Characterization of Rhodopseudomonas palustris population dynamics on tobacco phyllosphere and induction of plant resistance to Tobacco mosaic virus
Summary Although many biocontrol bacteria can be used to improve plant tolerance to stresses and to promote plant growth, the hostile environmental conditions on plant phyllosphere and the limited knowledge on bacterial colonization on plant phyllosphere minimized the beneficial effects produced by...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-11-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13486 |
| _version_ | 1818494411707777024 |
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| author | Pin Su Deyong Zhang Zhuo Zhang Ang Chen Muhammad Rizwan Hamid Chenggang Li Jiao Du Ju'e Cheng Xinqiu Tan Limin Zhen Zhongying Zhai Wen Tang Jin Chen Xuguo Zhou Yong Liu |
| author_facet | Pin Su Deyong Zhang Zhuo Zhang Ang Chen Muhammad Rizwan Hamid Chenggang Li Jiao Du Ju'e Cheng Xinqiu Tan Limin Zhen Zhongying Zhai Wen Tang Jin Chen Xuguo Zhou Yong Liu |
| author_sort | Pin Su |
| collection | DOAJ |
| description | Summary Although many biocontrol bacteria can be used to improve plant tolerance to stresses and to promote plant growth, the hostile environmental conditions on plant phyllosphere and the limited knowledge on bacterial colonization on plant phyllosphere minimized the beneficial effects produced by the biocontrol bacteria. Rhodopseudomonas palustris strain GJ‐22 is known as a phyllosphere biocontrol agent. In this paper, we described detailed processes of strain GJ‐22 colony establishment at various colonization stages. Four different types of bacterial colonies, Type 1, scattered single cells; Type 2, small cell clusters; Type 3, small cell aggregates; and Type 4, large cell aggregates, were observed in the course of bacterial colonization. We categorized bacterial colonization into four phases, which were, Phase I: bacterial colony exists as Type 1 and cell population reduced quickly; Phase II: Type 1 evolved into Type 2 and cell population remained steady; Phase III: Type 3 arose and replaced Type 2, and cell population expanded slowly; and Phase IV: Type 3 matured into Type 4 and cell population increased quickly. We have shown that the preferable location sites of bacterial aggregates on leaf phyllosphere are grooves between plant epidermal cells. Analyses of expressions of plant defence‐related genes showed that, starting from Phase III, bacterial cells in the Type 3 and Type 4 colonies produced unidentified signals to induce host defence against Tobacco mosaic virus infection. In addition, we determined the crucial role of aggregates formation of GJ‐22 cell on plant phyllosphere in terms of bacterial cell stress tolerance and ISR (induced systemic resistance) priming. To our knowledge, this is the first report focused on the colonization process of a phyllosphere biocontrol agent and gave a clear description on the morphological shift of bacterial colony on phyllosphere. |
| first_indexed | 2024-12-10T18:05:47Z |
| format | Article |
| id | doaj.art-4861071daa804077b876365546a7773c |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-10T18:05:47Z |
| publishDate | 2019-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-4861071daa804077b876365546a7773c2022-12-22T01:38:36ZengWileyMicrobial Biotechnology1751-79152019-11-011261453146310.1111/1751-7915.13486Characterization of Rhodopseudomonas palustris population dynamics on tobacco phyllosphere and induction of plant resistance to Tobacco mosaic virusPin Su0Deyong Zhang1Zhuo Zhang2Ang Chen3Muhammad Rizwan Hamid4Chenggang Li5Jiao Du6Ju'e Cheng7Xinqiu Tan8Limin Zhen9Zhongying Zhai10Wen Tang11Jin Chen12Xuguo Zhou13Yong Liu14Hunan Plant Protection Institute Hunan Academy of Agricultural Sciences Changsha 410125 ChinaHunan Plant Protection Institute Hunan Academy of Agricultural Sciences Changsha 410125 ChinaHunan Plant Protection Institute Hunan Academy of Agricultural Sciences Changsha 410125 ChinaHunan Plant Protection Institute Hunan Academy of Agricultural Sciences Changsha 410125 ChinaHunan Plant Protection Institute Hunan Academy of Agricultural Sciences Changsha 410125 ChinaHunan Plant Protection Institute Hunan Academy of Agricultural Sciences