The Effects of Acute Bisphenol A Toxicity on the Hematological Parameters, Hematopoiesis, and Kidney Histology of Zebrafish (<i>Danio rerio</i>)
In this study, the results of evaluating the acute toxicity of Bisphenol A on <i>Danio rerio</i> are presented, encompassing peripheral blood parameters, the composition of hematopoietic cells of erythroid and myeloid lines in the head kidney, and data from histological studies. The LC50...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/23/3685 |
| _version_ | 1797400513326612480 |
|---|---|
| author | Svetlana Smorodinskaya Nikita Kochetkov Kirill Gavrilin Dmitry Nikiforov-Nikishin Diana Reznikova Aleksey Vatlin Anastasia Klimuk Maya Odorskaya Alexei Nikiforov-Nikishin Andrey Ponomarev Maria Marsova Valery Danilenko |
| author_facet | Svetlana Smorodinskaya Nikita Kochetkov Kirill Gavrilin Dmitry Nikiforov-Nikishin Diana Reznikova Aleksey Vatlin Anastasia Klimuk Maya Odorskaya Alexei Nikiforov-Nikishin Andrey Ponomarev Maria Marsova Valery Danilenko |
| author_sort | Svetlana Smorodinskaya |
| collection | DOAJ |
| description | In this study, the results of evaluating the acute toxicity of Bisphenol A on <i>Danio rerio</i> are presented, encompassing peripheral blood parameters, the composition of hematopoietic cells of erythroid and myeloid lines in the head kidney, and data from histological studies. The LC50 values of Bisphenol A for adult zebrafish individuals for 12, 24, and 48–96 h were determined, which were 18.04, 7.55, and 6.22 mg/L, respectively. The study includes data on the morphology and quantitative frequency of specific cells in the hematopoietic tissue of the head kidney, along with the consideration of adaptive mechanisms in hematopoiesis under BPA exposure. The application of polynomial regression analysis to reveal the concentration–effect relationship for some hematological and histological parameters was demonstrated. Significant increases in the frequency of erythrocyte nuclear abnormalities were observed at BPA concentrations of 6 and 8 mg/L, which indicates a genotoxic effect. BPA’s impact on fish peripheral blood parameters manifested as an increase in the number of erythrocytes (RBC) and immature erythrocytes, as well as a decrease in the number of lymphocytes. The most notable pathological changes in the head kidney’s hematopoietic tissue included circulatory disturbances and the formation of inflammation/degradation foci, as confirmed by histopathologic indices. At BPA concentrations of 2 and 4 mg/L, the observed changes were compensated for by hematopoietic adaptation mechanisms; however, at concentrations of 6 and 8 mg/L, acute systemic toxicity was evident. |
| first_indexed | 2024-03-09T01:55:36Z |
| format | Article |
| id | doaj.art-48757095750d4159a497805e395ac864 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T01:55:36Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-48757095750d4159a497805e395ac8642023-12-08T15:10:41ZengMDPI AGAnimals2076-26152023-11-011323368510.3390/ani13233685The Effects of Acute Bisphenol A Toxicity on the Hematological Parameters, Hematopoiesis, and Kidney Histology of Zebrafish (<i>Danio rerio</i>)Svetlana Smorodinskaya0Nikita Kochetkov1Kirill Gavrilin2Dmitry Nikiforov-Nikishin3Diana Reznikova4Aleksey Vatlin5Anastasia Klimuk6Maya Odorskaya7Alexei Nikiforov-Nikishin8Andrey Ponomarev9Maria Marsova10Valery Danilenko11Laboratory of Bacterial Genetics, Vavilov Institute of General Genetics Russian Academy of Sciences, 119333 Moscow, RussiaLaboratory of Bacterial Genetics, Vavilov Institute of General Genetics Russian Academy of Sciences, 119333 Moscow, RussiaFaculty of Biotechnology and Fisheries, Moscow State University of Technologies and Management (FCU), 73, Zemlyanoy Val Str., 109004 Moscow, RussiaLaboratory of Bacterial Genetics, Vavilov Institute of General Genetics Russian Academy of Sciences, 119333 Moscow, RussiaLaboratory of Bacterial Genetics, Vavilov Institute of General Genetics Russian Academy of Sciences, 119333 Moscow, RussiaLaboratory of Bacterial Genetics, Vavilov Institute of General Genetics Russian Academy of Sciences, 119333 Moscow, RussiaLaboratory of Bacterial Genetics, Vavilov Institute of General Genetics Russian Academy of Sciences, 119333 Moscow, RussiaLaboratory of Bacterial Genetics, Vavilov Institute of General Genetics Russian Academy of Sciences, 119333 Moscow, RussiaFaculty of Biotechnology and Fisheries, Moscow State University of Technologies and Management (FCU), 73, Zemlyanoy Val Str., 109004 Moscow, RussiaFaculty of Biotechnology and Fisheries, Moscow State University of Technologies and Management (FCU), 73, Zemlyanoy Val Str., 109004 Moscow, RussiaLaboratory of Bacterial Genetics, Vavilov Institute of General Genetics Russian Academy of Sciences, 119333 Moscow, RussiaLaboratory of Bacterial Genetics, Vavilov Institute of General Genetics Russian Academy of Sciences, 119333 Moscow, RussiaIn this study, the results of evaluating the acute toxicity of Bisphenol A on <i>Danio rerio</i> are presented, encompassing peripheral blood parameters, the