LncRNA CRNDE inhibits cardiomyocytes apoptosis by YAP1 in myocardial ischaemia/reperfusion injury
Background Cardiomyocytes apoptosis is the basic pathological process of myocardial ischaemia/reperfusion (MI/R) injury, so inhibiting apoptosis of cardiomyocytes can effectively improve MI/R injury. Long non-coding RNA colorectal neoplasia differentially expressed (lncRNA CRNDE) can inhibit cell ap...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-05-01
|
| Series: | Autoimmunity |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/08916934.2021.1913580 |
| _version_ | 1797684598945087488 |
|---|---|
| author | Xiaohua Zhu Shuiqi Li Chen Huang Gongcheng Huang Jing Xu |
| author_facet | Xiaohua Zhu Shuiqi Li Chen Huang Gongcheng Huang Jing Xu |
| author_sort | Xiaohua Zhu |
| collection | DOAJ |
| description | Background Cardiomyocytes apoptosis is the basic pathological process of myocardial ischaemia/reperfusion (MI/R) injury, so inhibiting apoptosis of cardiomyocytes can effectively improve MI/R injury. Long non-coding RNA colorectal neoplasia differentially expressed (lncRNA CRNDE) can inhibit cell apoptosis, but its specific role in MI/R injury has not been studied. The aim of this study is to explore the specific effect of lncRNA CRNDE on cardiomyocytes apoptosis. Methods MI/R model in vivo and hypoxia/re-oxygenation (H/R) model in vitro were constructed. Apoptotic levels were assessed by TUNEL staining assay. QRT-PCR was used to validate lncRNA CRNDE level in myocardial tissues and HL-1 cells. The protein expressions of YAP1, Bcl-2 and cleaved caspase-3 were detected by western blot analysis. Flow cytometry was used to determine the apoptosis rate of cardiomyocytes. RIP assay was used to detect the interaction between lncRNA CRNDE and YAP1. Results The extent of cardiomyocytes apoptosis was significantly increased, and the levels of lncRNA CRNDE, YAP1 and Bcl-2 were down-regulated, while cleaved caspase-3 expression was up-regulated in MI/R mice and H/R-treated HL-1 cells. The expressions of YAP1 and Bcl-2 were decreased, while the expression of cleaved caspase-3 was increased after the knockdown of lncRNA CRNDE. Furthermore, lncRNA CRNDE could bind to YAP1 and regulated the protein level of YAP1 by ubiquitination and proteasomal degradation pathway. After transfection of Si-YAP1 in the H/R-treated HL-1 cells transfected with pc-DNA CRNDE, the protein level of Bcl-2 was decreased, while cleaved caspase-3 expression and the apoptosis rate were increased. Conclusion Our study suggested that lncRNA CRNDE could regulate YAP1 level by ubiquitination and proteasomal degradation pathway, thus inhibiting cardiomyocytes apoptosis in MI/R injury. |
| first_indexed | 2024-03-12T00:32:06Z |
| format | Article |
| id | doaj.art-4871bd199de74058ba6a39f28035f00b |
| institution | Directory Open Access Journal |
| issn | 0891-6934 1607-842X |
| language | English |
| last_indexed | 2024-03-12T00:32:06Z |
| publishDate | 2021-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Autoimmunity |
| spelling | doaj.art-4871bd199de74058ba6a39f28035f00b2023-09-15T10:12:23ZengTaylor & Francis GroupAutoimmunity0891-69341607-842X2021-05-0154420421210.1080/08916934.2021.19135801913580LncRNA CRNDE inhibits cardiomyocytes apoptosis by YAP1 in myocardial ischaemia/reperfusion injuryXiaohua Zhu0Shuiqi Li1Chen Huang2Gongcheng Huang3Jing Xu4Department of Cardiovascular Surgery, The First Affiliated Hospital of Zhengzhou UniversityDepartment of Dermatology, The First Affiliated Hospital of Zhengzhou UniversityDepartment of Cardiovascular Surgery, The First Affiliated Hospital of Zhengzhou UniversityDepartment of Cardiovascular Surgery, The First Affiliated Hospital of Zhengzhou UniversityDepartment