Glioma cancer stem cells modulating the local tumor immune environment
Glioma stem cells (GSCs) drive the resistance mechanism in glioma tumors and mediate the suppression of innate and adaptive immune responses. Here we investigate the expression of mesenchymal-epithelial transition factor (c-Met) and Fas receptor in GSCs and their role in potentiating the tumor-media...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2022.1029657/full |
| _version_ | 1797995722567581696 |
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| author | Imran Khan Sadaf Mahfooz Busra Karacam Elif Burce Elbasan Kerime Akdur Hasiba Karimi Ayten Sakarcan Mustafa Aziz Hatiboglu Mustafa Aziz Hatiboglu |
| author_facet | Imran Khan Sadaf Mahfooz Busra Karacam Elif Burce Elbasan Kerime Akdur Hasiba Karimi Ayten Sakarcan Mustafa Aziz Hatiboglu Mustafa Aziz Hatiboglu |
| author_sort | Imran Khan |
| collection | DOAJ |
| description | Glioma stem cells (GSCs) drive the resistance mechanism in glioma tumors and mediate the suppression of innate and adaptive immune responses. Here we investigate the expression of mesenchymal-epithelial transition factor (c-Met) and Fas receptor in GSCs and their role in potentiating the tumor-mediated immune suppression through modulation of tumor infiltrating lymphocyte (TIL) population. Tumor tissues were collected from 4 patients who underwent surgery for glioblastoma. GSCs were cultured as neurospheres and evaluated for the co-expression of CD133, c-Met and FasL through flow cytometry. TILs were isolated and evaluated for the lymphocyte subset frequencies including CD3 +, CD4 +, CD8 +, regulatory T cells (FOXP3 + CD25) and microglia (CD11b + CD45) using flow cytometry. Our findings revealed that a significant population of GSCs in all four samples expressed c-Met (89–99%) and FasL (73–97%). A significantly low microglia population was found in local immune cells ranging from 3 to 5%. We did not find a statistically significant correlation between expressions of c-Met + GSC and FasL + GSC with local and systemic immune cells. This may be regarded to the small sample size. The percent c-Met + and FasL + GSC population appeared to be related to percent cytotoxic T cells, regulatory T cells and microglia populations in glioblastoma patients. Further investigation is warranted in a larger sample size. |
| first_indexed | 2024-04-11T10:06:08Z |
| format | Article |
| id | doaj.art-4868ad5671b940e1b761d7e357501447 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-04-11T10:06:08Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-4868ad5671b940e1b761d7e3575014472022-12-22T04:30:14ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992022-10-011510.3389/fnmol.2022.10296571029657Glioma cancer stem cells modulating the local tumor immune environmentImran Khan0Sadaf Mahfooz1Busra Karacam2Elif Burce Elbasan3Kerime Akdur4Hasiba Karimi5Ayten Sakarcan6Mustafa Aziz Hatiboglu7Mustafa Aziz Hatiboglu8Department of Molecular Biology, Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakif University, Istanbul, TurkeyDepartment of Molecular Biology, Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakif University, Istanbul, TurkeyDepartment of Molecular Biology, Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakif University, Istanbul, TurkeyDepartment of Neurosurgery, Bezmialem Vakif University Medical School, Istanbul, TurkeyDepartment of Neurosurgery, Bezmialem Vakif University Medical School, Istanbul, TurkeyBezmialem Vakif University Medical School, Istanbul, TurkeyDepartment of Neurosurgery, Bezmialem Vakif University Medical School, Istanbul, TurkeyDepartment of Molecular Biology, Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakif University, Istanbul, TurkeyDepartment of Neurosurgery, Bezmialem Vakif University Medical School, Istanbul, TurkeyGlioma stem cells (GSCs) drive the resistance mechanism in glioma tumors and mediate the suppression of innate and adaptive immune responses. Here we investigate the expression of mesenchymal-epithelial transition factor (c-Met) and Fas receptor in GSCs and their role in potentiating the tumor-mediated immune suppression through modulation of tumor infiltrating lymphocyte (TIL) population. Tumor tissues were collected from 4 patients who underwent surgery for glioblastoma. GSCs were cultured as neurospheres and evaluated for the co-expression of CD133, c-Met and FasL through flow cytometry. TILs were isolated and evaluated for the lymphocyte subset frequencies including CD3 +, CD4 +, CD8 +, regulatory T cells (FOXP3 + CD25) and microglia (CD11b + CD45) using flow cytometry. Our findings revealed that a significant population of GSCs in all four samples expressed c-Met (89–99%) and FasL (73–97%). A significantly low microglia population was found in local immune cells ranging from 3 to 5%. We did not find a statistically significant correlation between expressions of c-Met + GSC and FasL + GSC with local and systemic immune cells. This may be regarded to the small sample size. The percent c-Met + and FasL + GSC population appeared to be related to percent cytotoxic T cells, regulatory T cells and microglia populations in glioblastoma patients. Further investigation is warranted in a larger sample size.https://www.frontiersin.org/articles/10.3389/fnmol.2022.1029657/fullglioma stem cellsglioblastomatumor immune responsesystemic immune responsetumor microenvironment |
| spellingShingle | Imran Khan Sadaf Mahfooz Busra Karacam Elif Burce Elbasan Kerime Akdur Hasiba Karimi Ayten Sakarcan Mustafa Aziz Hatiboglu Mustafa Aziz Hatiboglu Glioma cancer stem cells modulating the local tumor immune environment Frontiers in Molecular Neuroscience glioma stem cells glioblastoma tumor immune response systemic immune response tumor microenvironment |
| title | Glioma cancer stem cells modulating the local tumor immune environment |
| title_full | Glioma cancer stem cells modulating the local tumor immune environment |
| title_fullStr | Glioma cancer stem cells modulating the local tumor immune environment |
| title_full_unstemmed | Glioma cancer stem cells modulating the local tumor immune environment |
| title_short | Glioma cancer stem cells modulating the local tumor immune environment |
| title_sort | glioma cancer stem cells modulating the local tumor immune environment |
| topic | glioma stem cells glioblastoma tumor immune response systemic immune response tumor microenvironment |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2022.1029657/full |
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