A High-Throughput and Uniform Amplification Method for Cell Spheroids
Cell culture is an important life science technology. Compared with the traditional two-dimensional cell culture, three-dimensional cell culture can simulate the natural environment and structure specificity of cell growth in vivo. As such, it has become a research hotspot. The existing three-dimens...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/10/1645 |
| _version_ | 1797471232137887744 |
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| author | Liyuan Liu Haixia Liu Xiaowen Huang Xiaoli Liu Chengyun Zheng |
| author_facet | Liyuan Liu Haixia Liu Xiaowen Huang Xiaoli Liu Chengyun Zheng |
| author_sort | Liyuan Liu |
| collection | DOAJ |
| description | Cell culture is an important life science technology. Compared with the traditional two-dimensional cell culture, three-dimensional cell culture can simulate the natural environment and structure specificity of cell growth in vivo. As such, it has become a research hotspot. The existing three-dimensional cell culture techniques include the hanging drop method, spinner flask method, etc., making it difficult to ensure uniform morphology of the obtained cell spheroids while performing high-throughput. Here, we report a method for amplifying cell spheroids with the advantages of quickly enlarging the culture scale and obtaining cell spheroids with uniform morphology and a survival rate of over 95%. Technically, it is easy to operate and convenient to change substances. These results indicate that this method has the potential to become a promising approach for cell–cell, cell–stroma, cell–organ mutual interaction research, tissue engineering, and anti-cancer drug screening. |
| first_indexed | 2024-03-09T19:46:14Z |
| format | Article |
| id | doaj.art-4867a92b1fed4d63941e54e9adb267e0 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T19:46:14Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-4867a92b1fed4d63941e54e9adb267e02023-11-24T01:22:10ZengMDPI AGMicromachines2072-666X2022-09-011310164510.3390/mi13101645A High-Throughput and Uniform Amplification Method for Cell SpheroidsLiyuan Liu0Haixia Liu1Xiaowen Huang2Xiaoli Liu3Chengyun Zheng4Department of Hematology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250033, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, ChinaDepartment of Hematology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250033, ChinaDepartment of Hematology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250033, ChinaCell culture is an important life science technology. Compared with the traditional two-dimensional cell culture, three-dimensional cell culture can simulate the natural environment and structure specificity of cell growth in vivo. As such, it has become a research hotspot. The existing three-dimensional cell culture techniques include the hanging drop method, spinner flask method, etc., making it difficult to ensure uniform morphology of the obtained cell spheroids while performing high-throughput. Here, we report a method for amplifying cell spheroids with the advantages of quickly enlarging the culture scale and obtaining cell spheroids with uniform morphology and a survival rate of over 95%. Technically, it is easy to operate and convenient to change substances. These results indicate that this method has the potential to become a promising approach for cell–cell, cell–stroma, cell–organ mutual interaction research, tissue engineering, and anti-cancer drug screening.https://www.mdpi.com/2072-666X/13/10/1645cell culture3D cell spheroidshigh-throughputuniform shapedrug screening |
| spellingShingle | Liyuan Liu Haixia Liu Xiaowen Huang Xiaoli Liu Chengyun Zheng A High-Throughput and Uniform Amplification Method for Cell Spheroids Micromachines cell culture 3D cell spheroids high-throughput uniform shape drug screening |
| title | A High-Throughput and Uniform Amplification Method for Cell Spheroids |
| title_full | A High-Throughput and Uniform Amplification Method for Cell Spheroids |
| title_fullStr | A High-Throughput and Uniform Amplification Method for Cell Spheroids |
| title_full_unstemmed | A High-Throughput and Uniform Amplification Method for Cell Spheroids |
| title_short | A High-Throughput and Uniform Amplification Method for Cell Spheroids |
| title_sort | high throughput and uniform amplification method for cell spheroids |
| topic | cell culture 3D cell spheroids high-throughput uniform shape drug screening |
| url | https://www.mdpi.com/2072-666X/13/10/1645 |
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