Equine Immunoglobulin and Equine Neutralizing F(ab′)2 Protect Mice from West Nile Virus Infection
West Nile virus (WNV) is prevalent in Africa, Europe, the Middle East, West Asia, and North America, and causes epidemic encephalitis. To date, no effective therapy for WNV infection has been developed; therefore, there is urgent need to find an efficient method to prevent WNV disease. In this study...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-12-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/8/12/332 |
| _version_ | 1818172626916343808 |
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| author | Jiannan Cui Yongkun Zhao Hualei Wang Boning Qiu Zengguo Cao Qian Li Yanbo Zhang Feihu Yan Hongli Jin Tiecheng Wang Weiyang Sun Na Feng Yuwei Gao Jing Sun Yanqun Wang Stanley Perlman Jincun Zhao Songtao Yang Xianzhu Xia |
| author_facet | Jiannan Cui Yongkun Zhao Hualei Wang Boning Qiu Zengguo Cao Qian Li Yanbo Zhang Feihu Yan Hongli Jin Tiecheng Wang Weiyang Sun Na Feng Yuwei Gao Jing Sun Yanqun Wang Stanley Perlman Jincun Zhao Songtao Yang Xianzhu Xia |
| author_sort | Jiannan Cui |
| collection | DOAJ |
| description | West Nile virus (WNV) is prevalent in Africa, Europe, the Middle East, West Asia, and North America, and causes epidemic encephalitis. To date, no effective therapy for WNV infection has been developed; therefore, there is urgent need to find an efficient method to prevent WNV disease. In this study, we prepared and evaluated the protective efficacy of immune serum IgG and pepsin-digested F(ab′)2 fragments from horses immunized with the WNV virus-like particles (VLP) expressing the WNV M and E proteins. Immune equine F(ab′)2 fragments and immune horse sera efficiently neutralized WNV infection in tissue culture. The passive transfer of equine immune antibodies significantly accelerated the virus clearance in the spleens and brains of WNV infected mice, and reduced mortality. Thus, equine immunoglobulin or equine neutralizing F(ab′)2 passive immunotherapy is a potential strategy for the prophylactic or therapeutic treatment of patients infected with WNV. |
| first_indexed | 2024-12-11T19:15:37Z |
| format | Article |
| id | doaj.art-4840135b944043c29394556390e2e94f |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-11T19:15:37Z |
| publishDate | 2016-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-4840135b944043c29394556390e2e94f2022-12-22T00:53:40ZengMDPI AGViruses1999-49152016-12-0181233210.3390/v8120332v8120332Equine Immunoglobulin and Equine Neutralizing F(ab′)2 Protect Mice from West Nile Virus InfectionJiannan Cui0Yongkun Zhao1Hualei Wang2Boning Qiu3Zengguo Cao4Qian Li5Yanbo Zhang6Feihu Yan7Hongli Jin8Tiecheng Wang9Weiyang Sun10Na Feng11Yuwei Gao12Jing Sun13Yanqun Wang14Stanley Perlman15Jincun Zhao16Songtao Yang17Xianzhu Xia18College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, ChinaState Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, ChinaState Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, ChinaDepartment of Microbiology, University of Iowa, Iowa City, IA 52242, USAState Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, ChinaWest Nile virus (WNV) is prevalent in Africa, Europe, the Middle East, West Asia, and North America, and causes epidemic encephalitis. To date, no effective therapy for WNV infection has been developed; therefore, there is urgent need to find an efficient method to prevent WNV disease. In this study, we prepared and evaluated the protective efficacy of immune serum IgG and pepsin-digested F(ab′)2 fragments from horses immunized with the WNV virus-like particles (VLP) expressing the WNV M and E proteins. Immune equine F(ab′)2 fragments and immune horse sera efficiently neutralized WNV infection in tissue culture. The passive transfer of equine immune antibodies significantly accelerated the virus clearance in the spleens and brains of WNV infected mice, and reduced mortality. Thus, equine immunoglobulin or equine neutralizing F(ab′)2 passive immunotherapy is a potential strategy for the prophylactic or therapeutic treatment of patients infected with WNV.http://www.mdpi.com/1999-4915/8/12/332West Nile virusequine immunoglobulinF(ab′)2 fragmentsmice |
| spellingShingle | Jiannan Cui Yongkun Zhao Hualei Wang Boning Qiu Zengguo Cao Qian Li Yanbo Zhang Feihu Yan Hongli Jin Tiecheng Wang Weiyang Sun Na Feng Yuwei Gao Jing Sun Yanqun Wang Stanley Perlman Jincun Zhao Songtao Yang Xianzhu Xia Equine Immunoglobulin and Equine Neutralizing F(ab′)2 Protect Mice from West Nile Virus Infection Viruses West Nile virus equine immunoglobulin F(ab′)2 fragments mice |
| title | Equine Immunoglobulin and Equine Neutralizing F(ab′)2 Protect Mice from West Nile Virus Infection |
| title_full | Equine Immunoglobulin and Equine Neutralizing F(ab′)2 Protect Mice from West Nile Virus Infection |
| title_fullStr | Equine Immunoglobulin and Equine Neutralizing F(ab′)2 Protect Mice from West Nile Virus Infection |
| title_full_unstemmed | Equine Immunoglobulin and Equine Neutralizing F(ab′)2 Protect Mice from West Nile Virus Infection |
| title_short | Equine Immunoglobulin and Equine Neutralizing F(ab′)2 Protect Mice from West Nile Virus Infection |
| title_sort | equine immunoglobulin and equine neutralizing f ab 2 protect mice from west nile virus infection |
| topic | West Nile virus equine immunoglobulin F(ab′)2 fragments mice |
| url | http://www.mdpi.com/1999-4915/8/12/332 |
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