Humoral responses to SARS-CoV-2 by healthy and sick dogs during the COVID-19 pandemic in Spain
Abstract COVID-19 is a zoonotic disease caused by SARS-CoV-2. Infections of animals with SARS-CoV-2 have recently been reported, and an increase of severe lung pathologies in domestic dogs has also been detected by veterinarians in Spain. Therefore, further descriptions of the pathological processes...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-02-01
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| Series: | Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13567-021-00897-y |
| _version_ | 1818953330666242048 |
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| author | Ana Judith Perisé-Barrios Beatriz Davinia Tomeo-Martín Pablo Gómez-Ochoa Pablo Delgado-Bonet Pedro Plaza Paula Palau-Concejo Jorge González Gustavo Ortiz-Díez Antonio Meléndez-Lazo Michaela Gentil Javier García-Castro Alicia Barbero-Fernández |
| author_facet | Ana Judith Perisé-Barrios Beatriz Davinia Tomeo-Martín Pablo Gómez-Ochoa Pablo Delgado-Bonet Pedro Plaza Paula Palau-Concejo Jorge González Gustavo Ortiz-Díez Antonio Meléndez-Lazo Michaela Gentil Javier García-Castro Alicia Barbero-Fernández |
| author_sort | Ana Judith Perisé-Barrios |
| collection | DOAJ |
| description | Abstract COVID-19 is a zoonotic disease caused by SARS-CoV-2. Infections of animals with SARS-CoV-2 have recently been reported, and an increase of severe lung pathologies in domestic dogs has also been detected by veterinarians in Spain. Therefore, further descriptions of the pathological processes in those animals that show symptoms similar to those described in humans affected by COVID-19 would be highly valuable. The potential for companion animals to contribute to the continued transmission and community spread of this known human-to-human disease is an urgent issue to be considered. Forty animals with pulmonary pathologies were studied by chest X-ray, ultrasound analysis, and computed tomography. Nasopharyngeal and rectal swabs were analyzed to detect canine pathogens, including SARS-CoV-2. An additional twenty healthy dogs living in SARS-CoV-2-positive households were included. Immunoglobulin detection by several immunoassays was performed. Our findings show that sick dogs presented severe alveolar or interstitial patterns with pulmonary opacity, parenchymal abnormalities, and bilateral lesions. The forty sick dogs were negative for SARS-CoV-2 but Mycoplasma spp. was detected in 26 of 33 dogs. Five healthy and one pathological dog presented IgG against SARS-CoV-2. Here we report that despite detecting dogs with α-SARS-CoV-2 IgG, we never obtained a positive RT-qPCR for SARS-SoV-2, not even in dogs with severe pulmonary disease; suggesting that even in the case of canine infection, transmission would be unlikely. Moreover, dogs living in COVID-19-positive households could have been more highly exposed to infection with SARS-CoV-2. |
| first_indexed | 2024-12-20T10:04:34Z |
| format | Article |
| id | doaj.art-4885dadfb30447cda44ccc975ce7f5fb |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-12-20T10:04:34Z |
| publishDate | 2021-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-4885dadfb30447cda44ccc975ce7f5fb2022-12-21T19:44:14ZengBMCVeterinary Research1297-97162021-02-0152111110.1186/s13567-021-00897-yHumoral responses to SARS-CoV-2 by healthy and sick dogs during the COVID-19 pandemic in SpainAna Judith Perisé-Barrios0Beatriz Davinia Tomeo-Martín1Pablo Gómez-Ochoa2Pablo Delgado-Bonet3Pedro Plaza4Paula Palau-Concejo5Jorge González6Gustavo Ortiz-Díez7Antonio Meléndez-Lazo8Michaela Gentil9Javier García-Castro10Alicia Barbero-Fernández11Biomedical Research Unit, Universidad Alfonso X El SabioBiomedical Research Unit, Universidad Alfonso X El SabioVetcornerBiomedical Research Unit, Universidad Alfonso X El SabioERVET-DIEZ BRUBiomedical Research Unit, Universidad Alfonso X El SabioMicros Veterinaria S.L., Calle Profesor Pedro CármenesFaculty of Veterinary, Universidad Alfonso X El SabioLaboklin GmbH & Co. KGLaboklin GmbH & Co. KGCellular Biotechnology Unit, Instituto de Salud Carlos IIIFaculty of Veterinary, Universidad Alfonso X El SabioAbstract COVID-19 is a zoonotic disease caused by SARS-CoV-2. Infections of animals with SARS-CoV-2 have recently been reported, and an increase of severe lung pathologies in domestic dogs has also been detected by veterinarians in Spain. Therefore, further descriptions of the pathological processes in those animals that show symptoms similar to those described in humans affected by COVID-19 would be highly valuable. The potential for companion animals to contribute to the continued transmission and community spread of this known human-to-human disease is an urgent issue to be considered. Forty animals with pulmonary pathologies were studied by chest X-ray, ultrasound analysis, and computed tomography. Nasopharyngeal and rectal swabs were analyzed to detect canine pathogens, including SARS-CoV-2. An additional twenty healthy dogs living in SARS-CoV-2-positive households were included. Immunoglobulin detection by several immunoassays was performed. Our findings show that sick dogs presented severe alveolar or interstitial patterns with pulmonary opacity, parenchymal abnormalities, and bilateral lesions. The forty sick dogs were negative for SARS-CoV-2 but Mycoplasma spp. was detected in 26 of 33 dogs. Five healthy and one pathological dog presented IgG against SARS-CoV-2. Here we report that despite detecting dogs with α-SARS-CoV-2 IgG, we never obtained a positive RT-qPCR for SARS-SoV-2, not even in dogs with severe pulmonary disease; suggesting that even in the case of canine infection, transmission would be unlikely. Moreover, dogs living in COVID-19-positive households could have been more highly exposed to infection with SARS-CoV-2.https://doi.org/10.1186/s13567-021-00897-ySARS-CoV-2DogsMycoplasmaAntibodyPneumonia |
| spellingShingle | Ana Judith Perisé-Barrios Beatriz Davinia Tomeo-Martín Pablo Gómez-Ochoa Pablo Delgado-Bonet Pedro Plaza Paula Palau-Concejo Jorge González Gustavo Ortiz-Díez Antonio Meléndez-Lazo Michaela Gentil Javier García-Castro Alicia Barbero-Fernández Humoral responses to SARS-CoV-2 by healthy and sick dogs during the COVID-19 pandemic in Spain Veterinary Research SARS-CoV-2 Dogs Mycoplasma Antibody Pneumonia |
| title | Humoral responses to SARS-CoV-2 by healthy and sick dogs during the COVID-19 pandemic in Spain |
| title_full | Humoral responses to SARS-CoV-2 by healthy and sick dogs during the COVID-19 pandemic in Spain |
| title_fullStr | Humoral responses to SARS-CoV-2 by healthy and sick dogs during the COVID-19 pandemic in Spain |
| title_full_unstemmed | Humoral responses to SARS-CoV-2 by healthy and sick dogs during the COVID-19 pandemic in Spain |
| title_short | Humoral responses to SARS-CoV-2 by healthy and sick dogs during the COVID-19 pandemic in Spain |
| title_sort | humoral responses to sars cov 2 by healthy and sick dogs during the covid 19 pandemic in spain |
| topic | SARS-CoV-2 Dogs Mycoplasma Antibody Pneumonia |
| url | https://doi.org/10.1186/s13567-021-00897-y |
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