Protocol for measuring sphingolipid metabolism in budding yeast
Summary: Sphingolipid biosynthesis occurs in both the endoplasmic reticulum (ER) and the Golgi apparatus. Ceramide synthesized in the ER is transported to the Golgi and incorporated into complex sphingolipids. Here, we present a step-by-step protocol to analyze sphingolipid metabolism in budding yea...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721001192 |
| _version_ | 1818669029694373888 |
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| author | Atsuko Ikeda Kazuki Hanaoka Kouichi Funato |
| author_facet | Atsuko Ikeda Kazuki Hanaoka Kouichi Funato |
| author_sort | Atsuko Ikeda |
| collection | DOAJ |
| description | Summary: Sphingolipid biosynthesis occurs in both the endoplasmic reticulum (ER) and the Golgi apparatus. Ceramide synthesized in the ER is transported to the Golgi and incorporated into complex sphingolipids. Here, we present a step-by-step protocol to analyze sphingolipid metabolism in budding yeast. Ceramide and inositolphosphorylceramide (IPC) are classes of sphingolipids present in yeast and are metabolically labeled with radioactive precursors. This protocol for metabolic labeling can be used to investigate ceramide transport in an in vivo environment.For complete details on the use and execution of this protocol, please refer to Ikeda et al. (2020). |
| first_indexed | 2024-12-17T06:45:43Z |
| format | Article |
| id | doaj.art-487b093f255b4a3487be101b676d1831 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-17T06:45:43Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-487b093f255b4a3487be101b676d18312022-12-21T21:59:44ZengElsevierSTAR Protocols2666-16672021-06-0122100412Protocol for measuring sphingolipid metabolism in budding yeastAtsuko Ikeda0Kazuki Hanaoka1Kouichi Funato2Graduate School of Biosphere Science, Hiroshima University, Kagamiyama 1-4-4, Higashi-Hiroshima 739-8528, Japan; Graduate School of Integrated Sciences for Life, Hiroshima University, Kagamiyama 1-4-4, Higashi-Hiroshima 739-8528, JapanSchool of Applied Biological Science, Hiroshima University, Kagamiyama 1-4-4, Higashi-Hiroshima 739-8528, JapanGraduate School of Biosphere Science, Hiroshima University, Kagamiyama 1-4-4, Higashi-Hiroshima 739-8528, Japan; Graduate School of Integrated Sciences for Life, Hiroshima University, Kagamiyama 1-4-4, Higashi-Hiroshima 739-8528, Japan; School of Applied Biological Science, Hiroshima University, Kagamiyama 1-4-4, Higashi-Hiroshima 739-8528, Japan; Corresponding authorSummary: Sphingolipid biosynthesis occurs in both the endoplasmic reticulum (ER) and the Golgi apparatus. Ceramide synthesized in the ER is transported to the Golgi and incorporated into complex sphingolipids. Here, we present a step-by-step protocol to analyze sphingolipid metabolism in budding yeast. Ceramide and inositolphosphorylceramide (IPC) are classes of sphingolipids present in yeast and are metabolically labeled with radioactive precursors. This protocol for metabolic labeling can be used to investigate ceramide transport in an in vivo environment.For complete details on the use and execution of this protocol, please refer to Ikeda et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721001192Cell BiologyMetabolismModel OrganismsMolecular Biology |
| spellingShingle | Atsuko Ikeda Kazuki Hanaoka Kouichi Funato Protocol for measuring sphingolipid metabolism in budding yeast STAR Protocols Cell Biology Metabolism Model Organisms Molecular Biology |
| title | Protocol for measuring sphingolipid metabolism in budding yeast |
| title_full | Protocol for measuring sphingolipid metabolism in budding yeast |
| title_fullStr | Protocol for measuring sphingolipid metabolism in budding yeast |
| title_full_unstemmed | Protocol for measuring sphingolipid metabolism in budding yeast |
| title_short | Protocol for measuring sphingolipid metabolism in budding yeast |
| title_sort | protocol for measuring sphingolipid metabolism in budding yeast |
| topic | Cell Biology Metabolism Model Organisms Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721001192 |
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