composition of hematopoietic cells of erythroid and myeloid lines in the head kidney, and data from histological studies. The LC50 values of Bisphenol A for adult zebrafish individuals for 12, 24, and 48–96 h were determined, which were 18.04, 7.55, and 6.22 mg/L, respectively. The study includes data on the morphology and quantitative frequency of specific cells in the hematopoietic tissue of the head kidney, along with the consideration of adaptive mechanisms in hematopoiesis under BPA exposure. The application of polynomial regression analysis to reveal the concentration–effect relationship for some hematological and histological parameters was demonstrated. Significant increases in the frequency of erythrocyte nuclear abnormalities were observed at BPA concentrations of 6 and 8 mg/L, which indicates a genotoxic effect. BPA’s impact on fish peripheral blood parameters manifested as an increase in the number of erythrocytes (RBC) and immature erythrocytes, as well as a decrease in the number of lymphocytes. The most notable pathological changes in the head kidney’s hematopoietic tissue included circulatory disturbances and the formation of inflammation/degradation foci, as confirmed by histopathologic indices. At BPA concentrations of 2 and 4 mg/L, the observed changes were compensated for by hematopoietic adaptation mechanisms; however, at concentrations of 6 and 8 mg/L, acute systemic toxicity was evident.https://www.mdpi.com/2076-2615/13/23/3685LC50hematopoiesishead kidneyhistologygenotoxicityBisphenol A |
| spellingShingle | Svetlana Smorodinskaya Nikita Kochetkov Kirill Gavrilin Dmitry Nikiforov-Nikishin Diana Reznikova Aleksey Vatlin Anastasia Klimuk Maya Odorskaya Alexei Nikiforov-Nikishin Andrey Ponomarev Maria Marsova Valery Danilenko The Effects of Acute Bisphenol A Toxicity on the Hematological Parameters, Hematopoiesis, and Kidney Histology of Zebrafish (<i>Danio rerio</i>) Animals LC50 hematopoiesis head kidney histology genotoxicity Bisphenol A |
| title | The Effects of Acute Bisphenol A Toxicity on the Hematological Parameters, Hematopoiesis, and Kidney Histology of Zebrafish (<i>Danio rerio</i>) |
| title_full | The Effects of Acute Bisphenol A Toxicity on the Hematological Parameters, Hematopoiesis, and Kidney Histology of Zebrafish (<i>Danio rerio</i>) |
| title_fullStr | The Effects of Acute Bisphenol A Toxicity on the Hematological Parameters, Hematopoiesis, and Kidney Histology of Zebrafish (<i>Danio rerio</i>) |
| title_full_unstemmed | The Effects of Acute Bisphenol A Toxicity on the Hematological Parameters, Hematopoiesis, and Kidney Histology of Zebrafish (<i>Danio rerio</i>) |
| title_short | The Effects of Acute Bisphenol A Toxicity on the Hematological Parameters, Hematopoiesis, and Kidney Histology of Zebrafish (<i>Danio rerio</i>) |
| title_sort | effects of acute bisphenol a toxicity on the hematological parameters hematopoiesis and kidney histology of zebrafish i danio rerio i |
| topic | LC50 hematopoiesis head kidney histology genotoxicity Bisphenol A |
| url | https://www.mdpi.com/2076-2615/13/23/3685 |
| work_keys_str_mv | AT svetlanasmorodinskaya theeffectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT nikitakochetkov theeffectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT kirillgavrilin theeffectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT dmitrynikiforovnikishin theeffectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT dianareznikova theeffectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT alekseyvatlin theeffectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT anastasiaklimuk theeffectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT mayaodorskaya theeffectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT alexeinikiforovnikishin theeffectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT andreyponomarev theeffectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT mariamarsova theeffectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT valerydanilenko theeffectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT svetlanasmorodinskaya effectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT nikitakochetkov effectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT kirillgavrilin effectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT dmitrynikiforovnikishin effectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT dianareznikova effectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT alekseyvatlin effectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT anastasiaklimuk effectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT mayaodorskaya effectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT alexeinikiforovnikishin effectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT andreyponomarev effectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT mariamarsova effectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi AT valerydanilenko effectsofacutebisphenolatoxicityonthehematologicalparametershematopoiesisandkidneyhistologyofzebrafishidaniorerioi |