Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing
Transcriptional terminators are generally viewed as hard endpoints for transcribing RNA polymerases. Here, the authors reimagine terminators as transcriptional valves with predictable read through. They engineer and characterize 1780 valves and use them for multiplexed gene regulation.
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-01-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-28074-5 |