Extracts from Phyllanthus emblica L stem barks ameliorate blood glucose level and pancreatic and hepatic injuries in streptozotocin-induced diabetic rats
Phyllanthus emblica L is a promising medicinal plant for antidiabetic therapy but current research has not explored its stem bark potential as an antidiabetic agent despite the fact that the stem bark contains rich antioxidants. Herein, we aimed to investigate the antidiabetic potential of P. emblic...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223005440 |
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| author | Quranayati Quranayati Muhammad Iqhrammullah Nurdin Saidi Nurliana Nurliana Rinaldi Idroes Rosnani Nasution |
| author_facet | Quranayati Quranayati Muhammad Iqhrammullah Nurdin Saidi Nurliana Nurliana Rinaldi Idroes Rosnani Nasution |
| author_sort | Quranayati Quranayati |
| collection | DOAJ |
| description | Phyllanthus emblica L is a promising medicinal plant for antidiabetic therapy but current research has not explored its stem bark potential as an antidiabetic agent despite the fact that the stem bark contains rich antioxidants. Herein, we aimed to investigate the antidiabetic potential of P. emblica stem barks in vivo. The dried simplicial powder of the stem barks was macerated sequentially using n-hexane, ethyl acetate, and methanol. The extracts were administered orally to streptozotocin (STZ)-induced diabetic rats, where the blood glucose levels were monitored every week. Histopathological analyses were performed for the pancreas and liver. The results revealed that ethyl acetate extract from P. emblica stem barks could lower the fasting blood glucose levels three weeks post-STZ injection (140.3 ± 38.99 versus 270.0 ± 50.51; p = 0.0375). Significant improvement on pancreas and liver histopathology at p < 0.05 was found in the group receiving P. emblica extracts. The IC50 of the ethyl acetate extract against DPPH free radical was 4.67 ± 1.2 mg/g, with total flavonoids, phenolics, and tannins content of 65.54 ± 0.11 QE mg/g, 35.72 ± 0.5 mg GAE/g, and 134.69 ± 0.7 mg TAE/g, respectively. Taken altogether, the ethyl acetate extract from P. emblica stem barks is potential as an antidiabetic drug candidate. |
| first_indexed | 2024-03-12T22:52:53Z |
| format | Article |
| id | doaj.art-4845a3da353745748759bbc14c46aafe |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-12T22:52:53Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-4845a3da353745748759bbc14c46aafe2023-07-20T04:38:05ZengElsevierArabian Journal of Chemistry1878-53522023-09-01169105082Extracts from Phyllanthus emblica L stem barks ameliorate blood glucose level and pancreatic and hepatic injuries in streptozotocin-induced diabetic ratsQuranayati Quranayati0Muhammad Iqhrammullah1Nurdin Saidi2Nurliana Nurliana3Rinaldi Idroes4Rosnani Nasution5Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh 23111, IndonesiaFaculty of Public Health, Universitas Muhammadiyah Aceh, Banda Aceh 23245, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, IndonesiaLaboratory of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia; Corresponding author.Phyllanthus emblica L is a promising medicinal plant for antidiabetic therapy but current research has not explored its stem bark potential as an antidiabetic agent despite the fact that the stem bark contains rich antioxidants. Herein, we aimed to investigate the antidiabetic potential of P. emblica stem barks in vivo. The dried simplicial powder of the stem barks was macerated sequentially using n-hexane, ethyl acetate, and methanol. The extracts were administered orally to streptozotocin (STZ)-induced diabetic rats, where the blood glucose levels were monitored every week. Histopathological analyses were performed for the pancreas and liver. The results revealed that ethyl acetate extract from P. emblica stem barks could lower the fasting blood glucose levels three weeks post-STZ injection (140.3 ± 38.99 versus 270.0 ± 50.51; p = 0.0375). Significant improvement on pancreas and liver histopathology at p < 0.05 was found in the group receiving P. emblica extracts. The IC50 of the ethyl acetate extract against DPPH free radical was 4.67 ± 1.2 mg/g, with total flavonoids, phenolics, and tannins content of 65.54 ± 0.11 QE mg/g, 35.72 ± 0.5 mg GAE/g, and 134.69 ± 0.7 mg TAE/g, respectively. Taken altogether, the ethyl acetate extract from P. emblica stem barks is potential as an antidiabetic drug candidate.http://www.sciencedirect.com/science/article/pii/S1878535223005440Metabolic syndromeAntioxidantDiabetic hepatopathyEthyl acetateTannins |
| spellingShingle | Quranayati Quranayati Muhammad Iqhrammullah Nurdin Saidi Nurliana Nurliana Rinaldi Idroes Rosnani Nasution Extracts from Phyllanthus emblica L stem barks ameliorate blood glucose level and pancreatic and hepatic injuries in streptozotocin-induced diabetic rats Arabian Journal of Chemistry Metabolic syndrome Antioxidant Diabetic hepatopathy Ethyl acetate Tannins |
| title | Extracts from Phyllanthus emblica L stem barks ameliorate blood glucose level and pancreatic and hepatic injuries in streptozotocin-induced diabetic rats |
| title_full | Extracts from Phyllanthus emblica L stem barks ameliorate blood glucose level and pancreatic and hepatic injuries in streptozotocin-induced diabetic rats |
| title_fullStr | Extracts from Phyllanthus emblica L stem barks ameliorate blood glucose level and pancreatic and hepatic injuries in streptozotocin-induced diabetic rats |
| title_full_unstemmed | Extracts from Phyllanthus emblica L stem barks ameliorate blood glucose level and pancreatic and hepatic injuries in streptozotocin-induced diabetic rats |
| title_short | Extracts from Phyllanthus emblica L stem barks ameliorate blood glucose level and pancreatic and hepatic injuries in streptozotocin-induced diabetic rats |
| title_sort | extracts from phyllanthus emblica l stem barks ameliorate blood glucose level and pancreatic and hepatic injuries in streptozotocin induced diabetic rats |
| topic | Metabolic syndrome Antioxidant Diabetic hepatopathy Ethyl acetate Tannins |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223005440 |
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