The different variant allele frequencies of type I/type II mutations and the distinct molecular landscapes in CALR-mutant essential thrombocythaemia and primary myelofibrosis
Objective Calreticulin (CALR) mutations have been identified as driver mutations in a quarter of patients with essential thrombocythaemia (ET) and primary myelofibrosis (PMF), which are subgroups of myeloproliferative neoplasms (MPNs). A 52-bp deletion (type I mutation) and a 5-bp insertion (type II...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2022-12-01
|
Series: | Hematology |
Subjects: | |
Online Access: | https://www.tandfonline.com/doi/10.1080/16078454.2022.2107888 |
_version_ | 1798036642315894784 |
---|---|
author | Yuxia Pan Xingzhe Wang Shupeng Wen Xiaojun Liu Lin Yang Jianmin Luo |
author_facet | Yuxia Pan Xingzhe Wang Shupeng Wen Xiaojun Liu Lin Yang Jianmin Luo |
author_sort | Yuxia Pan |
collection | DOAJ |
description | Objective Calreticulin (CALR) mutations have been identified as driver mutations in a quarter of patients with essential thrombocythaemia (ET) and primary myelofibrosis (PMF), which are subgroups of myeloproliferative neoplasms (MPNs). A 52-bp deletion (type I mutation) and a 5-bp insertion (type II mutation) are the most frequent variants. To better understand the impact of different CALR mutant variants, with or without nondriver mutations, on the clinical subtypes of MPN needs further investigation.Methods The clinical characteristics, laboratory parameters and genetic mutation statuses were analysed in a cohort of 77 MPN patients with CALR mutations (ET = 24, prePMF = 33, and overt PMF = 20). Targeted NGS using a 38-gene panel was performed to evaluate the variant allele frequency (VAF) of CALR type I/type II mutations and assess the molecular landscape of nondriver gene mutations.Results A lower VAF of type I vs. type II was observed in CALR-mutant ET, prePMF and overt PMF, and a higher frequency of type I vs. type II was found in CALR-mutant overt PMF. Additional somatic mutations were indicated to be useful in understanding the pathogenesis of MPN. In this study, the mutation landscape was more complex in overt PMF than in ET or in prePMF. Mutations in epigenetic regulators (ASXL1, EZH2 and TET2) were more common in overt PMF.Conclusions The two different subtypes of CALR mutations may have different impacts on MPN. A lower VAF of CALR type I indicates a greater contribution to disease progression in MPN, and increased nondriver mutations may be important in myelofibrosis progression. |
first_indexed | 2024-04-11T21:15:42Z |
format | Article |
id | doaj.art-4853e2cb2c38476abcfb36a24611a98e |
institution | Directory Open Access Journal |
issn | 1607-8454 |
language | English |
last_indexed | 2024-04-11T21:15:42Z |
publishDate | 2022-12-01 |
publisher | Taylor & Francis Group |
record_format | Article |
series | Hematology |
spelling | doaj.art-4853e2cb2c38476abcfb36a24611a98e2022-12-22T04:02:49ZengTaylor & Francis GroupHematology1607-84542022-12-0127190290810.1080/16078454.2022.2107888The different variant allele frequencies of type I/type II mutations and the distinct molecular landscapes in CALR-mutant essential thrombocythaemia and primary myelofibrosisYuxia Pan0Xingzhe Wang1Shupeng Wen2Xiaojun Liu3Lin Yang4Jianmin Luo5Department of Hematology, The Second Hospital of Hebei Medical University, Key Laboratory of Hematology, Shijiazhuang, People’s Republic of ChinaDepartment of Hematology, The Second Hospital of Hebei Medical University, Key Laboratory of Hematology, Shijiazhuang, People’s Republic of ChinaDepartment of Hematology, The Second Hospital of Hebei Medical University, Key Laboratory of Hematology, Shijiazhuang, People’s Republic of ChinaDepartment of Hematology, The Second Hospital of Hebei Medical University, Key Laboratory of Hematology, Shijiazhuang, People’s Republic of ChinaDepartment of Hematology, The Second Hospital of Hebei Medical University, Key Laboratory of Hematology, Shijiazhuang, People’s Republic of ChinaDepartment of Hematology, The Second Hospital of Hebei Medical University, Key Laboratory of Hematology, Shijiazhuang, People’s Republic of ChinaObjective Calreticulin (CALR) mutations have been identified as driver mutations in a quarter of patients with essential thrombocythaemia (ET) and primary myelofibrosis (PMF), which are subgroups of myeloproliferative neoplasms (MPNs). A 52-bp deletion (type I mutation) and a 5-bp insertion (type II mutation) are the most frequent variants. To better understand the impact of different CALR mutant variants, with or without nondriver mutations, on the clinical subtypes of MPN needs further investigation.Methods The clinical characteristics, laboratory parameters and genetic mutation statuses were analysed in a cohort of 77 MPN patients with