title_short | Linking endogenous decay and sludge bulking in the microbial community to membrane fouling at sub-critical flux |
| title_sort | linking endogenous decay and sludge bulking in the microbial community to membrane fouling at sub critical flux |
| topic | Membrane fouling Microbial community Membrane bioreactors Biofouling layer Sludge bulking |
| url | http://www.sciencedirect.com/science/article/pii/S2772421222000113 |
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