of Cardiovascular Surgery, The First Affiliated Hospital of Zhengzhou UniversityBackground Cardiomyocytes apoptosis is the basic pathological process of myocardial ischaemia/reperfusion (MI/R) injury, so inhibiting apoptosis of cardiomyocytes can effectively improve MI/R injury. Long non-coding RNA colorectal neoplasia differentially expressed (lncRNA CRNDE) can inhibit cell apoptosis, but its specific role in MI/R injury has not been studied. The aim of this study is to explore the specific effect of lncRNA CRNDE on cardiomyocytes apoptosis. Methods MI/R model in vivo and hypoxia/re-oxygenation (H/R) model in vitro were constructed. Apoptotic levels were assessed by TUNEL staining assay. QRT-PCR was used to validate lncRNA CRNDE level in myocardial tissues and HL-1 cells. The protein expressions of YAP1, Bcl-2 and cleaved caspase-3 were detected by western blot analysis. Flow cytometry was used to determine the apoptosis rate of cardiomyocytes. RIP assay was used to detect the interaction between lncRNA CRNDE and YAP1. Results The extent of cardiomyocytes apoptosis was significantly increased, and the levels of lncRNA CRNDE, YAP1 and Bcl-2 were down-regulated, while cleaved caspase-3 expression was up-regulated in MI/R mice and H/R-treated HL-1 cells. The expressions of YAP1 and Bcl-2 were decreased, while the expression of cleaved caspase-3 was increased after the knockdown of lncRNA CRNDE. Furthermore, lncRNA CRNDE could bind to YAP1 and regulated the protein level of YAP1 by ubiquitination and proteasomal degradation pathway. After transfection of Si-YAP1 in the H/R-treated HL-1 cells transfected with pc-DNA CRNDE, the protein level of Bcl-2 was decreased, while cleaved caspase-3 expression and the apoptosis rate were increased. Conclusion Our study suggested that lncRNA CRNDE could regulate YAP1 level by ubiquitination and proteasomal degradation pathway, thus inhibiting cardiomyocytes apoptosis in MI/R injury.http://dx.doi.org/10.1080/08916934.2021.1913580myocardial ischaemia/reperfusion injurylncrna crndeyap1cardiomyocytes apoptosis |
| spellingShingle | Xiaohua Zhu Shuiqi Li Chen Huang Gongcheng Huang Jing Xu LncRNA CRNDE inhibits cardiomyocytes apoptosis by YAP1 in myocardial ischaemia/reperfusion injury Autoimmunity myocardial ischaemia/reperfusion injury lncrna crnde yap1 cardiomyocytes apoptosis |
| title | LncRNA CRNDE inhibits cardiomyocytes apoptosis by YAP1 in myocardial ischaemia/reperfusion injury |
| title_full | LncRNA CRNDE inhibits cardiomyocytes apoptosis by YAP1 in myocardial ischaemia/reperfusion injury |
| title_fullStr | LncRNA CRNDE inhibits cardiomyocytes apoptosis by YAP1 in myocardial ischaemia/reperfusion injury |
| title_full_unstemmed | LncRNA CRNDE inhibits cardiomyocytes apoptosis by YAP1 in myocardial ischaemia/reperfusion injury |
| title_short | LncRNA CRNDE inhibits cardiomyocytes apoptosis by YAP1 in myocardial ischaemia/reperfusion injury |
| title_sort | lncrna crnde inhibits cardiomyocytes apoptosis by yap1 in myocardial ischaemia reperfusion injury |
| topic | myocardial ischaemia/reperfusion injury lncrna crnde yap1 cardiomyocytes apoptosis |
| url | http://dx.doi.org/10.1080/08916934.2021.1913580 |
| work_keys_str_mv | AT xiaohuazhu lncrnacrndeinhibitscardiomyocytesapoptosisbyyap1inmyocardialischaemiareperfusioninjury AT shuiqili lncrnacrndeinhibitscardiomyocytesapoptosisbyyap1inmyocardialischaemiareperfusioninjury AT chenhuang lncrnacrndeinhibitscardiomyocytesapoptosisbyyap1inmyocardialischaemiareperfusioninjury AT gongchenghuang lncrnacrndeinhibitscardiomyocytesapoptosisbyyap1inmyocardialischaemiareperfusioninjury AT jingxu lncrnacrndeinhibitscardiomyocytesapoptosisbyyap1inmyocardialischaemiareperfusioninjury |