G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla
With an internal transcribed spacer of 18 S, 5.8 S and 26 S nuclear ribosomal DNA (nrDNA ITS) as DNA marker, we report a colorimetric approach for authentication of Pseudostellaria heterophylla (PH) and its counterfeit species based on the differentiation of the nrDNA ITS sequence. The assay possess...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-01-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/13/1/1064 |
| _version_ | 1798034640507764736 |
|---|---|
| author | Juan Hu Wensheng Pang Zhenzhu Zheng Jing Han |
| author_facet | Juan Hu Wensheng Pang Zhenzhu Zheng Jing Han |
| author_sort | Juan Hu |
| collection | DOAJ |
| description | With an internal transcribed spacer of 18 S, 5.8 S and 26 S nuclear ribosomal DNA (nrDNA ITS) as DNA marker, we report a colorimetric approach for authentication of Pseudostellaria heterophylla (PH) and its counterfeit species based on the differentiation of the nrDNA ITS sequence. The assay possesses an unlabelled G-quadruplex DNAzyme molecular beacon (MB) probe, employing complementary sequence as biorecognition element and 1:1:1:1 split G-quadruplex halves as reporter. In the absence of target DNA (T-DNA), the probe can shape intermolecular G-quadruplex structures capable of binding hemin to form G-quadruplex-hemin DNAzyme and catalyze the oxidation of ABTS2− to blue-green ABTS•− by H2O2. In the presence of T-DNA, T-DNA can hybridize with the complementary sequence to form a duplex structure, hindering the formation of the G-quadruplex structure and resulting in the loss of the catalytic activity. Consequently, a UV-Vis absorption signal decrease is observed in the ABTS2−-H2O2 system. The “turn-off” assay allows the detection of T-DNA from 1.0 × 10−9 to 3.0 × 10−7 mol·L−1 (R2 = 0.9906), with a low detection limit of 3.1 × 10−10 mol·L−1. The present study provides a sensitive and selective method and may serve as a foundation of utilizing the DNAzyme MB sensor for identifying traditional Chinese medicines. |
| first_indexed | 2024-04-11T20:47:01Z |
| format | Article |
| id | doaj.art-4886badfafe248f2895e345ab0571270 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T20:47:01Z |
| publishDate | 2013-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-4886badfafe248f2895e345ab05712702022-12-22T04:04:00ZengMDPI AGSensors1424-82202013-01-011311064107510.3390/s130101064G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophyllaJuan HuWensheng PangZhenzhu ZhengJing HanWith an internal transcribed spacer of 18 S, 5.8 S and 26 S nuclear ribosomal DNA (nrDNA ITS) as DNA marker, we report a colorimetric approach for authentication of Pseudostellaria heterophylla (PH) and its counterfeit species based on the differentiation of the nrDNA ITS sequence. The assay possesses an unlabelled G-quadruplex DNAzyme molecular beacon (MB) probe, employing complementary sequence as biorecognition element and 1:1:1:1 split G-quadruplex halves as reporter. In the absence of target DNA (T-DNA), the probe can shape intermolecular G-quadruplex structures capable of binding hemin to form G-quadruplex-hemin DNAzyme and catalyze the oxidation of ABTS2− to blue-green ABTS•− by H2O2. In the presence of T-DNA, T-DNA can hybridize with the complementary sequence to form a duplex structure, hindering the formation of the G-quadruplex structure and resulting in the loss of the catalytic activity. Consequently, a UV-Vis absorption signal decrease is observed in the ABTS2−-H2O2 system. The “turn-off” assay allows the detection of T-DNA from 1.0 × 10−9 to 3.0 × 10−7 mol·L−1 (R2 = 0.9906), with a low detection limit of 3.1 × 10−10 mol·L−1. The present study provides a sensitive and selective method and may serve as a foundation of utilizing the DNAzyme MB sensor for identifying traditional Chinese medicines.http://www.mdpi.com/1424-8220/13/1/1064G-quadruplex DNAzymemolecular beaconcolorimetricPseudostellaria heterophylla |
| spellingShingle | Juan Hu Wensheng Pang Zhenzhu Zheng Jing Han G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla Sensors G-quadruplex DNAzyme molecular beacon colorimetric Pseudostellaria heterophylla |
| title | G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla |
| title_full | G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla |
| title_fullStr | G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla |
| title_full_unstemmed | G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla |
| title_short | G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla |
| title_sort | g quadruplex dnazyme molecular beacon for amplified colorimetric biosensing of pseudostellaria heterophylla |
| topic | G-quadruplex DNAzyme molecular beacon colorimetric Pseudostellaria heterophylla |
| url | http://www.mdpi.com/1424-8220/13/1/1064 |
| work_keys_str_mv | AT juanhu gquadruplexdnazymemolecularbeaconforamplifiedcolorimetricbiosensingofpseudostellariaheterophylla AT wenshengpang gquadruplexdnazymemolecularbeaconforamplifiedcolorimetricbiosensingofpseudostellariaheterophylla AT zhenzhuzheng gquadruplexdnazymemolecularbeaconforamplifiedcolorimetricbiosensingofpseudostellariaheterophylla AT jinghan gquadruplexdnazymemolecularbeaconforamplifiedcolorimetricbiosensingofpseudostellariaheterophylla |