Silencing of the Ortholog of <i>DEFECTIVE IN ANTHER DEHISCENCE 1</i> Gene in the Woody Perennial <i>Jatropha curcas</i> Alters Flower and Fruit Development
DEFECTIVE IN ANTHER DEHISCENCE 1 (DAD1), a phospholipase A1, utilizes galactolipids (18:3) to generate α-linolenic acid (ALA) in the initial step of jasmonic acid (JA) biosynthesis in <i>Arabidopsis thaliana</i>. In this study, we isolated the <i>JcDAD1</i> gene, an ortholog...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/23/8923 |
| Summary: | DEFECTIVE IN ANTHER DEHISCENCE 1 (DAD1), a phospholipase A1, utilizes galactolipids (18:3) to generate α-linolenic acid (ALA) in the initial step of jasmonic acid (JA) biosynthesis in <i>Arabidopsis thaliana</i>. In this study, we isolated the <i>JcDAD1</i> gene, an ortholog of <i>Arabidopsis DAD1</i> in <i>Jatropha curcas</i>, and found that it is mainly expressed in the stems, roots, and male flowers of <i>Jatropha</i>. <i>JcDAD1</i>-RNAi transgenic plants with low endogenous jasmonate levels in inflorescences exhibited more and larger flowers, as well as a few abortive female flowers, although anther and pollen development were normal. In addition, fruit number was increased and the seed size, weight, and oil contents were reduced in the transgenic <i>Jatropha</i> plants. These results indicate that <i>JcDAD1</i> regulates the development of flowers and fruits through the JA biosynthesis pathway, but does not alter androecium development in <i>Jatropha</i>. These findings strengthen our understanding of the roles of JA and <i>DAD1</i> in the regulation of floral development in woody perennial plants. |
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| ISSN: | 1661-6596 1422-0067 |