First detection of vanA positive Enterococcus faecium clonal complex 17 in hospital wastewater in Algeria: an epidemiological report
Enterococcus spp. are Gram-positive cocci that are recognised as critical opportunistic pathogens, especially in immunocompromised patients. Vancomycin is considered as the drug of last resort for the treatment of infections caused by Enterococcus species, making vancomycin resistance a serious publ...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-04-01
|
| Series: | New Microbes and New Infections |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2052297522000294 |
| _version_ | 1818158498329919488 |
|---|---|
| author | Z. Cherak E. Bendjama A. Moussi A. Benbouza N. Grainat J.-M. Rolain L. Loucif |
| author_facet | Z. Cherak E. Bendjama A. Moussi A. Benbouza N. Grainat J.-M. Rolain L. Loucif |
| author_sort | Z. Cherak |
| collection | DOAJ |
| description | Enterococcus spp. are Gram-positive cocci that are recognised as critical opportunistic pathogens, especially in immunocompromised patients. Vancomycin is considered as the drug of last resort for the treatment of infections caused by Enterococcus species, making vancomycin resistance a serious public health concern. In this article, we report the first environmental vanA positive Enterococcus faecium isolates in Algeria. The strains were selectively isolated from hospital wastewater and then identified using matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry. Antibiotic susceptibility testing was performed using the disc diffusion method. Vancomycin resistance genes were searched for by standard PCR and the clonal relatedness of our isolates was investigated by multilocus sequence typing. A total of five highly vancomycin-resistant Gram-positive bacteria were isolated and identified as Enterococcus faecium. The isolates harboured the vanA gene and were assigned to the clonal complex 17. Our findings confirm the great potential of hospital wastewater as a reservoir and dissemination pathway of multidrug resistant organisms, and alert to the need for better regulation of hospital waste management in order to reduce their impact on the environment. |
| first_indexed | 2024-12-11T15:31:02Z |
| format | Article |
| id | doaj.art-485143a980eb42a2aac10480d66ab634 |
| institution | Directory Open Access Journal |
| issn | 2052-2975 |
| language | English |
| last_indexed | 2024-12-11T15:31:02Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | New Microbes and New Infections |
| spelling | doaj.art-485143a980eb42a2aac10480d66ab6342022-12-22T01:00:03ZengElsevierNew Microbes and New Infections2052-29752022-04-0147100977First detection of vanA positive Enterococcus faecium clonal complex 17 in hospital wastewater in Algeria: an epidemiological reportZ. Cherak0E. Bendjama1A. Moussi2A. Benbouza3N. Grainat4J.-M. Rolain5L. Loucif6Laboratoire de Génétique, Biotechnologie et Valorisation des Bio-ressources (GBVB), Faculté des Sciences Exactes et des Sciences de La Nature et de La Vie, Université Mohamed Khider, Biskra, Algeria; Faculté des Sciences de La Nature et de La Vie, Université de Batna 2, Batna, AlgeriaLaboratoire de Biotechnologie des Molécules Bioactives et de La Physiopathologie Cellulaire (LBMBPC), Faculté des Sciences de La Nature et de La Vie, Université de Batna 2, Batna, Algeria; Département de Technologie Alimentaire, Institut des Sciences Vétérinaires et des Sciences Agronomiques, Université El Hadj Lakhder-Batna 1, Batna, AlgeriaLaboratoire de Génétique, Biotechnologie et Valorisation des Bio-ressources (GBVB), Faculté des Sciences Exactes et des Sciences de La Nature et de La Vie, Université Mohamed Khider, Biskra, AlgeriaFaculté de Médecine, Université de Batna 2, Batna, AlgeriaFaculté de Médecine, Université de Batna 2, Batna, AlgeriaAix Marseille Univ, IRD, MEPHI, Faculté de Médecine et de Pharmacie, Marseille, France; IHU Méditerranée Infection, Marseille, France; Assistance Publique des