In Vitro Probiotic Potential of Lactic Acid Bacteria Isolated from Aguamiel and Pulque and Antibacterial Activity Against Pathogens
Probiotics can act as a natural barrier against several pathogens, such <i>Helicobacter pylori</i>, a bacterium linked to stomach cancer. The aim of the present study was to isolate and identify lactic acid bacteria (LAB) from pulque and aguamiel, and evaluate their probiotic potential a...
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MDPI AG
2019-02-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/9/3/601 |
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| author | Alicia Cervantes-Elizarrarás Nelly del Socorro Cruz-Cansino Esther Ramírez-Moreno Vicente Vega-Sánchez Norma Velázquez-Guadarrama Quinatzin Yadira Zafra-Rojas Javier Piloni-Martini |
| author_facet | Alicia Cervantes-Elizarrarás Nelly del Socorro Cruz-Cansino Esther Ramírez-Moreno Vicente Vega-Sánchez Norma Velázquez-Guadarrama Quinatzin Yadira Zafra-Rojas Javier Piloni-Martini |
| author_sort | Alicia Cervantes-Elizarrarás |
| collection | DOAJ |
| description | Probiotics can act as a natural barrier against several pathogens, such <i>Helicobacter pylori</i>, a bacterium linked to stomach cancer. The aim of the present study was to isolate and identify lactic acid bacteria (LAB) from pulque and aguamiel, and evaluate their probiotic potential and antimicrobial effect on <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, and <i>Helicobacter pylori</i>. Ten isolates were selected and evaluated for in vitro resistance to antibiotics and gastrointestinal conditions, and antimicrobial activity against <i>E. coli</i> and <i>S. aureus</i> and the effect on <i>H. pylori</i> strains. 16S rRNA identification was performed. Ten potential probiotic isolates were confirmed as belonging to the genera <i>Lactobacillus</i> and <i>Pediococcus</i>. All the strains were susceptible to clinical antibiotics, except to vancomycin. Sixty percent of the isolates exhibited antimicrobial activity against <i>E. coli</i> and <i>S. aureus</i>. The growth of <i>H. pylori</i> ATCC 43504 was suppressed by all the LAB, and the urease activity from all the <i>H. pylori</i> strains was inhibited, which may decrease its chances for survival in the stomach. The results suggest that LAB isolated from pulque and aguamiel could be an option to establish a harmless relationship between the host and <i>H. pylori</i>, helping in their eradication therapy. |
| first_indexed | 2024-12-10T17:11:25Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-10T17:11:25Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-4848b8ef4c0540e08aabb9d97bf1f8d72022-12-22T01:40:18ZengMDPI AGApplied Sciences2076-34172019-02-019360110.3390/app9030601app9030601In Vitro Probiotic Potential of Lactic Acid Bacteria Isolated from Aguamiel and Pulque and Antibacterial Activity Against PathogensAlicia Cervantes-Elizarrarás0Nelly del Socorro Cruz-Cansino1Esther Ramírez-Moreno2Vicente Vega-Sánchez3Norma Velázquez-Guadarrama4Quinatzin Yadira Zafra-Rojas5Javier Piloni-Martini6Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Circuito Ex Hacienda La Concepción S/N, Carretera Pachuca-Actopan, San Agustín Tlaxiaca 42160, Hidalgo, MexicoInstituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Circuito Ex Hacienda La Concepción S/N, Carretera Pachuca-Actopan, San Agustín Tlaxiaca 42160, Hidalgo, MexicoInstituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Circuito Ex Hacienda La Concepción S/N, Carretera Pachuca-Actopan, San Agustín Tlaxiaca 42160, Hidalgo, MexicoInstituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo. Av. Universidad Km 1, Rancho Universitario, Tulancingo 43600, Hidalgo, MexicoLaboratorio de Infectología, Departamento de Infectología, Hospital Infantil de México Federico Gómez, Col. Doctores, Delegación: Cuauhtémoc, Ciudad de México 06720, MexicoInstituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Circuito Ex Hacienda La Concepción S/N, Carretera Pachuca-Actopan, San Agustín Tlaxiaca 42160, Hidalgo, MexicoInstituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo. Av. Universidad Km 1, Rancho Universitario, Tulancingo 43600, Hidalgo, MexicoProbiotics can act as a natural barrier against several pathogens, such <i>Helicobacter pylori</i>, a bacterium linked to stomach cancer. The aim of the present study was to isolate and identify lactic acid bacteria (LAB) from pulque and aguamiel, and evaluate their probiotic potential and antimicrobial effect on <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, and <i>Helicobacter pylori</i>. Ten isolates were selected and evaluated for in vitro resistance to antibiotics and gastrointestinal conditions, and antimicrobial activity against <i>E. coli</i> and <i>S. aureus</i> and the effect on <i>H. pylori</i> strains. 16S rRNA identification was performed. Ten potential probiotic isolates were confirmed as belonging to the genera <i>Lactobacillus</i> and <i>Pediococcus</i>. All the strains were susceptible to clinical antibiotics, except to vancomycin. Sixty percent of the isolates exhibited antimicrobial activity against <i>E. coli</i> and <i>S. aureus</i>. The growth of <i>H. pylori</i> ATCC 43504 was suppressed by all the LAB, and the urease activity from all the <i>H. pylori</i> strains was inhibited, which may decrease its chances for survival in the stomach. The results suggest that LAB isolated from pulque and aguamiel could be an option to establish a harmless relationship between the host and <i>H. pylori</i>, helping in their eradication therapy.https://www.mdpi.com/2076-3417/9/3/601antimicrobial activitygastrointestinal conditions<i>Helicobacter pylori</i><i>Lactobacillus</i><i>Pediococcus</i>urease |
| spellingShingle | Alicia Cervantes-Elizarrarás Nelly del Socorro Cruz-Cansino Esther Ramírez-Moreno Vicente Vega-Sánchez Norma Velázquez-Guadarrama Quinatzin Yadira Zafra-Rojas Javier Piloni-Martini In Vitro Probiotic Potential of Lactic Acid Bacteria Isolated from Aguamiel and Pulque and Antibacterial Activity Against Pathogens Applied Sciences antimicrobial activity gastrointestinal conditions <i>Helicobacter pylori</i> <i>Lactobacillus</i> <i>Pediococcus</i> urease |
| title | In Vitro Probiotic Potential of Lactic Acid Bacteria Isolated from Aguamiel and Pulque and Antibacterial Activity Against Pathogens |
| title_full | In Vitro Probiotic Potential of Lactic Acid Bacteria Isolated from Aguamiel and Pulque and Antibacterial Activity Against Pathogens |
| title_fullStr | In Vitro Probiotic Potential of Lactic Acid Bacteria Isolated from Aguamiel and Pulque and Antibacterial Activity Against Pathogens |
| title_full_unstemmed | In Vitro Probiotic Potential of Lactic Acid Bacteria Isolated from Aguamiel and Pulque and Antibacterial Activity Against Pathogens |
| title_short | In Vitro Probiotic Potential of Lactic Acid Bacteria Isolated from Aguamiel and Pulque and Antibacterial Activity Against Pathogens |
| title_sort | in vitro probiotic potential of lactic acid bacteria isolated from aguamiel and pulque and antibacterial activity against pathogens |
| topic | antimicrobial activity gastrointestinal conditions <i>Helicobacter pylori</i> <i>Lactobacillus</i> <i>Pediococcus</i> urease |
| url | https://www.mdpi.com/2076-3417/9/3/601 |
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