Nutrient Deprivation Coupled with High Light Exposure for Bioactive Chrysolaminarin Production in the Marine Microalga <i>Isochrysis zhangjiangensis</i>
Chrysolaminarin, a kind of water-soluble bioactive β-glucan produced by certain microalgae, is a potential candidate for food/pharmaceutical applications. This study identified a marine microalga <i>Isochrysis zhangjiangensis</i>, in which chrysolaminarin production was investigated via...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/6/351 |
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| author | Xiuyuan Ran Yuhan Shen Dongjian Jiang Chenqi Wang Xinghui Li Haoyu Zhang Yunyun Pan Chenglin Xie Tonghui Xie Yongkui Zhang Changhong Yao |
| author_facet | Xiuyuan Ran Yuhan Shen Dongjian Jiang Chenqi Wang Xinghui Li Haoyu Zhang Yunyun Pan Chenglin Xie Tonghui Xie Yongkui Zhang Changhong Yao |
| author_sort | Xiuyuan Ran |
| collection | DOAJ |
| description | Chrysolaminarin, a kind of water-soluble bioactive β-glucan produced by certain microalgae, is a potential candidate for food/pharmaceutical applications. This study identified a marine microalga <i>Isochrysis zhangjiangensis</i>, in which chrysolaminarin production was investigated via nutrient (nitrogen, phosphorus, or sulfur) deprivations (-N, -P, or -S conditions) along with an increase in light intensity. A characterization of the antioxidant activities of the chrysolaminarin produced under each condition was also conducted. The results showed that nutrient deprivation caused a significant increase in chrysolaminarin accumulation, though this was accompanied by diminished biomass production and photosynthetic activity. -S was the best strategy to induce chrysolaminarin accumulation. An increase in light intensity from 80 (LL) to 150 (HL) µE·m<sup>−2</sup>·s<sup>−1</sup> further enhanced chrysolaminarin production. Compared with -N, -S caused more suitable stress and reduced carbon allocation toward neutral lipid production, which enabled a higher chrysolaminarin accumulation capacity. The highest chrysolaminarin content and concentration reached 41.7% of dry weight (%DW) and 632.2 mg/L, respectively, under HL-S, with a corresponding productivity of 155.1 mg/L/day achieved, which exceeds most of the photoautotrophic microalgae previously reported. The chrysolaminarin produced under HL-N (Iz-N) had a relatively competitive hydroxyl radical scavenging activity at low concentrations, while the chrysolaminarin produced under HL-S (Iz-S) exhibited an overall better activity, comparable to the commercial yeast β-glucan, demonstrating <i>I. zhangjiangensis</i> as a promising bioactive chrysolaminarin producer from CO<sub>2</sub>. |
| first_indexed | 2024-03-09T23:13:32Z |
| format | Article |
| id | doaj.art-4884b61ef32f476dbda11b18dde8ffcb |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T23:13:32Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-4884b61ef32f476dbda11b18dde8ffcb2023-11-23T17:40:44ZengMDPI AGMarine Drugs1660-33972022-05-0120635110.3390/md20060351Nutrient Deprivation Coupled with High Light Exposure for Bioactive Chrysolaminarin Production in the Marine Microalga <i>Isochrysis zhangjiangensis</i>Xiuyuan Ran0Yuhan Shen1Dongjian Jiang2Chenqi Wang3Xinghui Li4Haoyu Zhang5Yunyun Pan6Chenglin Xie7Tonghui Xie8Yongkui Zhang9Changhong Yao10Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, ChinaDepartment of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, ChinaDepartment of Chemical Technology, School of Chemical Engineering, Sichuan University, Chengdu 610065, ChinaDepartment of Chemical Technology, School of Chemical Engineering, Sichuan University, Chengdu 610065, ChinaWest China School of Pharmacy, Sichuan University, Chengdu 610041, ChinaDepartment of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, ChinaDepartment of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, ChinaDepartment of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, ChinaDepartment of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, ChinaDepartment of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, ChinaDepartment of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, ChinaChrysolaminarin, a kind of water-soluble bioactive β-glucan produced by certain microalgae, is a potential candidate for food/pharmaceutical applications. This study identified a marine microalga <i>Isochrysis zhangjiangensis</i>, in which chrysolaminarin production was investigated via nutrient (nitrogen, phosphorus, or sulfur) deprivations (-N, -P, or -S conditions) along with an increase in light intensity. A characterization of the antioxidant activities of the chrysolaminarin produced under each condition was also conducted. The results showed that nutrient deprivation caused a significant increase in chrysolaminarin accumulation, though this was accompanied by diminished biomass production and photosynthetic activity. -S was the best strategy to induce chrysolaminarin accumulation. An increase in light intensity from 80 (LL) to 150 (HL) µE·m<sup>−2</sup>·s<sup>−1</sup> further enhanced chrysolaminarin production. Compared with -N, -S caused more suitable stress and reduced carbon allocation toward neutral lipid production, which enabled a higher chrysolaminarin accumulation capacity. The highest chrysolaminarin content and concentration reached 41.7% of dry weight (%DW) and 632.2 mg/L, respectively, under HL-S, with a corresponding productivity of 155.1 mg/L/day achieved, which exceeds most of the photoautotrophic microalgae previously reported. The chrysolaminarin produced under HL-N (Iz-N) had a relatively competitive hydroxyl radical scavenging activity at low concentrations, while the chrysolaminarin produced under HL-S (Iz-S) exhibited an overall better activity, comparable to the commercial yeast β-glucan, demonstrating <i>I. zhangjiangensis</i> as a promising bioactive chrysolaminarin producer from CO<sub>2</sub>.https://www.mdpi.com/1660-3397/20/6/351chrysolaminarin<i>Isochrysis zhangjiangensis</i>nutrient deprivationlight intensityantioxidant |
| spellingShingle | Xiuyuan Ran Yuhan Shen Dongjian Jiang Chenqi Wang Xinghui Li Haoyu Zhang Yunyun Pan Chenglin Xie Tonghui Xie Yongkui Zhang Changhong Yao Nutrient Deprivation Coupled with High Light Exposure for Bioactive Chrysolaminarin Production in the Marine Microalga <i>Isochrysis zhangjiangensis</i> Marine Drugs chrysolaminarin <i>Isochrysis zhangjiangensis</i> nutrient deprivation light intensity antioxidant |
| title | Nutrient Deprivation Coupled with High Light Exposure for Bioactive Chrysolaminarin Production in the Marine Microalga <i>Isochrysis zhangjiangensis</i> |
| title_full | Nutrient Deprivation Coupled with High Light Exposure for Bioactive Chrysolaminarin Production in the Marine Microalga <i>Isochrysis zhangjiangensis</i> |
| title_fullStr | Nutrient Deprivation Coupled with High Light Exposure for Bioactive Chrysolaminarin Production in the Marine Microalga <i>Isochrysis zhangjiangensis</i> |
| title_full_unstemmed | Nutrient Deprivation Coupled with High Light Exposure for Bioactive Chrysolaminarin Production in the Marine Microalga <i>Isochrysis zhangjiangensis</i> |
| title_short | Nutrient Deprivation Coupled with High Light Exposure for Bioactive Chrysolaminarin Production in the Marine Microalga <i>Isochrysis zhangjiangensis</i> |
| title_sort | nutrient deprivation coupled with high light exposure for bioactive chrysolaminarin production in the marine microalga i isochrysis zhangjiangensis i |
| topic | chrysolaminarin <i>Isochrysis zhangjiangensis</i> nutrient deprivation light intensity antioxidant |
| url | https://www.mdpi.com/1660-3397/20/6/351 |
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