Biological Activity of Extracts of Red and Yellow Fruits of <i>Cornus mas</i> L.—An In Vitro Evaluation of Antioxidant Activity, Inhibitory Activity against α-Glucosidase, Acetylcholinesterase, and Binding Capacity to Human Serum Albumin
Although extracts are broadly used in order to support the treatment of numerous diseases, only in a limited number of cases is the process of applying and establishing their mechanisms of action scientifically analyzed. Fruits of Cornelian cherry are an abundant source of iridoids, anthocyanins, fl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/7/2244 |
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| author | Olha Dzydzan Iryna Brodyak Paulina Strugała-Danak Angelika Strach Alicja Z. Kucharska Janina Gabrielska Natalia Sybirna |
| author_facet | Olha Dzydzan Iryna Brodyak Paulina Strugała-Danak Angelika Strach Alicja Z. Kucharska Janina Gabrielska Natalia Sybirna |
| author_sort | Olha Dzydzan |
| collection | DOAJ |
| description | Although extracts are broadly used in order to support the treatment of numerous diseases, only in a limited number of cases is the process of applying and establishing their mechanisms of action scientifically analyzed. Fruits of Cornelian cherry are an abundant source of iridoids, anthocyanins, flavonols and phenolic acids. The aim of the present study was to evaluate the in vitro bioactivity of red and yellow Cornelian cherry fruits’ extracts. The biological potential of extracts, in a broad sense, involved antioxidant activity in relation to phosphatidylcholine liposomes, inhibitory ability against α-glucosidase and acetylcholinesterase enzymes, as well as interactions with human serum albumin. Studies showed that both extracts were more effective in protecting liposome membranes against free radicals produced by AAPH in an aqueous environment due to the fact that they can be better eliminated by the hydrophilic components of the extracts than those produced by UVB radiation. Extracts exhibited inhibitory activity against acetylcholinesterase and α-glucosidase, wherein loganic acid extract showed noncompetitive inhibition of the enzyme. Moreover, extracts binded to albumin mainly through hydrogen bonds and van der Waals forces. Taken together, red and yellow cherry fruits’ extracts exhibit diverse biological properties and can be exploited as a source of natural therapeutic agents. |
| first_indexed | 2024-03-09T11:35:47Z |
| format | Article |
| id | doaj.art-4844a270b773445f9ebc4ec98756374f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T11:35:47Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4844a270b773445f9ebc4ec98756374f2023-11-30T23:41:33ZengMDPI AGMolecules1420-30492022-03-01277224410.3390/molecules27072244Biological Activity of Extracts of Red and Yellow Fruits of <i>Cornus mas</i> L.—An In Vitro Evaluation of Antioxidant Activity, Inhibitory Activity against α-Glucosidase, Acetylcholinesterase, and Binding Capacity to Human Serum AlbuminOlha Dzydzan0Iryna Brodyak1Paulina Strugała-Danak2Angelika Strach3Alicja Z. Kucharska4Janina Gabrielska5Natalia Sybirna6Department of Biochemistry, Ivan Franko National University of Lviv, 4 Hrushevskyi St., 79005 Lviv, UkraineDepartment of Biochemistry, Ivan Franko National University of Lviv, 4 Hrushevskyi St., 79005 Lviv, UkraineDepartment of Physics and Biophysics, Wrocław University of Environmental and Life Sciences, C. K. Norwida 25, 50-375 Wrocław, PolandDepartment of Physics and Biophysics, Wrocław University of Environmental and Life Sciences, C. K. Norwida 25, 50-375 Wrocław, PolandDepartment of Fruit, Vegetable and Plant Nutraceutical Technology, Wrocław University of Environmental and Life Sciences, J. Chełmońskiego 37/41, 51-630 Wrocław, PolandDepartment of Physics and Biophysics, Wrocław University of Environmental and Life Sciences, C. K. Norwida 25, 50-375 Wrocław, PolandDepartment