A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode
In this study, a homogeneous method featuring simple, one-step detection was developed to analyze hippuric acid (HA), a major metabolite of toluene. High sensitivity was achieved with the facile immobilization of poly(vinylimidazole) (PVI) on an indium tin oxide (ITO) electrode. Using a previously d...
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| Format: | Article |
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MDPI AG
2016-12-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/17/1/54 |
| _version_ | 1828351164478914560 |
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| author | Young-Bong Choi Won-Yong Jeon Hyug-Han Kim |
| author_facet | Young-Bong Choi Won-Yong Jeon Hyug-Han Kim |
| author_sort | Young-Bong Choi |
| collection | DOAJ |
| description | In this study, a homogeneous method featuring simple, one-step detection was developed to analyze hippuric acid (HA), a major metabolite of toluene. High sensitivity was achieved with the facile immobilization of poly(vinylimidazole) (PVI) on an indium tin oxide (ITO) electrode. Using a previously developed approach, pentacyanoferrate was coordinated with pyridyl-N ligands, and the redox-active Fe(II/III) centers were bound to Ni(II) ions on the electrode via electrostatic cyanide bridges. The detection was accomplished by the competitive binding of free HA and pentacyanoferrate-(4-aminomethylpyridine-hippuric acid) (Fe-HA, the electron transfer mediator) to the HA antibody on the Ni(II) ions-modified PVI-ITO (Ni-PVI-ITO) electrode. The electrical and physicochemical characterization of the electrode was carried out by cyclic voltammetry, differential pulse voltammetry, field emission scanning electron microscopy, and X-ray photoelectron spectroscopy. At low mediator concentrations, the electrical signals were proportional to the HA concentration between 0.1 µg/mL and 1.0 mg/mL. The same method may be extended to other small organic molecules. |
| first_indexed | 2024-04-14T01:35:35Z |
| format | Article |
| id | doaj.art-48749a8a8f28471480f9a90b7693880c |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T01:35:35Z |
| publishDate | 2016-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-48749a8a8f28471480f9a90b7693880c2022-12-22T02:19:56ZengMDPI AGSensors1424-82202016-12-011715410.3390/s17010054s17010054A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified ElectrodeYoung-Bong Choi0Won-Yong Jeon1Hyug-Han Kim2Department of Chemistry, College of Natural Science, Dankook University, Anseo-Dong, Cheonan, Chungnam 330-714, KoreaDepartment of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Anseo-Dong, Cheonan, Chungnam 330-714, KoreaDepartment of Chemistry, College of Natural Science, Dankook University, Anseo-Dong, Cheonan, Chungnam 330-714, KoreaIn this study, a homogeneous method featuring simple, one-step detection was developed to analyze hippuric acid (HA), a major metabolite of toluene. High sensitivity was achieved with the facile immobilization of poly(vinylimidazole) (PVI) on an indium tin oxide (ITO) electrode. Using a previously developed approach, pentacyanoferrate was coordinated with pyridyl-N ligands, and the redox-active Fe(II/III) centers were bound to Ni(II) ions on the electrode via electrostatic cyanide bridges. The detection was accomplished by the competitive binding of free HA and pentacyanoferrate-(4-aminomethylpyridine-hippuric acid) (Fe-HA, the electron transfer mediator) to the HA antibody on the Ni(II) ions-modified PVI-ITO (Ni-PVI-ITO) electrode. The electrical and physicochemical characterization of the electrode was carried out by cyclic voltammetry, differential pulse voltammetry, field emission scanning electron microscopy, and X-ray photoelectron spectroscopy. At low mediator concentrations, the electrical signals were proportional to the HA concentration between 0.1 µg/mL and 1.0 mg/mL. The same method may be extended to other small organic molecules.http://www.mdpi.com/1424-8220/17/1/54immunoassayhippuric acidelectrochemical biosensornickel ionmediator |
| spellingShingle | Young-Bong Choi Won-Yong Jeon Hyug-Han Kim A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode Sensors immunoassay hippuric acid electrochemical biosensor nickel ion mediator |
| title | A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode |
| title_full | A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode |
| title_fullStr | A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode |
| title_full_unstemmed | A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode |
| title_short | A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode |
| title_sort | simple interfacial platform for homogeneous electrochemical immunoassays using a poly vinylimidazole modified electrode |
| topic | immunoassay hippuric acid electrochemical biosensor nickel ion mediator |
| url | http://www.mdpi.com/1424-8220/17/1/54 |
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