Hôpitaux de Marseille, Marseille, FranceLaboratoire de Biotechnologie des Molécules Bioactives et de La Physiopathologie Cellulaire (LBMBPC), Faculté des Sciences de La Nature et de La Vie, Université de Batna 2, Batna, Algeria; Corresponding author: L. LoucifEnterococcus spp. are Gram-positive cocci that are recognised as critical opportunistic pathogens, especially in immunocompromised patients. Vancomycin is considered as the drug of last resort for the treatment of infections caused by Enterococcus species, making vancomycin resistance a serious public health concern. In this article, we report the first environmental vanA positive Enterococcus faecium isolates in Algeria. The strains were selectively isolated from hospital wastewater and then identified using matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry. Antibiotic susceptibility testing was performed using the disc diffusion method. Vancomycin resistance genes were searched for by standard PCR and the clonal relatedness of our isolates was investigated by multilocus sequence typing. A total of five highly vancomycin-resistant Gram-positive bacteria were isolated and identified as Enterococcus faecium. The isolates harboured the vanA gene and were assigned to the clonal complex 17. Our findings confirm the great potential of hospital wastewater as a reservoir and dissemination pathway of multidrug resistant organisms, and alert to the need for better regulation of hospital waste management in order to reduce their impact on the environment.http://www.sciencedirect.com/science/article/pii/S2052297522000294AlgeriaEnterococcus faeciumhospital wastewatervanAvancomycin resistance |
| spellingShingle | Z. Cherak E. Bendjama A. Moussi A. Benbouza N. Grainat J.-M. Rolain L. Loucif First detection of vanA positive Enterococcus faecium clonal complex 17 in hospital wastewater in Algeria: an epidemiological report New Microbes and New Infections Algeria Enterococcus faecium hospital wastewater vanA vancomycin resistance |
| title | First detection of vanA positive Enterococcus faecium clonal complex 17 in hospital wastewater in Algeria: an epidemiological report |
| title_full | First detection of vanA positive Enterococcus faecium clonal complex 17 in hospital wastewater in Algeria: an epidemiological report |
| title_fullStr | First detection of vanA positive Enterococcus faecium clonal complex 17 in hospital wastewater in Algeria: an epidemiological report |
| title_full_unstemmed | First detection of vanA positive Enterococcus faecium clonal complex 17 in hospital wastewater in Algeria: an epidemiological report |
| title_short | First detection of vanA positive Enterococcus faecium clonal complex 17 in hospital wastewater in Algeria: an epidemiological report |
| title_sort | first detection of vana positive enterococcus faecium clonal complex 17 in hospital wastewater in algeria an epidemiological report |
| topic | Algeria Enterococcus faecium hospital wastewater vanA vancomycin resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2052297522000294 |
| work_keys_str_mv | AT zcherak firstdetectionofvanapositiveenterococcusfaeciumclonalcomplex17inhospitalwastewaterinalgeriaanepidemiologicalreport AT ebendjama firstdetectionofvanapositiveenterococcusfaeciumclonalcomplex17inhospitalwastewaterinalgeriaanepidemiologicalreport AT amoussi firstdetectionofvanapositiveenterococcusfaeciumclonalcomplex17inhospitalwastewaterinalgeriaanepidemiologicalreport AT abenbouza firstdetectionofvanapositiveenterococcusfaeciumclonalcomplex17inhospitalwastewaterinalgeriaanepidemiologicalreport AT ngrainat firstdetectionofvanapositiveenterococcusfaeciumclonalcomplex17inhospitalwastewaterinalgeriaanepidemiologicalreport AT jmrolain firstdetectionofvanapositiveenterococcusfaeciumclonalcomplex17inhospitalwastewaterinalgeriaanepidemiologicalreport AT lloucif firstdetectionofvanapositiveenterococcusfaeciumclonalcomplex17inhospitalwastewaterinalgeriaanepidemiologicalreport |