Diterpenoids as potential anti-malarial compounds from Andrographis paniculata
Abstract Background The objectives of the current study are to evaluate the traditionally used medicinal plants Andrographis paniculata for in vitro anti-malarial activity against human malarial parasite Plasmodium falciparum and to further characterize the anti-malarial active extract of A. panicul...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-01-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43088-021-00098-8 |
| _version_ | 1818330801155080192 |
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| author | Manish Kumar Dwivedi Shringika Mishra Shruti Sonter Prashant Kumar Singh |
| author_facet | Manish Kumar Dwivedi Shringika Mishra Shruti Sonter Prashant Kumar Singh |
| author_sort | Manish Kumar Dwivedi |
| collection | DOAJ |
| description | Abstract Background The objectives of the current study are to evaluate the traditionally used medicinal plants Andrographis paniculata for in vitro anti-malarial activity against human malarial parasite Plasmodium falciparum and to further characterize the anti-malarial active extract of A. paniculata using spectroscopic and chromatographic methods. Results The chloroform extract of A. paniculata displayed anti-malarial activity with IC50 values 6.36 μg/ml against 3D7 strain and 5.24 μg/ml against K1 strains respectively with no evidence of significant cytotoxicity against mammalian cell line (CC50 > 100 μg/ml). LC-MS analysis of the extract led to the identification of 59 compounds based on their chromatographic and mass spectrometric features (a total of 35 compounds are present in positive ion and 24 compounds in negative ion mode). We have identified 5 flavonoids and 30 compounds as diterpenoids in positive ion mode, while in the negative mode all identified compounds were diterpenoids. Characterization of the most promising class of compound diterpenoids using HPLC-LC-ESI-MS/MS was also undertaken. Conclusions The in vitro results undoubtedly validate the traditional use of A. paniculata for the treatment of malaria. The results have led to the identification of diterpenoids from IGNTU_06 extract as potential anti-malarial compounds that need to be further purified and analyzed in anti-malarial drug development programs. Graphical abstract |
| first_indexed | 2024-12-13T13:09:43Z |
| format | Article |
| id | doaj.art-4877f916b82043c7ad416f25694bf0a3 |
| institution | Directory Open Access Journal |
| issn | 2314-8543 |
| language | English |
| last_indexed | 2024-12-13T13:09:43Z |
| publishDate | 2021-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-4877f916b82043c7ad416f25694bf0a32022-12-21T23:44:43ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432021-01-0110111610.1186/s43088-021-00098-8Diterpenoids as potential anti-malarial compounds from Andrographis paniculataManish Kumar Dwivedi0Shringika Mishra1Shruti Sonter2Prashant Kumar Singh3Department of Biotechnology, Indira Gandhi National Tribal UniversityDepartment of Biotechnology, Indira Gandhi National Tribal UniversityDepartment of Biotechnology, Indira Gandhi National Tribal UniversityDepartment of Biotechnology, Indira Gandhi National Tribal UniversityAbstract Background The objectives of the current study are to evaluate the traditionally used medicinal plants Andrographis paniculata for in vitro anti-malarial activity against human malarial parasite Plasmodium falciparum and to further characterize the anti-malarial active extract of A. paniculata using spectroscopic and chromatographic methods. Results The chloroform extract of A. paniculata displayed anti-malarial activity with IC50 values 6.36 μg/ml against 3D7 strain and 5.24 μg/ml against K1 strains respectively with no evidence of significant cytotoxicity against mammalian cell line (CC50 > 100 μg/ml). LC-MS analysis of the extract led to the identification of 59 compounds based on their chromatographic and mass spectrometric features (a total of 35 compounds are present in positive ion and 24 compounds in negative ion mode). We have identified 5 flavonoids and 30 compounds as diterpenoids in positive ion mode, while in the negative mode all identified compounds were diterpenoids. Characterization of the most promising class of compound diterpenoids using HPLC-LC-ESI-MS/MS was also undertaken. Conclusions The in vitro results undoubtedly validate the traditional use of A. paniculata for the treatment of malaria. The results have led to the identification of diterpenoids from IGNTU_06 extract as potential anti-malarial compounds that need to be further purified and analyzed in anti-malarial drug development programs. Graphical abstracthttps://doi.org/10.1186/s43088-021-00098-8Andrographis panicualtaDiterpenoidIn vitro anti-malarial activityLiquid chromatographyTraditional medicine |
| spellingShingle | Manish Kumar Dwivedi Shringika Mishra Shruti Sonter Prashant Kumar Singh Diterpenoids as potential anti-malarial compounds from Andrographis paniculata Beni-Suef University Journal of Basic and Applied Sciences Andrographis panicualta Diterpenoid In vitro anti-malarial activity Liquid chromatography Traditional medicine |
| title | Diterpenoids as potential anti-malarial compounds from Andrographis paniculata |
| title_full | Diterpenoids as potential anti-malarial compounds from Andrographis paniculata |
| title_fullStr | Diterpenoids as potential anti-malarial compounds from Andrographis paniculata |
| title_full_unstemmed | Diterpenoids as potential anti-malarial compounds from Andrographis paniculata |
| title_short | Diterpenoids as potential anti-malarial compounds from Andrographis paniculata |
| title_sort | diterpenoids as potential anti malarial compounds from andrographis paniculata |
| topic | Andrographis panicualta Diterpenoid In vitro anti-malarial activity Liquid chromatography Traditional medicine |
| url | https://doi.org/10.1186/s43088-021-00098-8 |
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