Semi-automated water sampling module for repeated sampling and concentration of Bacillus cereus group spores
Abstract Monitoring the presence of pathogenic Bacillus spores is important for industrial applications, as well as necessary for ensuring human health. Bacillus thuringiensis is used as a biopesticide against several insect pests. Bacillus cereus spores are a significant cause of food poisoning, an...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-27900-0 |
| _version_ | 1797945941087485952 |
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| author | Walid M. Hassen Jonathan Vermette Houman Moteshareie Azam F. Tayabali Jan J. Dubowski |
| author_facet | Walid M. Hassen Jonathan Vermette Houman Moteshareie Azam F. Tayabali Jan J. Dubowski |
| author_sort | Walid M. Hassen |
| collection | DOAJ |
| description | Abstract Monitoring the presence of pathogenic Bacillus spores is important for industrial applications, as well as necessary for ensuring human health. Bacillus thuringiensis is used as a biopesticide against several insect pests. Bacillus cereus spores are a significant cause of food poisoning, and Bacillus anthracis is a recognized biosecurity threat. Laboratory-based methods, such as polymerase chain reaction, enzyme-linked immunosorbent assay, or matrix-assisted laser desorption ionization spectroscopy provide sensitive detection of bacteria and spores, but the application of those methods for quasi-continuous environmental monitoring presents a significant challenge requiring frequent human intervention. To address this challenge, we developed a workstation for quasi-autonomous monitoring of water reservoirs for the presence of bacteria and spores, and designed and validated the functionality of a microprocessor-controlled module capable of repetitive collection and pre-concentration of spores in liquid samples tested with fiberglass (FG), polyether sulfone and polyvinylidene fluoride filters. The best results were obtained with FG filters delivering a 20× concentration of B. thuringiensis and B. cereus spores from saline suspensions. The successful 20× pre-concentration of Bacillus spores demonstrated with FG filters could be repeated up to 3 times when bleach decontamination is applied between filtrations. Taken together, our results demonstrate an attractive instrument suitable for semi-automated, quasi-continuous sampling and pre-processing of water samples for biosensing of bacterial spores originating from a complex environment. |
| first_indexed | 2024-04-10T21:03:00Z |
| format | Article |
| id | doaj.art-48859c6cbef34b6d8e87aaf419358a3a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T21:03:00Z |
| publishDate | 2023-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-48859c6cbef34b6d8e87aaf419358a3a2023-01-22T12:09:51ZengNature PortfolioScientific Reports2045-23222023-01-011311910.1038/s41598-023-27900-0Semi-automated water sampling module for repeated sampling and concentration of Bacillus cereus group sporesWalid M. Hassen0Jonathan Vermette1Houman Moteshareie2Azam F. Tayabali3Jan J. Dubowski4Laboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Department of Electrical and Computer Engineering, Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Université de SherbrookeLaboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Department of Electrical and Computer Engineering, Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Université de SherbrookeLaboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Department of Electrical and Computer Engineering, Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Université de SherbrookeLaboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Department of Electrical and Computer Engineering, Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Université de SherbrookeLaboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Department of Electrical and Computer Engineering, Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Université de SherbrookeAbstract Monitoring the presence of pathogenic Bacillus spores is important for industrial applications, as well as necessary for ensuring human health. Bacillus thuringiensis is used as a biopesticide against several insect pests. Bacillus cereus spores are a significant cause of food poisoning, and Bacillus anthracis is a recognized biosecurity threat. Laboratory-based methods, such as polymerase chain reaction, enzyme-linked immunosorbent assay, or matrix-assisted laser desorption ionization spectroscopy provide sensitive detection of bacteria and spores, but the application of those methods for quasi-continuous environmental monitoring presents a significant challenge requiring frequent human intervention. To address this challenge, we developed a workstation for quasi-autonomous monitoring of water reservoirs for the presence of bacteria and spores, and designed and validated the functionality of a microprocessor-controlled module capable of repetitive collection and pre-concentration of spores in liquid samples tested with fiberglass (FG), polyether sulfone and polyvinylidene fluoride filters. The best results were obtained with FG filters delivering a 20× concentration of B. thuringiensis and B. cereus spores from saline suspensions. The successful 20× pre-concentration of Bacillus spores demonstrated with FG filters could be repeated up to 3 times when bleach decontamination is applied between filtrations. Taken together, our results demonstrate an attractive instrument suitable for semi-automated, quasi-continuous sampling and pre-processing of water samples for biosensing of bacterial spores originating from a complex environment.https://doi.org/10.1038/s41598-023-27900-0 |
| spellingShingle | Walid M. Hassen Jonathan Vermette Houman Moteshareie Azam F. Tayabali Jan J. Dubowski Semi-automated water sampling module for repeated sampling and concentration of Bacillus cereus group spores Scientific Reports |
| title | Semi-automated water sampling module for repeated sampling and concentration of Bacillus cereus group spores |
| title_full | Semi-automated water sampling module for repeated sampling and concentration of Bacillus cereus group spores |
| title_fullStr | Semi-automated water sampling module for repeated sampling and concentration of Bacillus cereus group spores |
| title_full_unstemmed | Semi-automated water sampling module for repeated sampling and concentration of Bacillus cereus group spores |
| title_short | Semi-automated water sampling module for repeated sampling and concentration of Bacillus cereus group spores |
| title_sort | semi automated water sampling module for repeated sampling and concentration of bacillus cereus group spores |
| url | https://doi.org/10.1038/s41598-023-27900-0 |
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