Study of genetic diversity using molecular markers in sunflower (Helianthus annuus)
Considering the significance of sunflower (Helianthus annuus L.) cultivation under diverse agro-climatic conditions, present study was done to recognize assorted parental lines utilizing RAPD and ISSR markers to lessen environmental effects or test blunders at Rajasthan College of Agriculture, MPUA...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2021-09-01
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| Series: | The Indian Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/115129 |
| _version_ | 1811164848259596288 |
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| author | RAJESH DHUTMAL SHIV RATAN MALOO A W MORE VIJAY SHARMA ANU VIVEK K SINGH |
| author_facet | RAJESH DHUTMAL SHIV RATAN MALOO A W MORE VIJAY SHARMA ANU VIVEK K SINGH |
| author_sort | RAJESH DHUTMAL |
| collection | DOAJ |
| description | Considering the significance of sunflower (Helianthus annuus L.) cultivation under diverse agro-climatic conditions, present study was done to recognize assorted parental lines utilizing RAPD and ISSR markers to lessen environmental effects or test blunders at Rajasthan College of Agriculture, MPUA & T, Udaipur, Rajasthan during 2016. In the present study, RAPD primers produced 334 polymorphic band and ISSR primers produced 160 polymorphic bands. RAPD primers detected more polymorphic loci (56.88%) than the ISSR primers (52.24%). The dendrograms constructed by RAPD, ISSR primers and pooled data were associated with each other, indicating the efficacy of both marker systems in genetic diversity study of sunflower. Based on RAPD, ISSR and pooled data analysis, the restorer line EC 623023, R 16 and seed parental line CMS 234B, PET 2-7-1B were found to be genetically most diverse and fall into different groups. Therefore, their genetic origin seems to be different and could be efficiently used in sunflower breeding programs. |
| first_indexed | 2024-04-10T15:27:52Z |
| format | Article |
| id | doaj.art-48399068135a4d7d86810859df1448bb |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-04-10T15:27:52Z |
| publishDate | 2021-09-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-48399068135a4d7d86810859df1448bb2023-02-14T07:57:49ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192021-09-0191710.56093/ijas.v91i7.115129Study of genetic diversity using molecular markers in sunflower (Helianthus annuus)RAJESH DHUTMAL0SHIV RATAN MALOO1A W MORE2VIJAY SHARMA3ANU4VIVEK K SINGH5Maharana Pratap University of Agriculture and Technology, Udaipur, Rajasthan 313 001, IndiaMaharana Pratap University of Agriculture and Technology, Udaipur, Rajasthan 313 001, IndiaSorghum Research Station, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbahni, MaharashtraBanda University of Agriculture and Technology, Banda, Uttar PradeshCCS Haryana Agricultural University, Hisar, HaryanaCCS Haryana Agricultural University, Hisar, HaryanaConsidering the significance of sunflower (Helianthus annuus L.) cultivation under diverse agro-climatic conditions, present study was done to recognize assorted parental lines utilizing RAPD and ISSR markers to lessen environmental effects or test blunders at Rajasthan College of Agriculture, MPUA & T, Udaipur, Rajasthan during 2016. In the present study, RAPD primers produced 334 polymorphic band and ISSR primers produced 160 polymorphic bands. RAPD primers detected more polymorphic loci (56.88%) than the ISSR primers (52.24%). The dendrograms constructed by RAPD, ISSR primers and pooled data were associated with each other, indicating the efficacy of both marker systems in genetic diversity study of sunflower. Based on RAPD, ISSR and pooled data analysis, the restorer line EC 623023, R 16 and seed parental line CMS 234B, PET 2-7-1B were found to be genetically most diverse and fall into different groups. Therefore, their genetic origin seems to be different and could be efficiently used in sunflower breeding programs.https://epubs.icar.org.in/index.php/IJAgS/article/view/115129Genetic diversityISSRRAPDSunflower |
| spellingShingle | RAJESH DHUTMAL SHIV RATAN MALOO A W MORE VIJAY SHARMA ANU VIVEK K SINGH Study of genetic diversity using molecular markers in sunflower (Helianthus annuus) The Indian Journal of Agricultural Sciences Genetic diversity ISSR RAPD Sunflower |
| title | Study of genetic diversity using molecular markers in sunflower (Helianthus annuus) |
| title_full | Study of genetic diversity using molecular markers in sunflower (Helianthus annuus) |
| title_fullStr | Study of genetic diversity using molecular markers in sunflower (Helianthus annuus) |
| title_full_unstemmed | Study of genetic diversity using molecular markers in sunflower (Helianthus annuus) |
| title_short | Study of genetic diversity using molecular markers in sunflower (Helianthus annuus) |
| title_sort | study of genetic diversity using molecular markers in sunflower helianthus annuus |
| topic | Genetic diversity ISSR RAPD Sunflower |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/115129 |
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