Exploring the Diversity of Field Strains of Brucella abortus Biovar 3 Isolated in West Africa
Brucellosis is one of the most widespread bacterial zoonotic diseases in the world, affecting both humans and domestic and wild animals. Identification and biotyping of field strains of Brucella are of key importance for a better knowledge of the epidemiology of brucellosis, for identifying appropri...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01232/full |
| _version_ | 1819143052450594816 |
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| author | Moussa Sanogo David Fretin Eric Thys Claude Saegerman |
| author_facet | Moussa Sanogo David Fretin Eric Thys Claude Saegerman |
| author_sort | Moussa Sanogo |
| collection | DOAJ |
| description | Brucellosis is one of the most widespread bacterial zoonotic diseases in the world, affecting both humans and domestic and wild animals. Identification and biotyping of field strains of Brucella are of key importance for a better knowledge of the epidemiology of brucellosis, for identifying appropriate antigens, for managing disease outbreaks and for setting up efficient preventive and control programmes. Such data are required both at national and regional level to assess potential threats for public health. Highly discriminative genotyping methods such as the multiple locus variable number of tandem repeats analysis (MLVA) allow the comparison and assessment of genetic relatedness between field strains of Brucella within the same geographical area. In this study, MLVA biotyping data retrieved from the literature using a systematic review were compared using a clustering analysis and the Hunter-Gaston diversity index (HGDI). Thus, the analysis of the 42 MLVA genotyping results found in the literature on West Africa [i.e., from Ivory Coast (1), Niger (1), Nigeria (34), The Gambia (3), and Togo (3)] did not allow a complete assessment of the actual diversity among field strains of Brucella. However, it provided some preliminary indications on the co-existence of 25 distinct genotypes of Brucella abortus biovar 3 in this region with 19 genotypes from Nigeria, three from Togo and one from Ivory Coast, The Gambia, and Niger. The strong and urgent need for more sustainable molecular data on prevailing strains of Brucella in this sub-region of Africa and also on all susceptible species including humans is therefore highlighted. This remains a necessary stage to allow a comprehensive understanding of the relatedness between field strains of Brucella and the epidemiology of brucellosis within West Africa countries. |
| first_indexed | 2024-12-22T12:20:06Z |
| format | Article |
| id | doaj.art-4855627e8a7d470e80d8da62fa0cb8c5 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T12:20:06Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4855627e8a7d470e80d8da62fa0cb8c52022-12-21T18:26:00ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-06-01810.3389/fmicb.2017.01232243910Exploring the Diversity of Field Strains of Brucella abortus Biovar 3 Isolated in West AfricaMoussa Sanogo0David Fretin1Eric Thys2Claude Saegerman3Central Veterinary Laboratory of Bingerville, LANADABingerville, Ivory CoastDepartment of Bacteriology and Immunology, Veterinary and Agro-chemical Research CentreBrussels, BelgiumDepartment of Biomedical Sciences, Institute of Tropical MedicineAntwerp, BelgiumResearch Unit of Epidemiology and Risk Analysis Applied to Veterinary Sciences (UREAR-ULg), Faculty of Veterinary Medicine, Fundamental and Applied Research for Animal and Health Center, University of LiègeLiège, BelgiumBrucellosis is one of the most widespread bacterial zoonotic diseases in the world, affecting both humans and domestic and wild animals. Identification and biotyping of field strains of Brucella are of key importance for a better knowledge of the epidemiology of brucellosis, for identifying appropriate antigens, for managing disease outbreaks and for setting up efficient preventive and control programmes. Such data are required both at national and regional level to assess potential threats for public health. Highly discriminative genotyping methods such as the multiple locus variable number of tandem repeats analysis (MLVA) allow the comparison and assessment of genetic relatedness between field strains of Brucella within the same geographical area. In this study, MLVA biotyping data retrieved from the literature using a systematic review were compared using a clustering analysis and the Hunter-Gaston diversity index (HGDI). Thus, the analysis of the 42 MLVA genotyping results found in the literature on West Africa [i.e., from Ivory Coast (1), Niger (1), Nigeria (34), The Gambia (3), and Togo (3)] did not allow a complete assessment of the actual diversity among field strains of Brucella. However, it provided some preliminary indications on the co-existence of 25 distinct genotypes of Brucella abortus biovar 3 in this region with 19 genotypes from Nigeria, three from Togo and one from Ivory Coast, The Gambia, and Niger. The strong and urgent need for more sustainable molecular data on prevailing strains of Brucella in this sub-region of Africa and also on all susceptible species including humans is therefore highlighted. This remains a necessary stage to allow a comprehensive understanding of the relatedness between field strains of Brucella and the epidemiology of brucellosis within West Africa countries.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01232/fullmolecular epidemiologybrucellosisBrucellaBrucella abortus biovar 3biotypingMLVA |
| spellingShingle | Moussa Sanogo David Fretin Eric Thys Claude Saegerman Exploring the Diversity of Field Strains of Brucella abortus Biovar 3 Isolated in West Africa Frontiers in Microbiology molecular epidemiology brucellosis Brucella Brucella abortus biovar 3 biotyping MLVA |
| title | Exploring the Diversity of Field Strains of Brucella abortus Biovar 3 Isolated in West Africa |
| title_full | Exploring the Diversity of Field Strains of Brucella abortus Biovar 3 Isolated in West Africa |
| title_fullStr | Exploring the Diversity of Field Strains of Brucella abortus Biovar 3 Isolated in West Africa |
| title_full_unstemmed | Exploring the Diversity of Field Strains of Brucella abortus Biovar 3 Isolated in West Africa |
| title_short | Exploring the Diversity of Field Strains of Brucella abortus Biovar 3 Isolated in West Africa |
| title_sort | exploring the diversity of field strains of brucella abortus biovar 3 isolated in west africa |
| topic | molecular epidemiology brucellosis Brucella Brucella abortus biovar 3 biotyping MLVA |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01232/full |
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