Enhancement of cucumber resistance under salt stress by 2, 4-epibrassinolide lactones
This study investigated the effects of exogenous 2, 4-epibrassinolide lactone (EBR) on the growth, photosynthetic pigments, antioxidant defense system, ion homeostasis, MAPK cascade and key genes of SOS signaling pathway of cucumber seedlings under salt stress using cucumber “Xinchun 4” as the test...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1023178/full |
| _version_ | 1811231336732557312 |
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| author | Xianxia He Zilong Wan Ning Jin Li Jin Guobin Zhang Jian Lyu Zeci Liu Shilei Luo Jihua Yu Jihua Yu |
| author_facet | Xianxia He Zilong Wan Ning Jin Li Jin Guobin Zhang Jian Lyu Zeci Liu Shilei Luo Jihua Yu Jihua Yu |
| author_sort | Xianxia He |
| collection | DOAJ |
| description | This study investigated the effects of exogenous 2, 4-epibrassinolide lactone (EBR) on the growth, photosynthetic pigments, antioxidant defense system, ion homeostasis, MAPK cascade and key genes of SOS signaling pathway of cucumber seedlings under salt stress using cucumber “Xinchun 4” as the test material. The experiment was set up with four treatments: foliar spraying of distilled water (CK), 50 mmol.L-1 NaCl (NaCl), 50 mmol.L-1 NaCl+foliar spray of 0.02 μmol.L-1 EBR (EBR+NaCl), and 50 mmol.L-1 NaCl+foliar spray of 24 μmol.L-1 Brassinazole (BRZ) (BRZ+NaCl). The results showed that EBR+NaCl treatment significantly increased plant height, above-ground fresh weight, total root length, total root surface area, average rhizome and photosynthetic pigment content compared to NaCl treatment. Meanwhile, compared with NaCl treatment, EBR+NaCl treatment significantly increased superoxide dismutase, catalase and ascorbate peroxidase (SOD, CAT and APX) activities, significantly promoted the accumulation of osmoregulatory substances (soluble sugars and proline), and thus effectively reduced malondialdehyde (MDA) content and relative electrical conductivity of cucumber leaves. Exogenous spraying of EBR also significantly reduced Na+/K+ under NaCl stress, effectively alleviating the toxic effects of Na+ ions. In addition, exogenous EBR induced the up-regulated expression of CsMAPK3, CsMAPK4, CsMAPK6 and CsMAPK9 genes in the MAPK cascade signaling pathway and CsSOS1, CsSOS2 and CsSOS3 genes in the SOS signaling pathway to enhance salt tolerance in cucumber under NaCl stress. Therefore, exogenous spraying EBR may effectively reduce the damage of salt stress on cucumber seedlings by improving antioxidant capacity, maintaining ion homeostasis and activating salt-tolerant related signaling pathways, which might promote the growth of cucumber seedlings and the establishment of root system morphology. This study provides a reference for EBR to improve the salt tolerance of cucumber. |
| first_indexed | 2024-04-12T10:43:25Z |
| format | Article |
| id | doaj.art-484bd5b60b5849268cbe26a16c7b0199 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T10:43:25Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-484bd5b60b5849268cbe26a16c7b01992022-12-22T03:36:31ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10231781023178Enhancement of cucumber resistance under salt stress by 2, 4-epibrassinolide lactonesXianxia He0Zilong Wan1Ning Jin2Li Jin3Guobin Zhang4Jian Lyu5Zeci Liu6Shilei Luo7Jihua Yu8Jihua Yu9College of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Arid land Crop Science, Gansu Agricultural University, Lanzhou, ChinaThis study investigated the effects of exogenous 2, 4-epibrassinolide lactone (EBR) on the growth, photosynthetic pigments, antioxidant defense system, ion homeostasis, MAPK cascade and key genes of SOS signaling pathway of cucumber seedlings under salt stress using cucumber “Xinchun 4” as the test material. The experiment was set up with four treatments: foliar spraying of distilled water (CK), 50 mmol.L-1 NaCl (NaCl), 50 mmol.L-1 NaCl+foliar spray of 0.02 μmol.L-1 EBR (EBR+NaCl), and 50 mmol.L-1 NaCl+foliar spray of 24 μmol.L-1 Brassinazole (BRZ) (BRZ+NaCl). The results showed that EBR+NaCl treatment significantly increased plant height, above-ground fresh weight, total root length, total root surface area, average rhizome and photosynthetic pigment content compared to NaCl treatment. Meanwhile, compared with NaCl treatment, EBR+NaCl treatment significantly increased superoxide dismutase, catalase and ascorbate peroxidase (SOD, CAT and APX) activities, significantly promoted the accumulation of osmoregulatory substances (soluble sugars and proline), and thus effectively reduced malondialdehyde (MDA) content and relative electrical conductivity of cucumber leaves. Exogenous spraying of EBR also significantly reduced Na+/K+ under NaCl stress, effectively alleviating the toxic effects of Na+ ions. In addition, exogenous EBR induced the up-regulated expression of CsMAPK3, CsMAPK4, CsMAPK6 and CsMAPK9 genes in the MAPK cascade signaling pathway and CsSOS1, CsSOS2 and CsSOS3 genes in the SOS signaling pathway to enhance salt tolerance in cucumber under NaCl stress. Therefore, exogenous spraying EBR may effectively reduce the damage of salt stress on cucumber seedlings by improving antioxidant capacity, maintaining ion homeostasis and activating salt-tolerant related signaling pathways, which might promote the growth of cucumber seedlings and the establishment of root system morphology. This study provides a reference for EBR to improve the salt tolerance of cucumber.https://www.frontiersin.org/articles/10.3389/fpls.2022.1023178/fullcucumbersalt stress2, 4-epibrassinolideantioxidantMAPKSOS |
| spellingShingle | Xianxia He Zilong Wan Ning Jin Li Jin Guobin Zhang Jian Lyu Zeci Liu Shilei Luo Jihua Yu Jihua Yu Enhancement of cucumber resistance under salt stress by 2, 4-epibrassinolide lactones Frontiers in Plant Science cucumber salt stress 2, 4-epibrassinolide antioxidant MAPK SOS |
| title | Enhancement of cucumber resistance under salt stress by 2, 4-epibrassinolide lactones |
| title_full | Enhancement of cucumber resistance under salt stress by 2, 4-epibrassinolide lactones |
| title_fullStr | Enhancement of cucumber resistance under salt stress by 2, 4-epibrassinolide lactones |
| title_full_unstemmed | Enhancement of cucumber resistance under salt stress by 2, 4-epibrassinolide lactones |
| title_short | Enhancement of cucumber resistance under salt stress by 2, 4-epibrassinolide lactones |
| title_sort | enhancement of cucumber resistance under salt stress by 2 4 epibrassinolide lactones |
| topic | cucumber salt stress 2, 4-epibrassinolide antioxidant MAPK SOS |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1023178/full |
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