Enrichment of human embryonic stem cell-derived V3 interneurons using an Nkx2-2 gene-specific reporter
Abstract V3 spinal interneurons are a key element of the spinal circuits, which control motor function. However, to date, there are no effective ways of deriving a pure V3 population from human pluripotent stem cells. Here, we report a method for differentiation and isolation of spinal V3 interneuro...
Main Authors: | Ieva Berzanskyte, Federica Riccio, Carolina Barcellos Machado, Elizabeth J. Bradbury, Ivo Lieberam |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2023-02-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-023-29165-z |
Similar Items
-
The requirement of Nkx2-1 in the temporal specification of cortical interneuron subtypes.
by: Butt, S, et al.
Published: (2008) -
Sleep down state-active ID2/Nkx2.1 interneurons in the neocortex
by: Valero, M, et al.
Published: (2021) -
Thyroid Progenitors Are Robustly Derived from Embryonic Stem Cells through Transient, Developmental Stage-Specific Overexpression of Nkx2-1
by: Keri Dame, et al.
Published: (2017-02-01) -
Marking embryonic stem cells with a 2A self-cleaving peptide: a NKX2-5 emerald GFP BAC reporter.
by: Edward C Hsiao, et al.
Published: (2008-07-01) -
Esketamine induces embryonic and cardiac malformation through regulating the nkx2.5 and gata4 in zebrafish
by: Shuang Li, et al.
Published: (2025-02-01)