Impact of Ag Nanoparticles on Seed Germination and Seedling Growth of Green Beans in Normal and Chill Temperatures
The study analysed the effect of silver nanoparticles (AgNPs) on seed germination, field emergence and the physiological parameters of seedlings of two bean cultivars, ‘Bali’ and ‘Delfina’, under normal and chill temperatures. AgNPs solutions (0.25, 1.25 and 2.5 mg dm<sup>−3</sup>) were...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Agriculture |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0472/10/8/312 |
| _version_ | 1797561216423428096 |
|---|---|
| author | Roman Prażak Agata Święciło Anna Krzepiłko Sławomir Michałek Marta Arczewska |
| author_facet | Roman Prażak Agata Święciło Anna Krzepiłko Sławomir Michałek Marta Arczewska |
| author_sort | Roman Prażak |
| collection | DOAJ |
| description | The study analysed the effect of silver nanoparticles (AgNPs) on seed germination, field emergence and the physiological parameters of seedlings of two bean cultivars, ‘Bali’ and ‘Delfina’, under normal and chill temperatures. AgNPs solutions (0.25, 1.25 and 2.5 mg dm<sup>−3</sup>) were applied together with the microbial preparation Nitragina (containing <i>Rhizobium leguminosarum</i> bv. <i>phaseoli</i>) on seeds as a short-term pre-sowing treatment. Low concentrations of AgNPs (0.25, 1.25 mg dm<sup>−3</sup>) had an immediate beneficial effect, resulting in fast and uniform germination in laboratory and field conditions, as well as a positive effect in the later stages of seedling development, manifested as an increase in the average seedling height, fresh and dry weight and net photosynthesis. Particularly, favourable effects were noted in suboptimal temperature conditions, suggesting that AgNPs activate plant mechanisms of tolerance to environmental stress. The highest concentration tested of AgNPs was not particularly effective for the plants but had a strong antimicrobial effect, which was beneficial in period of seed germination, but at the later stage of plant development was unfavourable probably due to disruption of symbiosis between the bean seedlings and rhizobia. |
| first_indexed | 2024-03-10T18:10:59Z |
| format | Article |
| id | doaj.art-48802b1932da4c289a0aa96e3873457c |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T18:10:59Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-48802b1932da4c289a0aa96e3873457c2023-11-20T08:05:49ZengMDPI AGAgriculture2077-04722020-07-0110831210.3390/agriculture10080312Impact of Ag Nanoparticles on Seed Germination and Seedling Growth of Green Beans in Normal and Chill TemperaturesRoman Prażak0Agata Święciło1Anna Krzepiłko2Sławomir Michałek3Marta Arczewska4Institute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences in Lublin, St. Akademicka 15, 20-950 Lublin, PolandDepartment of Environmental Microbiology, University of Life Sciences in Lublin, St. Leszczyńskiego 7, 20-069 Lublin, PolandFaculty of Food Sciences and Biotechnology, University of Life Sciences in Lublin, St. Skromna 8, 20-704 Lublin, PolandDepartment of Botany and Plant Physiology, Faculty of Environmental Biology, University of Life Sciences in Lublin, St. Akademicka 15, 20-950 Lublin, PolandDepartment of Biophysics, Faculty of Environmental Biology, University of Life Sciences in Lublin, 20-950 Lublin, PolandThe study analysed the effect of silver nanoparticles (AgNPs) on seed germination, field emergence and the physiological parameters of seedlings of two bean cultivars, ‘Bali’ and ‘Delfina’, under normal and chill temperatures. AgNPs solutions (0.25, 1.25 and 2.5 mg dm<sup>−3</sup>) were applied together with the microbial preparation Nitragina (containing <i>Rhizobium leguminosarum</i> bv. <i>phaseoli</i>) on seeds as a short-term pre-sowing treatment. Low concentrations of AgNPs (0.25, 1.25 mg dm<sup>−3</sup>) had an immediate beneficial effect, resulting in fast and uniform germination in laboratory and field conditions, as well as a positive effect in the later stages of seedling development, manifested as an increase in the average seedling height, fresh and dry weight and net photosynthesis. Particularly, favourable effects were noted in suboptimal temperature conditions, suggesting that AgNPs activate plant mechanisms of tolerance to environmental stress. The highest concentration tested of AgNPs was not particularly effective for the plants but had a strong antimicrobial effect, which was beneficial in period of seed germination, but at the later stage of plant development was unfavourable probably due to disruption of symbiosis between the bean seedlings and rhizobia.https://www.mdpi.com/2077-0472/10/8/312AgNPsgreen beanchilling stressroot nodulesplant growthphotosynthesis |
| spellingShingle | Roman Prażak Agata Święciło Anna Krzepiłko Sławomir Michałek Marta Arczewska Impact of Ag Nanoparticles on Seed Germination and Seedling Growth of Green Beans in Normal and Chill Temperatures Agriculture AgNPs green bean chilling stress root nodules plant growth photosynthesis |
| title | Impact of Ag Nanoparticles on Seed Germination and Seedling Growth of Green Beans in Normal and Chill Temperatures |
| title_full | Impact of Ag Nanoparticles on Seed Germination and Seedling Growth of Green Beans in Normal and Chill Temperatures |
| title_fullStr | Impact of Ag Nanoparticles on Seed Germination and Seedling Growth of Green Beans in Normal and Chill Temperatures |
| title_full_unstemmed | Impact of Ag Nanoparticles on Seed Germination and Seedling Growth of Green Beans in Normal and Chill Temperatures |
| title_short | Impact of Ag Nanoparticles on Seed Germination and Seedling Growth of Green Beans in Normal and Chill Temperatures |
| title_sort | impact of ag nanoparticles on seed germination and seedling growth of green beans in normal and chill temperatures |
| topic | AgNPs green bean chilling stress root nodules plant growth photosynthesis |
| url | https://www.mdpi.com/2077-0472/10/8/312 |
| work_keys_str_mv | AT romanprazak impactofagnanoparticlesonseedgerminationandseedlinggrowthofgreenbeansinnormalandchilltemperatures AT agataswieciło impactofagnanoparticlesonseedgerminationandseedlinggrowthofgreenbeansinnormalandchilltemperatures AT annakrzepiłko impactofagnanoparticlesonseedgerminationandseedlinggrowthofgreenbeansinnormalandchilltemperatures AT sławomirmichałek impactofagnanoparticlesonseedgerminationandseedlinggrowthofgreenbeansinnormalandchilltemperatures AT martaarczewska impactofagnanoparticlesonseedgerminationandseedlinggrowthofgreenbeansinnormalandchilltemperatures |