Natural Diversity in Pentose Fermentation Is Explained by Variations in Histone Deacetylases
The extent to which carbon flux is directed toward fermentation versus respiration differs between cell types and environmental conditions. Understanding the basic cellular processes governing carbon flux is challenged by the complexity of the metabolic and regulatory networks. To reveal the genetic...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-01-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124715014898 |
| _version_ | 1811236804031938560 |
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| author | Zvi Tamari Naama Barkai |
| author_facet | Zvi Tamari Naama Barkai |
| author_sort | Zvi Tamari |
| collection | DOAJ |
| description | The extent to which carbon flux is directed toward fermentation versus respiration differs between cell types and environmental conditions. Understanding the basic cellular processes governing carbon flux is challenged by the complexity of the metabolic and regulatory networks. To reveal the genetic basis for natural diversity in channeling carbon flux, we applied quantitative trait loci analysis by phenotyping and genotyping hundreds of individual F2 segregants of budding yeast that differ in their capacity to ferment the pentose sugar xylulose. Causal alleles were mapped to the RXT3 and PHO23 genes, two components of the large Rpd3 histone deacetylation complex. We show that these allelic variants modulate the expression of SNF1/AMPK-dependent respiratory genes. Our results suggest that over close evolutionary distances, diversification of carbon flow is driven by changes in global regulators, rather than adaptation of specific metabolic nodes. Such regulators may improve the ability to direct metabolic fluxes for biotechnological applications. |
| first_indexed | 2024-04-12T12:14:22Z |
| format | Article |
| id | doaj.art-487cd5b24d784030a81c2f0989bd3aab |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-12T12:14:22Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-487cd5b24d784030a81c2f0989bd3aab2022-12-22T03:33:28ZengElsevierCell Reports2211-12472016-01-0114345846310.1016/j.celrep.2015.12.048Natural Diversity in Pentose Fermentation Is Explained by Variations in Histone DeacetylasesZvi Tamari0Naama Barkai1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, IsraelDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, IsraelThe extent to which carbon flux is directed toward fermentation versus respiration differs between cell types and environmental conditions. Understanding the basic cellular processes governing carbon flux is challenged by the complexity of the metabolic and regulatory networks. To reveal the genetic basis for natural diversity in channeling carbon flux, we applied quantitative trait loci analysis by phenotyping and genotyping hundreds of individual F2 segregants of budding yeast that differ in their capacity to ferment the pentose sugar xylulose. Causal alleles were mapped to the RXT3 and PHO23 genes, two components of the large Rpd3 histone deacetylation complex. We show that these allelic variants modulate the expression of SNF1/AMPK-dependent respiratory genes. Our results suggest that over close evolutionary distances, diversification of carbon flow is driven by changes in global regulators, rather than adaptation of specific metabolic nodes. Such regulators may improve the ability to direct metabolic fluxes for biotechnological applications.http://www.sciencedirect.com/science/article/pii/S2211124715014898 |
| spellingShingle | Zvi Tamari Naama Barkai Natural Diversity in Pentose Fermentation Is Explained by Variations in Histone Deacetylases Cell Reports |
| title | Natural Diversity in Pentose Fermentation Is Explained by Variations in Histone Deacetylases |
| title_full | Natural Diversity in Pentose Fermentation Is Explained by Variations in Histone Deacetylases |
| title_fullStr | Natural Diversity in Pentose Fermentation Is Explained by Variations in Histone Deacetylases |
| title_full_unstemmed | Natural Diversity in Pentose Fermentation Is Explained by Variations in Histone Deacetylases |
| title_short | Natural Diversity in Pentose Fermentation Is Explained by Variations in Histone Deacetylases |
| title_sort | natural diversity in pentose fermentation is explained by variations in histone deacetylases |
| url | http://www.sciencedirect.com/science/article/pii/S2211124715014898 |
| work_keys_str_mv | AT zvitamari naturaldiversityinpentosefermentationisexplainedbyvariationsinhistonedeacetylases AT naamabarkai naturaldiversityinpentosefermentationisexplainedbyvariationsinhistonedeacetylases |