EGFR mutation status yield from bronchoalveolar lavage in patients with primary pulmonary adenocarcinoma compared to a venous blood sample and tissue biopsy
Background In recent years, there has been a revolution in the genomic profiling and molecular typing of lung cancer. A key oncogene is the epidermal growth factor receptor (EGFR). The gold standard for determining EGFR mutation status is tissue biopsy, where a histological specimen is taken by a br...
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PeerJ Inc.
2021-05-01
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| Online Access: | https://peerj.com/articles/11448.pdf |
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| author | Nikolay Yanev Evgeni Mekov Dinko Valev Georgi Yankov Vladimir Milanov Stoyan Bichev Natalia Gabrovska Dimitar Kostadinov |
| author_facet | Nikolay Yanev Evgeni Mekov Dinko Valev Georgi Yankov Vladimir Milanov Stoyan Bichev Natalia Gabrovska Dimitar Kostadinov |
| author_sort | Nikolay Yanev |
| collection | DOAJ |
| description | Background In recent years, there has been a revolution in the genomic profiling and molecular typing of lung cancer. A key oncogene is the epidermal growth factor receptor (EGFR). The gold standard for determining EGFR mutation status is tissue biopsy, where a histological specimen is taken by a bronchoscopic or surgical method (transbronchial biopsy, forceps biopsy, etc.). However, in clinical practice the tissue sample is often insufficient for morphological and molecular analysis. Bronchoalveolar lavage is a validated diagnostic method for pathogenic infections in the lower respiratory tract, yet its diagnostic value for oncogenic mutation testing in lung cancer has not been extensively investigated. This study aims to compare the prevalence of EGFR mutation status in bronchoalveolar lavage and peripheral blood referring to the gold standard - tissue biopsy in patients with primary lung adenocarcinoma. Methods Twenty-six patients with adenocarcinoma were examined for EGFR mutation from tissue biopsy, peripheral blood sample and bronchoalveolar lavage. Results Thirteen patients had wild type EGFR and the other 13 had EGFR mutation. EGFR mutation from a peripheral blood sample was identified in 38.5% (5/13) of patients, whereas EGFR mutation obtained from bronchoalveolar lavage (BAL) was identified in 92.3% (12/13). This study demonstrates that a liquid biopsy sample for EGFR status from BAL has a higher sensitivity compared to a venous blood sample. |
| first_indexed | 2024-03-09T07:22:44Z |
| format | Article |
| id | doaj.art-486a2dc44b954ff39721fda9c2d0ceb2 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:22:44Z |
| publishDate | 2021-05-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
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| spelling | doaj.art-486a2dc44b954ff39721fda9c2d0ceb22023-12-03T07:13:22ZengPeerJ Inc.PeerJ2167-83592021-05-019e1144810.7717/peerj.11448EGFR mutation status yield from bronchoalveolar lavage in patients with primary pulmonary adenocarcinoma compared to a venous blood sample and tissue biopsyNikolay Yanev0Evgeni Mekov1Dinko Valev2Georgi Yankov3Vladimir Milanov4Stoyan Bichev5Natalia Gabrovska6Dimitar Kostadinov7Department of Pulmonary Diseases, Medical University - Sofia, Sofia, BulgariaDepartment of Pulmonary Diseases, Medical University - Sofia, Sofia, BulgariaUniversity Hospital “St. Ioan Krustitel”, Sofia, BulgariaDepartment of Pulmonary Diseases, Medical University - Sofia, Sofia, BulgariaDepartment of Pulmonary Diseases, Medical University - Sofia, Sofia, BulgariaNational Genetics Laboratory, Medical University - Sofia, Sofia, BulgariaDepartment of Pulmonary Diseases, Medical University - Sofia, Sofia, BulgariaDepartment of Pulmonary Diseases, Medical University - Sofia, Sofia, BulgariaBackground In recent years, there has been a revolution in the genomic profiling and molecular typing of lung cancer. A key oncogene is the epidermal growth factor receptor (EGFR). The gold standard for determining EGFR mutation status is tissue biopsy, where a histological specimen is taken by a bronchoscopic or surgical method (transbronchial biopsy, forceps biopsy, etc.). However, in clinical practice the tissue sample is often insufficient for morphological and molecular analysis. Bronchoalveolar lavage is a validated diagnostic method for pathogenic infections in the lower respiratory tract, yet its diagnostic value for oncogenic mutation testing in lung cancer has not been extensively investigated. This study aims to compare the prevalence of EGFR mutation status in bronchoalveolar lavage and peripheral blood referring to the gold standard - tissue biopsy in patients with primary lung adenocarcinoma. Methods Twenty-six patients with adenocarcinoma were examined for EGFR mutation from tissue biopsy, peripheral blood sample and bronchoalveolar lavage. Results Thirteen patients had wild type EGFR and the other 13 had EGFR mutation. EGFR mutation from a peripheral blood sample was identified in 38.5% (5/13) of patients, whereas EGFR mutation obtained from bronchoalveolar lavage (BAL) was identified in 92.3% (12/13). This study demonstrates that a liquid biopsy sample for EGFR status from BAL has a higher sensitivity compared to a venous blood sample.https://peerj.com/articles/11448.pdfAdenocarcinomaLung cancerEGFRLiquid biopsyBronchoalveolar lavage |
| spellingShingle | Nikolay Yanev Evgeni Mekov Dinko Valev Georgi Yankov Vladimir Milanov Stoyan Bichev Natalia Gabrovska Dimitar Kostadinov EGFR mutation status yield from bronchoalveolar lavage in patients with primary pulmonary adenocarcinoma compared to a venous blood sample and tissue biopsy PeerJ Adenocarcinoma Lung cancer EGFR Liquid biopsy Bronchoalveolar lavage |
| title | EGFR mutation status yield from bronchoalveolar lavage in patients with primary pulmonary adenocarcinoma compared to a venous blood sample and tissue biopsy |
| title_full | EGFR mutation status yield from bronchoalveolar lavage in patients with primary pulmonary adenocarcinoma compared to a venous blood sample and tissue biopsy |
| title_fullStr | EGFR mutation status yield from bronchoalveolar lavage in patients with primary pulmonary adenocarcinoma compared to a venous blood sample and tissue biopsy |
| title_full_unstemmed | EGFR mutation status yield from bronchoalveolar lavage in patients with primary pulmonary adenocarcinoma compared to a venous blood sample and tissue biopsy |
| title_short | EGFR mutation status yield from bronchoalveolar lavage in patients with primary pulmonary adenocarcinoma compared to a venous blood sample and tissue biopsy |
| title_sort | egfr mutation status yield from bronchoalveolar lavage in patients with primary pulmonary adenocarcinoma compared to a venous blood sample and tissue biopsy |
| topic | Adenocarcinoma Lung cancer EGFR Liquid biopsy Bronchoalveolar lavage |
| url | https://peerj.com/articles/11448.pdf |
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