Effects of Mechanical Compression on Chondrogenesis of Human Synovium-Derived Mesenchymal Stem Cells in Agarose Hydrogel
Mechanical compression is a double-edged sword for cartilage remodeling, and the effect of mechanical compression on chondrogenic differentiation still remains elusive to date. Herein, we investigate the effect of mechanical dynamic compression on the chondrogenic differentiation of human synovium-d...
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.697281/full |
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| author | Yuxiang Ge Yuxiang Ge Yixuan Li Yixuan Li Zixu Wang Zixu Wang Lan Li Lan Li Huajian Teng Qing Jiang Qing Jiang |
| author_facet | Yuxiang Ge Yuxiang Ge Yixuan Li Yixuan Li Zixu Wang Zixu Wang Lan Li Lan Li Huajian Teng Qing Jiang Qing Jiang |
| author_sort | Yuxiang Ge |
| collection | DOAJ |
| description | Mechanical compression is a double-edged sword for cartilage remodeling, and the effect of mechanical compression on chondrogenic differentiation still remains elusive to date. Herein, we investigate the effect of mechanical dynamic compression on the chondrogenic differentiation of human synovium-derived mesenchymal stem cells (SMSCs). To this aim, SMSCs encapsulated in agarose hydrogels were cultured in chondrogenic-induced medium with or without dynamic compression. Dynamic compression was applied at either early time-point (day 1) or late time-point (day 21) during chondrogenic induction period. We found that dynamic compression initiated at early time-point downregulated the expression level of chondrocyte-specific markers as well as hypertrophy-specific markers compared with unloaded control. On the contrary, dynamic compression applied at late time-point not only enhanced the levels of cartilage matrix gene expression, but also suppressed the hypertrophic development of SMSCs compared with unloaded controls. Taken together, our findings suggest that dynamic mechanical compression loading not only promotes chondrogenic differentiation of SMSCs, but also plays a vital role in the maintenance of cartilage phenotype, and our findings also provide an experimental guide for stem cell-based cartilage repair and regeneration. |
| first_indexed | 2024-12-13T20:06:02Z |
| format | Article |
| id | doaj.art-486e7b080f2f449ca4214053ab06826a |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-13T20:06:02Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-486e7b080f2f449ca4214053ab06826a2022-12-21T23:33:01ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-07-01910.3389/fbioe.2021.697281697281Effects of Mechanical Compression on Chondrogenesis of Human Synovium-Derived Mesenchymal Stem Cells in Agarose HydrogelYuxiang Ge0Yuxiang Ge1Yixuan Li2Yixuan Li3Zixu Wang4Zixu Wang5Lan Li6Lan Li7Huajian Teng8Qing Jiang9Qing Jiang10State Key Laboratory of Pharmaceutical Biotechnology, Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, ChinaLaboratory for Bone and Joint Disease, Model Animal Research Center, Nanjing University, Nanjing, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, ChinaLaboratory for Bone and Joint Disease, Model Animal Research Center, Nanjing University, Nanjing, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, ChinaLaboratory for Bone and Joint Disease, Model Animal Research Center, Nanjing University, Nanjing, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, ChinaJiangsu Engineering Research Center for 3D Bioprinting, Nanjing, ChinaLaboratory for Bone and Joint Disease, Model Animal Research Center, Nanjing University, Nanjing, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, ChinaLaboratory for Bone and Joint Disease, Model Animal Research Center, Nanjing University, Nanjing, ChinaMechanical compression is a double-edged sword for cartilage remodeling, and the effect of mechanical compression on chondrogenic differentiation still remains elusive to date. Herein, we investigate the effect of mechanical dynamic compression on the chondrogenic differentiation of human synovium-derived mesenchymal stem cells (SMSCs). To this aim, SMSCs encapsulated in agarose hydrogels were cultured in chondrogenic-induced medium with or without dynamic compression. Dynamic compression was applied at either early time-point (day 1) or late time-point (day 21) during chondrogenic induction period. We found that dynamic compression initiated at early time-point downregulated the expression level of chondrocyte-specific markers as well as hypertrophy-specific markers compared with unloaded control. On the contrary, dynamic compression applied at late time-point not only enhanced the levels of cartilage matrix gene expression, but also suppressed the hypertrophic development of SMSCs compared with unloaded controls. Taken together, our findings suggest that dynamic mechanical compression loading not only promotes chondrogenic differentiation of SMSCs, but also plays a vital role in the maintenance of cartilage phenotype, and our findings also provide an experimental guide for stem cell-based cartilage repair and regeneration.https://www.frontiersin.org/articles/10.3389/fbioe.2021.697281/fullchondrocytesmechanical compressionchondrogenesiscartilage repairSMSCs |
| spellingShingle | Yuxiang Ge Yuxiang Ge Yixuan Li Yixuan Li Zixu Wang Zixu Wang Lan Li Lan Li Huajian Teng Qing Jiang Qing Jiang Effects of Mechanical Compression on Chondrogenesis of Human Synovium-Derived Mesenchymal Stem Cells in Agarose Hydrogel Frontiers in Bioengineering and Biotechnology chondrocytes mechanical compression chondrogenesis cartilage repair SMSCs |
| title | Effects of Mechanical Compression on Chondrogenesis of Human Synovium-Derived Mesenchymal Stem Cells in Agarose Hydrogel |
| title_full | Effects of Mechanical Compression on Chondrogenesis of Human Synovium-Derived Mesenchymal Stem Cells in Agarose Hydrogel |
| title_fullStr | Effects of Mechanical Compression on Chondrogenesis of Human Synovium-Derived Mesenchymal Stem Cells in Agarose Hydrogel |
| title_full_unstemmed | Effects of Mechanical Compression on Chondrogenesis of Human Synovium-Derived Mesenchymal Stem Cells in Agarose Hydrogel |
| title_short | Effects of Mechanical Compression on Chondrogenesis of Human Synovium-Derived Mesenchymal Stem Cells in Agarose Hydrogel |
| title_sort | effects of mechanical compression on chondrogenesis of human synovium derived mesenchymal stem cells in agarose hydrogel |
| topic | chondrocytes mechanical compression chondrogenesis cartilage repair SMSCs |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.697281/full |
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