Sensitivity of <i>Hydra vulgaris</i> to Nanosilver for Environmental Applications
Nanosilver applications, including sensing and water treatment, have significantly increased in recent years, although safety for humans and the environment is still under debate. Here, we tested the environmental safety of a novel formulation of silver nanoparticles functionalized with citrate and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/10/11/695 |
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| author | Arianna Bellingeri Chiara Battocchio Claudia Faleri Giuseppe Protano Iole Venditti Ilaria Corsi |
| author_facet | Arianna Bellingeri Chiara Battocchio Claudia Faleri Giuseppe Protano Iole Venditti Ilaria Corsi |
| author_sort | Arianna Bellingeri |
| collection | DOAJ |
| description | Nanosilver applications, including sensing and water treatment, have significantly increased in recent years, although safety for humans and the environment is still under debate. Here, we tested the environmental safety of a novel formulation of silver nanoparticles functionalized with citrate and L-cysteine (AgNPcitLcys) on freshwater cnidarian <i>Hydra vulgaris</i> as an emerging ecotoxicological model for the safety of engineered nanomaterials. AgNPcitLcys behavior was characterized by dynamic light scattering (DLS), while Ag release was measured by inductively coupled plasma mass spectrometry (ICP-MS). <i>H. vulgaris</i> (<i>n</i> = 12) subjects were evaluated for morphological aberration after 96 h of exposure and regeneration ability after 96 h and 7 days of exposure, after which the predatory ability was also assessed. The results show a low dissolution of AgNPcitLcys in <i>Hydra</i> medium (max 0.146% of nominal AgNPcitLcys concentration) and highlight a lack of ecotoxicological effects, both on morphology and regeneration, confirming the protective role of the double coating against AgNP biological effects. Predatory ability evaluation suggests a mild impairment of the entangling capacity or of the functionality of the tentacles, as the number of preys killed but not ingested was higher than the controls in all exposed animals. While their long-term sub-lethal effects still need to be further evaluated on <i>H. vulgaris</i>, AgNPcitLcys appears to be a promising tool for environmental applications, for instance, for water treatment and sensing. |
| first_indexed | 2024-03-09T17:57:02Z |
| format | Article |
| id | doaj.art-485c3512a3bc42b082d684a7a11ca184 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-09T17:57:02Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-485c3512a3bc42b082d684a7a11ca1842023-11-24T10:14:52ZengMDPI AGToxics2305-63042022-11-01101169510.3390/toxics10110695Sensitivity of <i>Hydra vulgaris</i> to Nanosilver for Environmental ApplicationsArianna Bellingeri0Chiara Battocchio1Claudia Faleri2Giuseppe Protano3Iole Venditti4Ilaria Corsi5Department of Physical, Earth and Environmental Sciences, University of Siena, 53100 Siena, ItalyDepartment of Sciences, Roma Tre University of Rome, 00146 Rome, ItalyDepartment of Life Sciences, University of Siena, 53100 Siena, ItalyDepartment of Physical, Earth and Environmental Sciences, University of Siena, 53100 Siena, ItalyDepartment of Sciences, Roma Tre University of Rome, 00146 Rome, ItalyDepartment of Physical, Earth and Environmental Sciences, University of Siena, 53100 Siena, ItalyNanosilver applications, including sensing and water treatment, have significantly increased in recent years, although safety for humans and the environment is still under debate. Here, we tested the environmental safety of a novel formulation of silver nanoparticles functionalized with citrate and L-cysteine (AgNPcitLcys) on freshwater cnidarian <i>Hydra vulgaris</i> as an emerging ecotoxicological model for the safety of engineered nanomaterials. AgNPcitLcys behavior was characterized by dynamic light scattering (DLS), while Ag release was measured by inductively coupled plasma mass spectrometry (ICP-MS). <i>H. vulgaris</i> (<i>n</i> = 12) subjects were evaluated for morphological aberration after 96 h of exposure and regeneration ability after 96 h and 7 days of exposure, after which the predatory ability was also assessed. The results show a low dissolution of AgNPcitLcys in <i>Hydra</i> medium (max 0.146% of nominal AgNPcitLcys concentration) and highlight a lack of ecotoxicological effects, both on morphology and regeneration, confirming the protective role of the double coating against AgNP biological effects. Predatory ability evaluation suggests a mild impairment of the entangling capacity or of the functionality of the tentacles, as the number of preys killed but not ingested was higher than the controls in all exposed animals. While their long-term sub-lethal effects still need to be further evaluated on <i>H. vulgaris</i>, AgNPcitLcys appears to be a promising tool for environmental applications, for instance, for water treatment and sensing.https://www.mdpi.com/2305-6304/10/11/695nanosilverecotoxicitycnidarian<i>Hydra</i>sensingwater treatment |
| spellingShingle | Arianna Bellingeri Chiara Battocchio Claudia Faleri Giuseppe Protano Iole Venditti Ilaria Corsi Sensitivity of <i>Hydra vulgaris</i> to Nanosilver for Environmental Applications Toxics nanosilver ecotoxicity cnidarian <i>Hydra</i> sensing water treatment |
| title | Sensitivity of <i>Hydra vulgaris</i> to Nanosilver for Environmental Applications |
| title_full | Sensitivity of <i>Hydra vulgaris</i> to Nanosilver for Environmental Applications |
| title_fullStr | Sensitivity of <i>Hydra vulgaris</i> to Nanosilver for Environmental Applications |
| title_full_unstemmed | Sensitivity of <i>Hydra vulgaris</i> to Nanosilver for Environmental Applications |
| title_short | Sensitivity of <i>Hydra vulgaris</i> to Nanosilver for Environmental Applications |
| title_sort | sensitivity of i hydra vulgaris i to nanosilver for environmental applications |
| topic | nanosilver ecotoxicity cnidarian <i>Hydra</i> sensing water treatment |
| url | https://www.mdpi.com/2305-6304/10/11/695 |
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