BUBR1 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma
Chromosomal instability (CIN) plays a key role in the carcinogenesis of several human cancers and can be related to the deregulation of core components of the spindle assembly checkpoint (SAC) including BUBR1 protein kinase. These proteins have been related to tumor development and poor survival rat...
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/2076-2615/12/22/3082 |
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| author | Leonor Delgado Luís Monteiro Patrícia Silva Hassan Bousbaa Fernanda Garcez João Silva Paula Brilhante-Simões Isabel Pires Justina Prada |
| author_facet | Leonor Delgado Luís Monteiro Patrícia Silva Hassan Bousbaa Fernanda Garcez João Silva Paula Brilhante-Simões Isabel Pires Justina Prada |
| author_sort | Leonor Delgado |
| collection | DOAJ |
| description | Chromosomal instability (CIN) plays a key role in the carcinogenesis of several human cancers and can be related to the deregulation of core components of the spindle assembly checkpoint (SAC) including BUBR1 protein kinase. These proteins have been related to tumor development and poor survival rates in human patients with oral squamous cell carcinoma (OSCC). To investigate the expression of the SAC proteins BUBR1, BUB3 and SPINDLY and also Ki-67 in canine OSCC, we performed an immunohistochemical evaluation in 60 canine OSCCs and compared them with clinical and pathological variables. BUBR1, Ki-67, BUB3 and SPINDLY protein expressions were detected in all cases and classified as with a high-expression extent score in 31 (51.7%) cases for BUBR1, 33 (58.9%) cases for BUB3 and 28 (50.9%) cases for SPINDLY. Ki-67 high expression was observed in 14 (25%) cases. An independent prognostic value for BUBR1 was found, where high BUBR1 expression was associated with lower survival (<i>p</i> = 0.012). These results indicate that BUBR1 expression is an independent prognostic factor in these tumors, suggesting the potential use for clinical applications as a prognostic biomarker and also as a pharmacological target in canine OSCC. |
| first_indexed | 2024-03-09T18:32:06Z |
| format | Article |
| id | doaj.art-4843452acb144a63a1b84cd2d2d8c025 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T18:32:06Z |
| publishDate | 2022-11-01 |
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| spelling | doaj.art-4843452acb144a63a1b84cd2d2d8c0252023-11-24T07:28:00ZengMDPI AGAnimals2076-26152022-11-011222308210.3390/ani12223082BUBR1 as a Prognostic Biomarker in Canine Oral Squamous Cell CarcinomaLeonor Delgado0Luís Monteiro1Patrícia Silva2Hassan Bousbaa3Fernanda Garcez4João Silva5Paula Brilhante-Simões6Isabel Pires7Justina Prada8UNIPRO—Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences—CESPU (IUCS-CESPU), 4585-116 Gandra, PortugalUNIPRO—Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences—CESPU (IUCS-CESPU), 4585-116 Gandra, PortugalUNIPRO—Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences—CESPU (IUCS-CESPU), 4585-116 Gandra, PortugalUNIPRO—Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences—CESPU (IUCS-CESPU), 4585-116 Gandra, PortugalUNIPRO—Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences—CESPU (IUCS-CESPU), 4585-116 Gandra, PortugalUNIPRO—Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences—CESPU (IUCS-CESPU), 4585-116 Gandra, PortugalPathology Department, INNO Serviços Especializados em Veterinária, 4710-503 Braga, PortugalDepartment of Veterinary Science of the University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalDepartment of Veterinary Science of the University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalChromosomal instability (CIN) plays a key role in the carcinogenesis of several human cancers and can be related to the deregulation of core components of the spindle assembly checkpoint (SAC) including BUBR1 protein kinase. These proteins have been related to tumor development and poor survival rates in human patients with oral squamous cell carcinoma (OSCC). To investigate the expression of the SAC proteins BUBR1, BUB3 and SPINDLY and also Ki-67 in canine OSCC, we performed an immunohistochemical evaluation in 60 canine OSCCs and compared them with clinical and pathological variables. BUBR1, Ki-67, BUB3 and SPINDLY protein expressions were detected in all cases and classified as with a high-expression extent score in 31 (51.7%) cases for BUBR1, 33 (58.9%) cases for BUB3 and 28 (50.9%) cases for SPINDLY. Ki-67 high expression was observed in 14 (25%) cases. An independent prognostic value for BUBR1 was found, where high BUBR1 expression was associated with lower survival (<i>p</i> = 0.012). These results indicate that BUBR1 expression is an independent prognostic factor in these tumors, suggesting the potential use for clinical applications as a prognostic biomarker and also as a pharmacological target in canine OSCC.https://www.mdpi.com/2076-2615/12/22/3082oral cancerBUBR1BUB3SPINDLYKi-67immunohistochemistry |
| spellingShingle | Leonor Delgado Luís Monteiro Patrícia Silva Hassan Bousbaa Fernanda Garcez João Silva Paula Brilhante-Simões Isabel Pires Justina Prada BUBR1 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma Animals oral cancer BUBR1 BUB3 SPINDLY Ki-67 immunohistochemistry |
| title | BUBR1 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma |
| title_full | BUBR1 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma |
| title_fullStr | BUBR1 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma |
| title_full_unstemmed | BUBR1 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma |
| title_short | BUBR1 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma |
| title_sort | bubr1 as a prognostic biomarker in canine oral squamous cell carcinoma |
| topic | oral cancer BUBR1 BUB3 SPINDLY Ki-67 immunohistochemistry |
| url | https://www.mdpi.com/2076-2615/12/22/3082 |
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