of Biochemistry, Ivan Franko National University of Lviv, 4 Hrushevskyi St., 79005 Lviv, UkraineAlthough extracts are broadly used in order to support the treatment of numerous diseases, only in a limited number of cases is the process of applying and establishing their mechanisms of action scientifically analyzed. Fruits of Cornelian cherry are an abundant source of iridoids, anthocyanins, flavonols and phenolic acids. The aim of the present study was to evaluate the in vitro bioactivity of red and yellow Cornelian cherry fruits’ extracts. The biological potential of extracts, in a broad sense, involved antioxidant activity in relation to phosphatidylcholine liposomes, inhibitory ability against α-glucosidase and acetylcholinesterase enzymes, as well as interactions with human serum albumin. Studies showed that both extracts were more effective in protecting liposome membranes against free radicals produced by AAPH in an aqueous environment due to the fact that they can be better eliminated by the hydrophilic components of the extracts than those produced by UVB radiation. Extracts exhibited inhibitory activity against acetylcholinesterase and α-glucosidase, wherein loganic acid extract showed noncompetitive inhibition of the enzyme. Moreover, extracts binded to albumin mainly through hydrogen bonds and van der Waals forces. Taken together, red and yellow cherry fruits’ extracts exhibit diverse biological properties and can be exploited as a source of natural therapeutic agents.https://www.mdpi.com/1420-3049/27/7/2244<i>Cornus mas</i> L.α-glucosidaseacetylcholinesteraseenzyme inhibitionalbuminantioxidant activity |
| spellingShingle | Olha Dzydzan Iryna Brodyak Paulina Strugała-Danak Angelika Strach Alicja Z. Kucharska Janina Gabrielska Natalia Sybirna Biological Activity of Extracts of Red and Yellow Fruits of <i>Cornus mas</i> L.—An In Vitro Evaluation of Antioxidant Activity, Inhibitory Activity against α-Glucosidase, Acetylcholinesterase, and Binding Capacity to Human Serum Albumin Molecules <i>Cornus mas</i> L. α-glucosidase acetylcholinesterase enzyme inhibition albumin antioxidant activity |
| title | Biological Activity of Extracts of Red and Yellow Fruits of <i>Cornus mas</i> L.—An In Vitro Evaluation of Antioxidant Activity, Inhibitory Activity against α-Glucosidase, Acetylcholinesterase, and Binding Capacity to Human Serum Albumin |
| title_full | Biological Activity of Extracts of Red and Yellow Fruits of <i>Cornus mas</i> L.—An In Vitro Evaluation of Antioxidant Activity, Inhibitory Activity against α-Glucosidase, Acetylcholinesterase, and Binding Capacity to Human Serum Albumin |
| title_fullStr | Biological Activity of Extracts of Red and Yellow Fruits of <i>Cornus mas</i> L.—An In Vitro Evaluation of Antioxidant Activity, Inhibitory Activity against α-Glucosidase, Acetylcholinesterase, and Binding Capacity to Human Serum Albumin |
| title_full_unstemmed | Biological Activity of Extracts of Red and Yellow Fruits of <i>Cornus mas</i> L.—An In Vitro Evaluation of Antioxidant Activity, Inhibitory Activity against α-Glucosidase, Acetylcholinesterase, and Binding Capacity to Human Serum Albumin |
| title_short | Biological Activity of Extracts of Red and Yellow Fruits of <i>Cornus mas</i> L.—An In Vitro Evaluation of Antioxidant Activity, Inhibitory Activity against α-Glucosidase, Acetylcholinesterase, and Binding Capacity to Human Serum Albumin |
| title_sort | biological activity of extracts of red and yellow fruits of i cornus mas i l an in vitro evaluation of antioxidant activity inhibitory activity against α glucosidase acetylcholinesterase and binding capacity to human serum albumin |
| topic | <i>Cornus mas</i> L. α-glucosidase acetylcholinesterase enzyme inhibition albumin antioxidant activity |
| url | https://www.mdpi.com/1420-3049/27/7/2244 |
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