Allergic reactions to tick saliva components in zebrafish model
Abstract Background Alpha-Gal syndrome (AGS) is a tick-borne food allergy caused by IgE antibodies against the glycan galactose-alpha-1,3-galactose (α-Gal) present in glycoproteins and glycolipids from mammalian meat. To advance in the diagnosis and treatment of AGS, further research is needed to un...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-023-05874-2 |
| _version_ | 1797453733428199424 |
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| author | Marinela Contreras Rita Vaz-Rodrigues Lorena Mazuecos Margarita Villar Sara Artigas-Jerónimo Almudena González-García Nadezhda V. Shilova Nicolai V. Bovin Sandra Díaz-Sánchez Elisa Ferreras-Colino Iván Pacheco Jindřich Chmelař Petr Kopáček Alejandro Cabezas-Cruz Christian Gortázar José de la Fuente |
| author_facet | Marinela Contreras Rita Vaz-Rodrigues Lorena Mazuecos Margarita Villar Sara Artigas-Jerónimo Almudena González-García Nadezhda V. Shilova Nicolai V. Bovin Sandra Díaz-Sánchez Elisa Ferreras-Colino Iván Pacheco Jindřich Chmelař Petr Kopáček Alejandro Cabezas-Cruz Christian Gortázar José de la Fuente |
| author_sort | Marinela Contreras |
| collection | DOAJ |
| description | Abstract Background Alpha-Gal syndrome (AGS) is a tick-borne food allergy caused by IgE antibodies against the glycan galactose-alpha-1,3-galactose (α-Gal) present in glycoproteins and glycolipids from mammalian meat. To advance in the diagnosis and treatment of AGS, further research is needed to unravel the molecular and immune mechanisms underlying this syndrome. The objective of this study is the characterization of tick salivary components and proteins with and without α-Gal modifications involved in modulating human immune response against this carbohydrate. Methods Protein and α-Gal content were determined in tick saliva components, and proteins were identified by proteomics analysis of tick saliva fractions. Pathophysiological changes were recorded in the zebrafish (Danio rerio) model after exposure to distinct Ixodes ricinus tick salivary components. Serum samples were collected from zebrafish at day 8 of exposure to determine anti-α-Gal, anti-glycan, and anti-tick saliva protein IgM antibody titers by enzyme-linked immunosorbent assay (ELISA). Results Zebrafish treated with tick saliva and saliva protein fractions combined with non-protein fractions demonstrated significantly higher incidence of hemorrhagic type allergic reactions, abnormal behavioral patterns, or mortality when compared to the phosphate-buffered saline (PBS)-treated control group. The main tick salivary proteins identified in these fractions with possible functional implication in AGS were the secreted protein B7P208-salivary antigen p23 and metalloproteases. Anti-α-Gal and anti-tick salivary gland IgM antibody titers were significantly higher in distinct saliva protein fractions and deglycosylated saliva group when compared with PBS-treated controls. Anti-glycan antibodies showed group-related profiles. Conclusions Results support the hypothesis that tick salivary biomolecules with and without α-Gal modifications are involved in modulating immune response against this carbohydrate. Graphical Abstract |
| first_indexed | 2024-03-09T15:27:06Z |
| format | Article |
| id | doaj.art-484daa4ce84642cc9dbee59341308fd5 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-09T15:27:06Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-484daa4ce84642cc9dbee59341308fd52023-11-26T12:29:00ZengBMCParasites & Vectors1756-33052023-07-0116111510.1186/s13071-023-05874-2Allergic reactions to tick saliva components in zebrafish modelMarinela Contreras0Rita Vaz-Rodrigues1Lorena Mazuecos2Margarita Villar3Sara Artigas-Jerónimo4Almudena González-García5Nadezhda V. Shilova6Nicolai V. Bovin7Sandra Díaz-Sánchez8Elisa Ferreras-Colino9Iván Pacheco10Jindřich Chmelař11Petr Kopáček12Alejandro Cabezas-Cruz13Christian Gortázar14José de la Fuente15SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCMSaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCMSaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCMSaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCMSaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCMSaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCMShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesSaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCMSaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCMSaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCMDepartment of Medical Biology, Faculty of Science, University of South Bohemia in České BudějoviceInstitute of ParasitologyBiology Centre, Czech Academy of SciencesUMR BIPAR, INRAE, ANSES, Ecole Nationale Vétérinaire d’Alfort, Université Paris-EstSaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCMSaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCMAbstract Background Alpha-Gal syndrome (AGS) is a tick-borne food allergy caused by IgE antibodies against the glycan galactose-alpha-1,3-galactose (α-Gal) present in glycoproteins and glycolipids from mammalian meat. To advance in the diagnosis and treatment of AGS, further research is needed to unravel the molecular and immune mechanisms underlying this syndrome. The objective of this study is the characterization of tick salivary components and proteins with and without α-Gal modifications involved in modulating human immune response against this carbohydrate. Methods Protein and α-Gal content were determined in tick saliva components, and proteins were identified by proteomics analysis of tick saliva fractions. Pathophysiological changes were recorded in the zebrafish (Danio rerio) model after exposure to distinct Ixodes ricinus tick salivary components. Serum samples were collected from zebrafish at day 8 of exposure to determine anti-α-Gal, anti-glycan, and anti-tick saliva protein IgM antibody titers by enzyme-linked immunosorbent assay (ELISA). Results Zebrafish treated with tick saliva and saliva protein fractions combined with non-protein fractions demonstrated significantly higher incidence of hemorrhagic type allergic reactions, abnormal behavioral patterns, or mortality when compared to the phosphate-buffered saline (PBS)-treated control group. The main tick salivary proteins identified in these fractions with possible functional implication in AGS were the secreted protein B7P208-salivary antigen p23 and metalloproteases. Anti-α-Gal and anti-tick salivary gland IgM antibody titers were significantly higher in distinct saliva protein fractions and deglycosylated saliva group when compared with PBS-treated controls. Anti-glycan antibodies showed group-related profiles. Conclusions Results support the hypothesis that tick salivary biomolecules with and without α-Gal modifications are involved in modulating immune response against this carbohydrate. Graphical Abstracthttps://doi.org/10.1186/s13071-023-05874-2AllergyAlpha-gal syndromeGlycanTickZebrafish |
| spellingShingle | Marinela Contreras Rita Vaz-Rodrigues Lorena Mazuecos Margarita Villar Sara Artigas-Jerónimo Almudena González-García Nadezhda V. Shilova Nicolai V. Bovin Sandra Díaz-Sánchez Elisa Ferreras-Colino Iván Pacheco Jindřich Chmelař Petr Kopáček Alejandro Cabezas-Cruz Christian Gortázar José de la Fuente Allergic reactions to tick saliva components in zebrafish model Parasites & Vectors Allergy Alpha-gal syndrome Glycan Tick Zebrafish |
| title | Allergic reactions to tick saliva components in zebrafish model |
| title_full | Allergic reactions to tick saliva components in zebrafish model |
| title_fullStr | Allergic reactions to tick saliva components in zebrafish model |
| title_full_unstemmed | Allergic reactions to tick saliva components in zebrafish model |
| title_short | Allergic reactions to tick saliva components in zebrafish model |
| title_sort | allergic reactions to tick saliva components in zebrafish model |
| topic | Allergy Alpha-gal syndrome Glycan Tick Zebrafish |
| url | https://doi.org/10.1186/s13071-023-05874-2 |
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