Genome-wide identification, expression and function analysis of the MTP gene family in tulip (Tulipa gesneriana)
Currently, soil heavy metal contamination is a severe issue, particularly with Cd pollution. The metal tolerance protein (MTP) proteins, as plant divalent cation transporters, play a crucial role in the transport and tolerance of heavy metals in plants. This study conducted comprehensive identificat...
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| Format: | Article |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1346255/full |
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| author | Jiaojiao Lu Jiaojiao Lu Guimei Xing Guimei Xing Yanqiu Zhang Huihua Zhang Tianyu Wu Zengzhi Tian Lianwei Qu Lianwei Qu |
| author_facet | Jiaojiao Lu Jiaojiao Lu Guimei Xing Guimei Xing Yanqiu Zhang Huihua Zhang Tianyu Wu Zengzhi Tian Lianwei Qu Lianwei Qu |
| author_sort | Jiaojiao Lu |
| collection | DOAJ |
| description | Currently, soil heavy metal contamination is a severe issue, particularly with Cd pollution. The metal tolerance protein (MTP) proteins, as plant divalent cation transporters, play a crucial role in the transport and tolerance of heavy metals in plants. This study conducted comprehensive identification and characterization of the MTP gene family in the tulip. A total of 11 TgMTP genes were identified and phylogenetically classified into three subfamilies. Conserved motif and gene structure analyses unveiled commonalities and variations among subfamily members. Expression profiling demonstrated several TgMTPs were markedly upregulated under Cd exposure, including the TgMTP7.1. Heterologous expression in yeast validated that TgMTP7.1 could ameliorate Cd sensitivity and enhance its tolerance. These results provide primary insights into the MTP gene family in tulip. Phylogenetic relationships and functional analyses establish a framework for elucidating the transporters and molecular mechanisms governing Cd accumulation and distribution in tulip. Key TgMTPs identified, exemplified by TgMTP7.1, may illuminate molecular breeding efforts aimed at developing Cd-tolerant cultivars for the remediation of soil Cd contamination. |
| first_indexed | 2024-03-07T23:50:57Z |
| format | Article |
| id | doaj.art-48682e49f6774dfb92c1d93e4bc6c317 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-07T23:50:57Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-48682e49f6774dfb92c1d93e4bc6c3172024-02-19T04:56:16ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-02-011510.3389/fpls.2024.13462551346255Genome-wide identification, expression and function analysis of the MTP gene family in tulip (Tulipa gesneriana)Jiaojiao Lu0Jiaojiao Lu1Guimei Xing2Guimei Xing3Yanqiu Zhang4Huihua Zhang5Tianyu Wu6Zengzhi Tian7Lianwei Qu8Lianwei Qu9Institute of Floriculture, Liaoning Academy of Agriculture Sciences, Shenyang, Liaoning, ChinaLiaoning Provincial Key Laboratory of Floriculture, Shenyang, Liaoning, ChinaInstitute of Floriculture, Liaoning Academy of Agriculture Sciences, Shenyang, Liaoning, ChinaLiaoning Provincial Key Laboratory of Floriculture, Shenyang, Liaoning, ChinaInstitute of Floriculture, Liaoning Academy of Agriculture Sciences, Shenyang, Liaoning, ChinaInstitute of Floriculture, Liaoning Academy of Agriculture Sciences, Shenyang, Liaoning, ChinaInstitute of Floriculture, Liaoning Academy of Agriculture Sciences, Shenyang, Liaoning, ChinaInstitute of Floriculture, Liaoning Academy of Agriculture Sciences, Shenyang, Liaoning, ChinaInstitute of Floriculture, Liaoning Academy of Agriculture Sciences, Shenyang, Liaoning, ChinaLiaoning Provincial Key Laboratory of Floriculture, Shenyang, Liaoning, ChinaCurrently, soil heavy metal contamination is a severe issue, particularly with Cd pollution. The metal tolerance protein (MTP) proteins, as plant divalent cation transporters, play a crucial role in the transport and tolerance of heavy metals in plants. This study conducted comprehensive identification and characterization of the MTP gene family in the tulip. A total of 11 TgMTP genes were identified and phylogenetically classified into three subfamilies. Conserved motif and gene structure analyses unveiled commonalities and variations among subfamily members. Expression profiling demonstrated several TgMTPs were markedly upregulated under Cd exposure, including the TgMTP7.1. Heterologous expression in yeast validated that TgMTP7.1 could ameliorate Cd sensitivity and enhance its tolerance. These results provide primary insights into the MTP gene family in tulip. Phylogenetic relationships and functional analyses establish a framework for elucidating the transporters and molecular mechanisms governing Cd accumulation and distribution in tulip. Key TgMTPs identified, exemplified by TgMTP7.1, may illuminate molecular breeding efforts aimed at developing Cd-tolerant cultivars for the remediation of soil Cd contamination.https://www.frontiersin.org/articles/10.3389/fpls.2024.1346255/fullTulipa gesnerianaheavy metal accumulationmetal tolerance proteins (MTPs)phylogenetic analysisgene expression regulation |
| spellingShingle | Jiaojiao Lu Jiaojiao Lu Guimei Xing Guimei Xing Yanqiu Zhang Huihua Zhang Tianyu Wu Zengzhi Tian Lianwei Qu Lianwei Qu Genome-wide identification, expression and function analysis of the MTP gene family in tulip (Tulipa gesneriana) Frontiers in Plant Science Tulipa gesneriana heavy metal accumulation metal tolerance proteins (MTPs) phylogenetic analysis gene expression regulation |
| title | Genome-wide identification, expression and function analysis of the MTP gene family in tulip (Tulipa gesneriana) |
| title_full | Genome-wide identification, expression and function analysis of the MTP gene family in tulip (Tulipa gesneriana) |
| title_fullStr | Genome-wide identification, expression and function analysis of the MTP gene family in tulip (Tulipa gesneriana) |
| title_full_unstemmed | Genome-wide identification, expression and function analysis of the MTP gene family in tulip (Tulipa gesneriana) |
| title_short | Genome-wide identification, expression and function analysis of the MTP gene family in tulip (Tulipa gesneriana) |
| title_sort | genome wide identification expression and function analysis of the mtp gene family in tulip tulipa gesneriana |
| topic | Tulipa gesneriana heavy metal accumulation metal tolerance proteins (MTPs) phylogenetic analysis gene expression regulation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1346255/full |
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