CD9 predicts ETV6-RUNX1 in childhood B-cell precursor acute lymphoblastic leukemia
Introduction: The ETV6-RUNX1 is a fusion gene associated with a good outcome in B-cell precursor lymphoblastic leukemia. Objective: This study aimed to re-evaluate the CD9 cellular expression by flow cytometry (FC) as a possible tool to predict the presence of ETV6-RUNX1. Method: Childhood B-cell pr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-07-01
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| Series: | Hematology, Transfusion and Cell Therapy |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2531137919300318 |
| _version_ | 1818889316851515392 |
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| author | Caroline B. Blunck Eugênia Terra-Granado Elda P. Noronha Gabriel Wajnberg Fábio Passetti Maria S. Pombo-de-Oliveira Mariana Emerenciano |
| author_facet | Caroline B. Blunck Eugênia Terra-Granado Elda P. Noronha Gabriel Wajnberg Fábio Passetti Maria S. Pombo-de-Oliveira Mariana Emerenciano |
| author_sort | Caroline B. Blunck |
| collection | DOAJ |
| description | Introduction: The ETV6-RUNX1 is a fusion gene associated with a good outcome in B-cell precursor lymphoblastic leukemia. Objective: This study aimed to re-evaluate the CD9 cellular expression by flow cytometry (FC) as a possible tool to predict the presence of ETV6-RUNX1. Method: Childhood B-cell precursor lymphoblastic leukemia cases were included (n = 186). The percentage of CD9-labeled cells and the median fluorescence intensity ratio were used for correlation with the molecular tests. Receiver Operating Characteristic curves were performed to determine the likelihood of the CD9 expression predicting ETV6-RUNX1. Results: The ETV6-RUNX1 was found in 44/186 (23.6%) cases. Data analysis revealed that the best cutoff for CD9 percentage was 64%, with an accuracy of 0.84, whereas the best cutoff for CD9 median fluorescence intensity ratio was 12.52, with an accuracy of 0.80. A strong association was observed between the level of CD9 expression and the presence of ETV6-RUNX1. Conclusion: These data confirm that the CD9 expression could be used for risk stratification in clinical practice. Keywords: Acute lymphoblastic leukemia, Biomarker, CD9, ETV6-RUNX1, Flow cytometry |
| first_indexed | 2024-12-19T17:07:05Z |
| format | Article |
| id | doaj.art-485a930cff6f4a1c83354f01835a6f36 |
| institution | Directory Open Access Journal |
| issn | 2531-1379 |
| language | English |
| last_indexed | 2024-12-19T17:07:05Z |
| publishDate | 2019-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Hematology, Transfusion and Cell Therapy |
| spelling | doaj.art-485a930cff6f4a1c83354f01835a6f362022-12-21T20:13:07ZengElsevierHematology, Transfusion and Cell Therapy2531-13792019-07-01413205211CD9 predicts ETV6-RUNX1 in childhood B-cell precursor acute lymphoblastic leukemiaCaroline B. Blunck0Eugênia Terra-Granado1Elda P. Noronha2Gabriel Wajnberg3Fábio Passetti4Maria S. Pombo-de-Oliveira5Mariana Emerenciano6Instituto Nacional de Câncer (INCA), Rio de Janeiro, RJ, BrazilInstituto Nacional de Câncer (INCA), Rio de Janeiro, RJ, BrazilInstituto Nacional de Câncer (INCA), Rio de Janeiro, RJ, BrazilInstituto Nacional de Câncer (INCA), Rio de Janeiro, RJ, Brazil; Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, RJ, Brazil; Atlantic Cancer Research Institute, Moncton, CanadaInstituto Nacional de Câncer (INCA), Rio de Janeiro, RJ, Brazil; Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, RJ, BrazilInstituto Nacional de Câncer (INCA), Rio de Janeiro, RJ, BrazilInstituto Nacional de Câncer (INCA), Rio de Janeiro, RJ, Brazil; Corresponding author at: Research Center, Instituto Nacional de Câncer (INCA), Rua André Cavalcanti, 37, Rio de Janeiro CEP: 20231-050, RJ, Brazil.Introduction: The ETV6-RUNX1 is a fusion gene associated with a good outcome in B-cell precursor lymphoblastic leukemia. Objective: This study aimed to re-evaluate the CD9 cellular expression by flow cytometry (FC) as a possible tool to predict the presence of ETV6-RUNX1. Method: Childhood B-cell precursor lymphoblastic leukemia cases were included (n = 186). The percentage of CD9-labeled cells and the median fluorescence intensity ratio were used for correlation with the molecular tests. Receiver Operating Characteristic curves were performed to determine the likelihood of the CD9 expression predicting ETV6-RUNX1. Results: The ETV6-RUNX1 was found in 44/186 (23.6%) cases. Data analysis revealed that the best cutoff for CD9 percentage was 64%, with an accuracy of 0.84, whereas the best cutoff for CD9 median fluorescence intensity ratio was 12.52, with an accuracy of 0.80. A strong association was observed between the level of CD9 expression and the presence of ETV6-RUNX1. Conclusion: These data confirm that the CD9 expression could be used for risk stratification in clinical practice. Keywords: Acute lymphoblastic leukemia, Biomarker, CD9, ETV6-RUNX1, Flow cytometryhttp://www.sciencedirect.com/science/article/pii/S2531137919300318 |
| spellingShingle | Caroline B. Blunck Eugênia Terra-Granado Elda P. Noronha Gabriel Wajnberg Fábio Passetti Maria S. Pombo-de-Oliveira Mariana Emerenciano CD9 predicts ETV6-RUNX1 in childhood B-cell precursor acute lymphoblastic leukemia Hematology, Transfusion and Cell Therapy |
| title | CD9 predicts ETV6-RUNX1 in childhood B-cell precursor acute lymphoblastic leukemia |
| title_full | CD9 predicts ETV6-RUNX1 in childhood B-cell precursor acute lymphoblastic leukemia |
| title_fullStr | CD9 predicts ETV6-RUNX1 in childhood B-cell precursor acute lymphoblastic leukemia |
| title_full_unstemmed | CD9 predicts ETV6-RUNX1 in childhood B-cell precursor acute lymphoblastic leukemia |
| title_short | CD9 predicts ETV6-RUNX1 in childhood B-cell precursor acute lymphoblastic leukemia |
| title_sort | cd9 predicts etv6 runx1 in childhood b cell precursor acute lymphoblastic leukemia |
| url | http://www.sciencedirect.com/science/article/pii/S2531137919300318 |
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