Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva
Magnetic nanoparticles (MNPs) were synthesized using the colloidal co-precipitation method and further coated with silica using the Stöber process. These were functionalized with carboxylic and amine functionalities for further covalent immobilization of antibodies on these MNPs. The procedure for c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/17/3968 |
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| author | Arpita Saha Hamdi Ben Halima Abhishek Saini Juan Gallardo-Gonzalez Nadia Zine Clara Viñas Abdelhamid Elaissari Abdelhamid Errachid Francesc Teixidor |
| author_facet | Arpita Saha Hamdi Ben Halima Abhishek Saini Juan Gallardo-Gonzalez Nadia Zine Clara Viñas Abdelhamid Elaissari Abdelhamid Errachid Francesc Teixidor |
| author_sort | Arpita Saha |
| collection | DOAJ |
| description | Magnetic nanoparticles (MNPs) were synthesized using the colloidal co-precipitation method and further coated with silica using the Stöber process. These were functionalized with carboxylic and amine functionalities for further covalent immobilization of antibodies on these MNPs. The procedure for covalent immobilization of antibodies on MNPs was developed using 1-ethyl-3-(dimethylaminopropyl)carbodiimide (EDC) and <i>N</i>-hydroxysuccinimide (NHS). The evaluation of the efficiency of the coupling reaction was carried out by UV-vis spectrophotometry. The developed antibodies coupled to MNPs were tested for the pre-concentration of two biomarkers tumor necrosis factor alpha (TNF-α) and Interleukin-10 (IL-10). Both biomarkers were assessed in the matrix based on phosphate-buffered saline solution (PBS) and artificial saliva (AS) to carry out the demonstration of the format assay. Supernatants were used to determine the number of free biomarkers for both studies. Reduction of the nonspecific saliva protein adsorption on the surface of the complex antibodies-MNPs to levels low enough to allow the detection of biomarkers in complex media has been achieved. |
| first_indexed | 2024-03-10T16:40:40Z |
| format | Article |
| id | doaj.art-486647fa00e64a5ca7fc7623e75251c2 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:40:40Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-486647fa00e64a5ca7fc7623e75251c22023-11-20T12:00:12ZengMDPI AGMolecules1420-30492020-08-012517396810.3390/molecules25173968Magnetic Nanoparticles Fishing for Biomarkers in Artificial SalivaArpita Saha0Hamdi Ben Halima1Abhishek Saini2Juan Gallardo-Gonzalez3Nadia Zine4Clara Viñas5Abdelhamid Elaissari6Abdelhamid Errachid7Francesc Teixidor8Inorganic Materials Laboratory, Institut de Ciencia de Materials de Barcelona (ICMAB-CSIC), Campus de la UAB, 08193 Bellaterra, SpainMicro & Nanobiotechnology Laboratory, Université de Lyon, CNRS, University Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, FranceInorganic Materials Laboratory, Institut de Ciencia de Materials de Barcelona (ICMAB-CSIC), Campus de la UAB, 08193 Bellaterra, SpainMicro & Nanobiotechnology Laboratory, Université de Lyon, CNRS, University Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, FranceMicro & Nanobiotechnology Laboratory, Université de Lyon, CNRS, University Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, FranceInorganic Materials Laboratory, Institut de Ciencia de Materials de Barcelona (ICMAB-CSIC), Campus de la UAB, 08193 Bellaterra, SpainLAGEPP-UMR 5007, CNRS, University Claude Bernard Lyon-1, University of Lyon, F-69622 Lyon, FranceMicro & Nanobiotechnology Laboratory, Université de Lyon, CNRS, University Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, FranceInorganic Materials Laboratory, Institut de Ciencia de Materials de Barcelona (ICMAB-CSIC), Campus de la UAB, 08193 Bellaterra, SpainMagnetic nanoparticles (MNPs) were synthesized using the colloidal co-precipitation method and further coated with silica using the Stöber process. These were functionalized with carboxylic and amine functionalities for further covalent immobilization of antibodies on these MNPs. The procedure for covalent immobilization of antibodies on MNPs was developed using 1-ethyl-3-(dimethylaminopropyl)carbodiimide (EDC) and <i>N</i>-hydroxysuccinimide (NHS). The evaluation of the efficiency of the coupling reaction was carried out by UV-vis spectrophotometry. The developed antibodies coupled to MNPs were tested for the pre-concentration of two biomarkers tumor necrosis factor alpha (TNF-α) and Interleukin-10 (IL-10). Both biomarkers were assessed in the matrix based on phosphate-buffered saline solution (PBS) and artificial saliva (AS) to carry out the demonstration of the format assay. Supernatants were used to determine the number of free biomarkers for both studies. Reduction of the nonspecific saliva protein adsorption on the surface of the complex antibodies-MNPs to levels low enough to allow the detection of biomarkers in complex media has been achieved.https://www.mdpi.com/1420-3049/25/17/3968immobilization of antibodiesIL-10magnetic nanoparticlespre-concentration of antigenssaliva matrixTNF-α |
| spellingShingle | Arpita Saha Hamdi Ben Halima Abhishek Saini Juan Gallardo-Gonzalez Nadia Zine Clara Viñas Abdelhamid Elaissari Abdelhamid Errachid Francesc Teixidor Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva Molecules immobilization of antibodies IL-10 magnetic nanoparticles pre-concentration of antigens saliva matrix TNF-α |
| title | Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva |
| title_full | Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva |
| title_fullStr | Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva |
| title_full_unstemmed | Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva |
| title_short | Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva |
| title_sort | magnetic nanoparticles fishing for biomarkers in artificial saliva |
| topic | immobilization of antibodies IL-10 magnetic nanoparticles pre-concentration of antigens saliva matrix TNF-α |
| url | https://www.mdpi.com/1420-3049/25/17/3968 |
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