CALR mutations (ET = 24, prePMF = 33, and overt PMF = 20). Targeted NGS using a 38-gene panel was performed to evaluate the variant allele frequency (VAF) of CALR type I/type II mutations and assess the molecular landscape of nondriver gene mutations.Results A lower VAF of type I vs. type II was observed in CALR-mutant ET, prePMF and overt PMF, and a higher frequency of type I vs. type II was found in CALR-mutant overt PMF. Additional somatic mutations were indicated to be useful in understanding the pathogenesis of MPN. In this study, the mutation landscape was more complex in overt PMF than in ET or in prePMF. Mutations in epigenetic regulators (ASXL1, EZH2 and TET2) were more common in overt PMF.Conclusions The two different subtypes of CALR mutations may have different impacts on MPN. A lower VAF of CALR type I indicates a greater contribution to disease progression in MPN, and increased nondriver mutations may be important in myelofibrosis progression.https://www.tandfonline.com/doi/10.1080/16078454.2022.2107888CALRvariant allele frequencynon-driver mutationessential thrombocythaemiaprimary myelofibrosis |
spellingShingle | Yuxia Pan Xingzhe Wang Shupeng Wen Xiaojun Liu Lin Yang Jianmin Luo The different variant allele frequencies of type I/type II mutations and the distinct molecular landscapes in CALR-mutant essential thrombocythaemia and primary myelofibrosis Hematology CALR variant allele frequency non-driver mutation essential thrombocythaemia primary myelofibrosis |
title | The different variant allele frequencies of type I/type II mutations and the distinct molecular landscapes in CALR-mutant essential thrombocythaemia and primary myelofibrosis |
title_full | The different variant allele frequencies of type I/type II mutations and the distinct molecular landscapes in CALR-mutant essential thrombocythaemia and primary myelofibrosis |
title_fullStr | The different variant allele frequencies of type I/type II mutations and the distinct molecular landscapes in CALR-mutant essential thrombocythaemia and primary myelofibrosis |
title_full_unstemmed | The different variant allele frequencies of type I/type II mutations and the distinct molecular landscapes in CALR-mutant essential thrombocythaemia and primary myelofibrosis |
title_short | The different variant allele frequencies of type I/type II mutations and the distinct molecular landscapes in CALR-mutant essential thrombocythaemia and primary myelofibrosis |
title_sort | different variant allele frequencies of type i type ii mutations and the distinct molecular landscapes in calr mutant essential thrombocythaemia and primary myelofibrosis |
topic | CALR variant allele frequency non-driver mutation essential thrombocythaemia primary myelofibrosis |
url | https://www.tandfonline.com/doi/10.1080/16078454.2022.2107888 |
work_keys_str_mv | AT yuxiapan thedifferentvariantallelefrequenciesoftypeitypeiimutationsandthedistinctmolecularlandscapesincalrmutantessentialthrombocythaemiaandprimarymyelofibrosis AT xingzhewang thedifferentvariantallelefrequenciesoftypeitypeiimutationsandthedistinctmolecularlandscapesincalrmutantessentialthrombocythaemiaandprimarymyelofibrosis AT shupengwen thedifferentvariantallelefrequenciesoftypeitypeiimutationsandthedistinctmolecularlandscapesincalrmutantessentialthrombocythaemiaandprimarymyelofibrosis AT xiaojunliu thedifferentvariantallelefrequenciesoftypeitypeiimutationsandthedistinctmolecularlandscapesincalrmutantessentialthrombocythaemiaandprimarymyelofibrosis AT linyang thedifferentvariantallelefrequenciesoftypeitypeiimutationsandthedistinctmolecularlandscapesincalrmutantessentialthrombocythaemiaandprimarymyelofibrosis AT jianminluo thedifferentvariantallelefrequenciesoftypeitypeiimutationsandthedistinctmolecularlandscapesincalrmutantessentialthrombocythaemiaandprimarymyelofibrosis AT yuxiapan differentvariantallelefrequenciesoftypeitypeiimutationsandthedistinctmolecularlandscapesincalrmutantessentialthrombocythaemiaandprimarymyelofibrosis AT xingzhewang differentvariantallelefrequenciesoftypeitypeiimutationsandthedistinctmolecularlandscapesincalrmutantessentialthrombocythaemiaandprimarymyelofibrosis AT shupengwen differentvariantallelefrequenciesoftypeitypeiimutationsandthedistinctmolecularlandscapesincalrmutantessentialthrombocythaemiaandprimarymyelofibrosis AT xiaojunliu differentvariantallelefrequenciesoftypeitypeiimutationsandthedistinctmolecularlandscapesincalrmutantessentialthrombocythaemiaandprimarymyelofibrosis AT linyang differentvariantallelefrequenciesoftypeitypeiimutationsandthedistinctmolecularlandscapesincalrmutantessentialthrombocythaemiaandprimarymyelofibrosis AT jianminluo differentvariantallelefrequenciesoftypeitypeiimutationsandthedistinctmolecularlandscapesincalrmutantessentialthrombocythaemiaandprimarymyelofibrosis |