Changsha 410125 ChinaHunan Plant Protection Institute Hunan Academy of Agricultural Sciences Changsha 410125 ChinaHunan Plant Protection Institute Hunan Academy of Agricultural Sciences Changsha 410125 ChinaHunan Plant Protection Institute Hunan Academy of Agricultural Sciences Changsha 410125 ChinaHunan Plant Protection Institute Hunan Academy of Agricultural Sciences Changsha 410125 ChinaHunan Plant Protection Institute Hunan Academy of Agricultural Sciences Changsha 410125 ChinaHunan Plant Protection Institute Hunan Academy of Agricultural Sciences Changsha 410125 ChinaCollege of Bioscience and Biotechnology Hunan Agricultural University Changsha 410128 ChinaDepartment of Entomology University of Kentucky Lexington KY 40546 USAHunan Plant Protection Institute Hunan Academy of Agricultural Sciences Changsha 410125 ChinaSummary Although many biocontrol bacteria can be used to improve plant tolerance to stresses and to promote plant growth, the hostile environmental conditions on plant phyllosphere and the limited knowledge on bacterial colonization on plant phyllosphere minimized the beneficial effects produced by the biocontrol bacteria. Rhodopseudomonas palustris strain GJ‐22 is known as a phyllosphere biocontrol agent. In this paper, we described detailed processes of strain GJ‐22 colony establishment at various colonization stages. Four different types of bacterial colonies, Type 1, scattered single cells; Type 2, small cell clusters; Type 3, small cell aggregates; and Type 4, large cell aggregates, were observed in the course of bacterial colonization. We categorized bacterial colonization into four phases, which were, Phase I: bacterial colony exists as Type 1 and cell population reduced quickly; Phase II: Type 1 evolved into Type 2 and cell population remained steady; Phase III: Type 3 arose and replaced Type 2, and cell population expanded slowly; and Phase IV: Type 3 matured into Type 4 and cell population increased quickly. We have shown that the preferable location sites of bacterial aggregates on leaf phyllosphere are grooves between plant epidermal cells. Analyses of expressions of plant defence‐related genes showed that, starting from Phase III, bacterial cells in the Type 3 and Type 4 colonies produced unidentified signals to induce host defence against Tobacco mosaic virus infection. In addition, we determined the crucial role of aggregates formation of GJ‐22 cell on plant phyllosphere in terms of bacterial cell stress tolerance and ISR (induced systemic resistance) priming. To our knowledge, this is the first report focused on the colonization process of a phyllosphere biocontrol agent and gave a clear description on the morphological shift of bacterial colony on phyllosphere.https://doi.org/10.1111/1751-7915.13486 |
| spellingShingle | Pin Su Deyong Zhang Zhuo Zhang Ang Chen Muhammad Rizwan Hamid Chenggang Li Jiao Du Ju'e Cheng Xinqiu Tan Limin Zhen Zhongying Zhai Wen Tang Jin Chen Xuguo Zhou Yong Liu Characterization of Rhodopseudomonas palustris population dynamics on tobacco phyllosphere and induction of plant resistance to Tobacco mosaic virus Microbial Biotechnology |
| title | Characterization of Rhodopseudomonas palustris population dynamics on tobacco phyllosphere and induction of plant resistance to Tobacco mosaic virus |
| title_full | Characterization of Rhodopseudomonas palustris population dynamics on tobacco phyllosphere and induction of plant resistance to Tobacco mosaic virus |
| title_fullStr | Characterization of Rhodopseudomonas palustris population dynamics on tobacco phyllosphere and induction of plant resistance to Tobacco mosaic virus |
| title_full_unstemmed | Characterization of Rhodopseudomonas palustris population dynamics on tobacco phyllosphere and induction of plant resistance to Tobacco mosaic virus |
| title_short | Characterization of Rhodopseudomonas palustris population dynamics on tobacco phyllosphere and induction of plant resistance to Tobacco mosaic virus |
| title_sort | characterization of rhodopseudomonas palustris population dynamics on tobacco phyllosphere and induction of plant resistance to tobacco mosaic virus |
| url | https://doi.org/10.1111/1751-7915.13486 |
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