Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and Conformation
The pectic polysaccharides extracted from flaxseed (<i>Linum usitatissiumum</i> L.) mucilage and kernel were characterized as rhamnogalacturonan-I (RG-I). In this study, the conformational characteristics of RG-I fractions from flaxseed mucilage and kernel were investigated, using a Broo...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/14/13/2667 |
| _version_ | 1797408298247389184 |
|---|---|
| author | Qingbin Guo Zhengxin Shan Yanhui Shao Nifei Wang Keying Qian H. Douglas Goff Qi Wang Steve W. Cui Huihuang H. Ding |
| author_facet | Qingbin Guo Zhengxin Shan Yanhui Shao Nifei Wang Keying Qian H. Douglas Goff Qi Wang Steve W. Cui Huihuang H. Ding |
| author_sort | Qingbin Guo |
| collection | DOAJ |
| description | The pectic polysaccharides extracted from flaxseed (<i>Linum usitatissiumum</i> L.) mucilage and kernel were characterized as rhamnogalacturonan-I (RG-I). In this study, the conformational characteristics of RG-I fractions from flaxseed mucilage and kernel were investigated, using a Brookhaven multi-angle light scattering instrument (batch mode) and a high-performance size exclusion chromatography (HPSEC) system coupled with Viscotek tetra-detectors (flow mode). The <i>M<sub>w</sub></i> of flaxseed mucilage RG-I (FM-R) was 285 kDa, and the structure-sensitive parameter (<i>ρ</i>) value of FM-R was calculated as 1.3, suggesting that the FM-R molecule had a star-like conformation. The <i>M<sub>w</sub></i> of flaxseed kernel RG-I (FK-R) was 550 kDa, and the structure-sensitive parameter (<i>ρ</i>) values ranged from 0.90 to 1.21, suggesting a sphere to star-like conformation with relatively higher segment density. The correlation between the primary structure and conformation of RG-I was further discussed to better understand the structure–function relationship, which helps the scale-up applications of pectins in food, pharmaceutical, or cosmetic industries. |
| first_indexed | 2024-03-09T03:57:23Z |
| format | Article |
| id | doaj.art-484ad22387cd4e1c9ebdd6730ff0c7ba |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T03:57:23Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-484ad22387cd4e1c9ebdd6730ff0c7ba2023-12-03T14:18:56ZengMDPI AGPolymers2073-43602022-06-011413266710.3390/polym14132667Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and ConformationQingbin Guo0Zhengxin Shan1Yanhui Shao2Nifei Wang3Keying Qian4H. Douglas Goff5Qi Wang6Steve W. Cui7Huihuang H. Ding8State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaDepartment of Food Science, University of Guelph, 50 Stone Road E., Guelph, ON N1G 2W1, CanadaDepartment of Food Science, University of Guelph, 50 Stone Road E., Guelph, ON N1G 2W1, CanadaGuelph Research and Development Centre, Agriculture and Agri-Food Canada, 93 Stone Road W., Guelph, ON N1G 5C9, CanadaDepartment of Food Science, University of Guelph, 50 Stone Road E., Guelph, ON N1G 2W1, CanadaDepartment of Food Science, University of Guelph, 50 Stone Road E., Guelph, ON N1G 2W1, CanadaThe pectic polysaccharides extracted from flaxseed (<i>Linum usitatissiumum</i> L.) mucilage and kernel were characterized as rhamnogalacturonan-I (RG-I). In this study, the conformational characteristics of RG-I fractions from flaxseed mucilage and kernel were investigated, using a Brookhaven multi-angle light scattering instrument (batch mode) and a high-performance size exclusion chromatography (HPSEC) system coupled with Viscotek tetra-detectors (flow mode). The <i>M<sub>w</sub></i> of flaxseed mucilage RG-I (FM-R) was 285 kDa, and the structure-sensitive parameter (<i>ρ</i>) value of FM-R was calculated as 1.3, suggesting that the FM-R molecule had a star-like conformation. The <i>M<sub>w</sub></i> of flaxseed kernel RG-I (FK-R) was 550 kDa, and the structure-sensitive parameter (<i>ρ</i>) values ranged from 0.90 to 1.21, suggesting a sphere to star-like conformation with relatively higher segment density. The correlation between the primary structure and conformation of RG-I was further discussed to better understand the structure–function relationship, which helps the scale-up applications of pectins in food, pharmaceutical, or cosmetic industries.https://www.mdpi.com/2073-4360/14/13/2667dietary fibreflaxseedpectinpolysaccharideRG-Istructure–function relationship |
| spellingShingle | Qingbin Guo Zhengxin Shan Yanhui Shao Nifei Wang Keying Qian H. Douglas Goff Qi Wang Steve W. Cui Huihuang H. Ding Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and Conformation Polymers dietary fibre flaxseed pectin polysaccharide RG-I structure–function relationship |
| title | Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and Conformation |
| title_full | Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and Conformation |
| title_fullStr | Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and Conformation |
| title_full_unstemmed | Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and Conformation |
| title_short | Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and Conformation |
| title_sort | conformational properties of flaxseed rhamnogalacturonan i and correlation between primary structure and conformation |
| topic | dietary fibre flaxseed pectin polysaccharide RG-I structure–function relationship |
| url | https://www.mdpi.com/2073-4360/14/13/2667 |
| work_keys_str_mv | AT qingbinguo conformationalpropertiesofflaxseedrhamnogalacturonaniandcorrelationbetweenprimarystructureandconformation AT zhengxinshan conformationalpropertiesofflaxseedrhamnogalacturonaniandcorrelationbetweenprimarystructureandconformation AT yanhuishao conformationalpropertiesofflaxseedrhamnogalacturonaniandcorrelationbetweenprimarystructureandconformation AT nifeiwang conformationalpropertiesofflaxseedrhamnogalacturonaniandcorrelationbetweenprimarystructureandconformation AT keyingqian conformationalpropertiesofflaxseedrhamnogalacturonaniandcorrelationbetweenprimarystructureandconformation AT hdouglasgoff conformationalpropertiesofflaxseedrhamnogalacturonaniandcorrelationbetweenprimarystructureandconformation AT qiwang conformationalpropertiesofflaxseedrhamnogalacturonaniandcorrelationbetweenprimarystructureandconformation AT stevewcui conformationalpropertiesofflaxseedrhamnogalacturonaniandcorrelationbetweenprimarystructureandconformation AT huihuanghding conformationalpropertiesofflaxseedrhamnogalacturonaniandcorrelationbetweenprimarystructureandconformation |